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Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections
BACKGROUND: The insecticides dichlorvos, paradichlorobenzene and naphthalene have been commonly used to eradicate pest insects from natural history collections. However, it is not known how these chemicals affect the DNA of the specimens in the collections. We thus tested the effect of dichlorvos, p...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2819063/ https://www.ncbi.nlm.nih.gov/pubmed/20148102 http://dx.doi.org/10.1186/1742-9994-7-2 |
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author | Espeland, Marianne Irestedt, Martin Johanson, Kjell Arne Åkerlund, Monika Bergh, Jan-Erik Källersjö, Mari |
author_facet | Espeland, Marianne Irestedt, Martin Johanson, Kjell Arne Åkerlund, Monika Bergh, Jan-Erik Källersjö, Mari |
author_sort | Espeland, Marianne |
collection | PubMed |
description | BACKGROUND: The insecticides dichlorvos, paradichlorobenzene and naphthalene have been commonly used to eradicate pest insects from natural history collections. However, it is not known how these chemicals affect the DNA of the specimens in the collections. We thus tested the effect of dichlorvos, paradichlorobenzene and naphthalene on DNA of insects (Musca domestica) by extracting and amplifying DNA from specimens exposed to insecticides in two different concentrations over increasing time intervals. RESULTS: The results clearly show that dichlorvos impedes both extraction and amplification of mitochondrial and nuclear DNA after relatively short time, whereas paradichlorobenzene and naphthalene do not. CONCLUSION: Collections treated with paradichlorobenzene and naphthalene, are better preserved concerning DNA, than those treated with dichlorvos. Non toxic pest control methods should, however, be preferred due to physical damage of specimens and putative health risks by chemicals. |
format | Text |
id | pubmed-2819063 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28190632010-02-10 Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections Espeland, Marianne Irestedt, Martin Johanson, Kjell Arne Åkerlund, Monika Bergh, Jan-Erik Källersjö, Mari Front Zool Methodology BACKGROUND: The insecticides dichlorvos, paradichlorobenzene and naphthalene have been commonly used to eradicate pest insects from natural history collections. However, it is not known how these chemicals affect the DNA of the specimens in the collections. We thus tested the effect of dichlorvos, paradichlorobenzene and naphthalene on DNA of insects (Musca domestica) by extracting and amplifying DNA from specimens exposed to insecticides in two different concentrations over increasing time intervals. RESULTS: The results clearly show that dichlorvos impedes both extraction and amplification of mitochondrial and nuclear DNA after relatively short time, whereas paradichlorobenzene and naphthalene do not. CONCLUSION: Collections treated with paradichlorobenzene and naphthalene, are better preserved concerning DNA, than those treated with dichlorvos. Non toxic pest control methods should, however, be preferred due to physical damage of specimens and putative health risks by chemicals. BioMed Central 2010-01-18 /pmc/articles/PMC2819063/ /pubmed/20148102 http://dx.doi.org/10.1186/1742-9994-7-2 Text en Copyright ©2010 Espeland et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methodology Espeland, Marianne Irestedt, Martin Johanson, Kjell Arne Åkerlund, Monika Bergh, Jan-Erik Källersjö, Mari Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections |
title | Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections |
title_full | Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections |
title_fullStr | Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections |
title_full_unstemmed | Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections |
title_short | Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections |
title_sort | dichlorvos exposure impedes extraction and amplification of dna from insects in museum collections |
topic | Methodology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2819063/ https://www.ncbi.nlm.nih.gov/pubmed/20148102 http://dx.doi.org/10.1186/1742-9994-7-2 |
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