Cargando…

Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections

BACKGROUND: The insecticides dichlorvos, paradichlorobenzene and naphthalene have been commonly used to eradicate pest insects from natural history collections. However, it is not known how these chemicals affect the DNA of the specimens in the collections. We thus tested the effect of dichlorvos, p...

Descripción completa

Detalles Bibliográficos
Autores principales: Espeland, Marianne, Irestedt, Martin, Johanson, Kjell Arne, Åkerlund, Monika, Bergh, Jan-Erik, Källersjö, Mari
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2819063/
https://www.ncbi.nlm.nih.gov/pubmed/20148102
http://dx.doi.org/10.1186/1742-9994-7-2
_version_ 1782177288968732672
author Espeland, Marianne
Irestedt, Martin
Johanson, Kjell Arne
Åkerlund, Monika
Bergh, Jan-Erik
Källersjö, Mari
author_facet Espeland, Marianne
Irestedt, Martin
Johanson, Kjell Arne
Åkerlund, Monika
Bergh, Jan-Erik
Källersjö, Mari
author_sort Espeland, Marianne
collection PubMed
description BACKGROUND: The insecticides dichlorvos, paradichlorobenzene and naphthalene have been commonly used to eradicate pest insects from natural history collections. However, it is not known how these chemicals affect the DNA of the specimens in the collections. We thus tested the effect of dichlorvos, paradichlorobenzene and naphthalene on DNA of insects (Musca domestica) by extracting and amplifying DNA from specimens exposed to insecticides in two different concentrations over increasing time intervals. RESULTS: The results clearly show that dichlorvos impedes both extraction and amplification of mitochondrial and nuclear DNA after relatively short time, whereas paradichlorobenzene and naphthalene do not. CONCLUSION: Collections treated with paradichlorobenzene and naphthalene, are better preserved concerning DNA, than those treated with dichlorvos. Non toxic pest control methods should, however, be preferred due to physical damage of specimens and putative health risks by chemicals.
format Text
id pubmed-2819063
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-28190632010-02-10 Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections Espeland, Marianne Irestedt, Martin Johanson, Kjell Arne Åkerlund, Monika Bergh, Jan-Erik Källersjö, Mari Front Zool Methodology BACKGROUND: The insecticides dichlorvos, paradichlorobenzene and naphthalene have been commonly used to eradicate pest insects from natural history collections. However, it is not known how these chemicals affect the DNA of the specimens in the collections. We thus tested the effect of dichlorvos, paradichlorobenzene and naphthalene on DNA of insects (Musca domestica) by extracting and amplifying DNA from specimens exposed to insecticides in two different concentrations over increasing time intervals. RESULTS: The results clearly show that dichlorvos impedes both extraction and amplification of mitochondrial and nuclear DNA after relatively short time, whereas paradichlorobenzene and naphthalene do not. CONCLUSION: Collections treated with paradichlorobenzene and naphthalene, are better preserved concerning DNA, than those treated with dichlorvos. Non toxic pest control methods should, however, be preferred due to physical damage of specimens and putative health risks by chemicals. BioMed Central 2010-01-18 /pmc/articles/PMC2819063/ /pubmed/20148102 http://dx.doi.org/10.1186/1742-9994-7-2 Text en Copyright ©2010 Espeland et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Espeland, Marianne
Irestedt, Martin
Johanson, Kjell Arne
Åkerlund, Monika
Bergh, Jan-Erik
Källersjö, Mari
Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections
title Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections
title_full Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections
title_fullStr Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections
title_full_unstemmed Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections
title_short Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections
title_sort dichlorvos exposure impedes extraction and amplification of dna from insects in museum collections
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2819063/
https://www.ncbi.nlm.nih.gov/pubmed/20148102
http://dx.doi.org/10.1186/1742-9994-7-2
work_keys_str_mv AT espelandmarianne dichlorvosexposureimpedesextractionandamplificationofdnafrominsectsinmuseumcollections
AT irestedtmartin dichlorvosexposureimpedesextractionandamplificationofdnafrominsectsinmuseumcollections
AT johansonkjellarne dichlorvosexposureimpedesextractionandamplificationofdnafrominsectsinmuseumcollections
AT akerlundmonika dichlorvosexposureimpedesextractionandamplificationofdnafrominsectsinmuseumcollections
AT berghjanerik dichlorvosexposureimpedesextractionandamplificationofdnafrominsectsinmuseumcollections
AT kallersjomari dichlorvosexposureimpedesextractionandamplificationofdnafrominsectsinmuseumcollections