Cargando…

Two Isoforms of Npap60 (Nup50) Differentially Regulate Nuclear Protein Import

Npap60 (Nup50) is a nucleoporin that binds directly to importin α. In humans, there are two Npap60 isoforms: the long (Npap60L) and short (Npap60S) forms. In this study, we provide both in vitro and in vivo evidence that Npap60L and Npap60S function differently in nuclear protein import. In vitro bi...

Descripción completa

Detalles Bibliográficos
Autores principales: Ogawa, Yutaka, Miyamoto, Yoichi, Asally, Munehiro, Oka, Masahiro, Yasuda, Yoshinari, Yoneda, Yoshihiro
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2820426/
https://www.ncbi.nlm.nih.gov/pubmed/20016008
http://dx.doi.org/10.1091/mbc.E09-05-0374
_version_ 1782177365855567872
author Ogawa, Yutaka
Miyamoto, Yoichi
Asally, Munehiro
Oka, Masahiro
Yasuda, Yoshinari
Yoneda, Yoshihiro
author_facet Ogawa, Yutaka
Miyamoto, Yoichi
Asally, Munehiro
Oka, Masahiro
Yasuda, Yoshinari
Yoneda, Yoshihiro
author_sort Ogawa, Yutaka
collection PubMed
description Npap60 (Nup50) is a nucleoporin that binds directly to importin α. In humans, there are two Npap60 isoforms: the long (Npap60L) and short (Npap60S) forms. In this study, we provide both in vitro and in vivo evidence that Npap60L and Npap60S function differently in nuclear protein import. In vitro binding assays revealed that Npap60S stabilizes the binding of importin α to classical NLS-cargo, whereas Npap60L promotes the release of NLS-cargo from importin α. In vivo time-lapse experiments showed that when the Npap60 protein level is controlled, allowing CAS to efficiently promote the dissociation of the Npap60/importin α complex, Npap60S and Npap60L suppress and accelerate the nuclear import of NLS-cargo, respectively. These results demonstrate that Npap60L and Npap60S have opposing functions and suggest that Npap60L and Npap60S levels must be carefully controlled for efficient nuclear import of classical NLS-cargo in humans. This study provides novel evidence that nucleoporin expression levels regulate nuclear import efficiency.
format Text
id pubmed-2820426
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-28204262010-04-30 Two Isoforms of Npap60 (Nup50) Differentially Regulate Nuclear Protein Import Ogawa, Yutaka Miyamoto, Yoichi Asally, Munehiro Oka, Masahiro Yasuda, Yoshinari Yoneda, Yoshihiro Mol Biol Cell Articles Npap60 (Nup50) is a nucleoporin that binds directly to importin α. In humans, there are two Npap60 isoforms: the long (Npap60L) and short (Npap60S) forms. In this study, we provide both in vitro and in vivo evidence that Npap60L and Npap60S function differently in nuclear protein import. In vitro binding assays revealed that Npap60S stabilizes the binding of importin α to classical NLS-cargo, whereas Npap60L promotes the release of NLS-cargo from importin α. In vivo time-lapse experiments showed that when the Npap60 protein level is controlled, allowing CAS to efficiently promote the dissociation of the Npap60/importin α complex, Npap60S and Npap60L suppress and accelerate the nuclear import of NLS-cargo, respectively. These results demonstrate that Npap60L and Npap60S have opposing functions and suggest that Npap60L and Npap60S levels must be carefully controlled for efficient nuclear import of classical NLS-cargo in humans. This study provides novel evidence that nucleoporin expression levels regulate nuclear import efficiency. The American Society for Cell Biology 2010-02-15 /pmc/articles/PMC2820426/ /pubmed/20016008 http://dx.doi.org/10.1091/mbc.E09-05-0374 Text en © 2010 by The American Society for Cell Biology
spellingShingle Articles
Ogawa, Yutaka
Miyamoto, Yoichi
Asally, Munehiro
Oka, Masahiro
Yasuda, Yoshinari
Yoneda, Yoshihiro
Two Isoforms of Npap60 (Nup50) Differentially Regulate Nuclear Protein Import
title Two Isoforms of Npap60 (Nup50) Differentially Regulate Nuclear Protein Import
title_full Two Isoforms of Npap60 (Nup50) Differentially Regulate Nuclear Protein Import
title_fullStr Two Isoforms of Npap60 (Nup50) Differentially Regulate Nuclear Protein Import
title_full_unstemmed Two Isoforms of Npap60 (Nup50) Differentially Regulate Nuclear Protein Import
title_short Two Isoforms of Npap60 (Nup50) Differentially Regulate Nuclear Protein Import
title_sort two isoforms of npap60 (nup50) differentially regulate nuclear protein import
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2820426/
https://www.ncbi.nlm.nih.gov/pubmed/20016008
http://dx.doi.org/10.1091/mbc.E09-05-0374
work_keys_str_mv AT ogawayutaka twoisoformsofnpap60nup50differentiallyregulatenuclearproteinimport
AT miyamotoyoichi twoisoformsofnpap60nup50differentiallyregulatenuclearproteinimport
AT asallymunehiro twoisoformsofnpap60nup50differentiallyregulatenuclearproteinimport
AT okamasahiro twoisoformsofnpap60nup50differentiallyregulatenuclearproteinimport
AT yasudayoshinari twoisoformsofnpap60nup50differentiallyregulatenuclearproteinimport
AT yonedayoshihiro twoisoformsofnpap60nup50differentiallyregulatenuclearproteinimport