In vitro selection of Plasmodium falciparum drug-resistant parasite lines

The in vitro selection of antimicrobial resistance in important pathogens can provide critical information on the genetic basis of drug resistance, and such information can be used to predict, anticipate and even contain the spread of resistance in clinical practice. For instance, the discovery of t...

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Detalles Bibliográficos
Autores principales: Nzila, Alexis, Mwai, Leah
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Reviews
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2818104/
https://www.ncbi.nlm.nih.gov/pubmed/20022938
http://dx.doi.org/10.1093/jac/dkp449
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author Nzila, Alexis
Mwai, Leah
author_facet Nzila, Alexis
Mwai, Leah
author_sort Nzila, Alexis
collection PubMed
description The in vitro selection of antimicrobial resistance in important pathogens can provide critical information on the genetic basis of drug resistance, and such information can be used to predict, anticipate and even contain the spread of resistance in clinical practice. For instance, the discovery of the role of pfmdr1 in mefloquine resistance in malaria parasites resulted from in vitro studies. However, the in vitro selection of resistance is difficult, challenging and time consuming. In this review, we discuss the key parameters that impact on the efficiency of the in vitro selection of resistance, and propose strategies to improve and streamline this process.
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spelling pubmed-28181042010-02-10 In vitro selection of Plasmodium falciparum drug-resistant parasite lines Nzila, Alexis Mwai, Leah J Antimicrob Chemother Reviews The in vitro selection of antimicrobial resistance in important pathogens can provide critical information on the genetic basis of drug resistance, and such information can be used to predict, anticipate and even contain the spread of resistance in clinical practice. For instance, the discovery of the role of pfmdr1 in mefloquine resistance in malaria parasites resulted from in vitro studies. However, the in vitro selection of resistance is difficult, challenging and time consuming. In this review, we discuss the key parameters that impact on the efficiency of the in vitro selection of resistance, and propose strategies to improve and streamline this process. Oxford University Press 2010-03 2009-12-18 /pmc/articles/PMC2818104/ /pubmed/20022938 http://dx.doi.org/10.1093/jac/dkp449 Text en © The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Reviews
Nzila, Alexis
Mwai, Leah
In vitro selection of Plasmodium falciparum drug-resistant parasite lines
title In vitro selection of Plasmodium falciparum drug-resistant parasite lines
title_full In vitro selection of Plasmodium falciparum drug-resistant parasite lines
title_fullStr In vitro selection of Plasmodium falciparum drug-resistant parasite lines
title_full_unstemmed In vitro selection of Plasmodium falciparum drug-resistant parasite lines
title_short In vitro selection of Plasmodium falciparum drug-resistant parasite lines
title_sort in vitro selection of plasmodium falciparum drug-resistant parasite lines
topic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2818104/
https://www.ncbi.nlm.nih.gov/pubmed/20022938
http://dx.doi.org/10.1093/jac/dkp449
work_keys_str_mv AT nzilaalexis invitroselectionofplasmodiumfalciparumdrugresistantparasitelines
AT mwaileah invitroselectionofplasmodiumfalciparumdrugresistantparasitelines

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