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Effect of nuclear localization and hydrodynamic delivery-induced cell division on phiC31 integrase activity
Phage φC31 integrase is a recombinase that can be expressed in mammalian cells to integrate plasmids carrying an attB sequence into the genome at specific pseudo attP locations. We demonstrate by immunofluoresence that wild-type φC31 integrase is cytoplasmic and that addition of a SV40 nuclear local...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2820593/ https://www.ncbi.nlm.nih.gov/pubmed/19847205 http://dx.doi.org/10.1038/gt.2009.136 |