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Role of Rho-kinase in mediating contraction of chicken embryo femoral arteries

Rho-kinase-dependent Ca(2+) sensitization is an essential process for contraction of mammalian vascular smooth muscle but the information about its effects in non-mammalian vessels is scarce. We aimed to investigate, using the Rho-kinase inhibitor hydroxyfasudil, the potential role of the Rho-kinase...

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Autores principales: Zoer, Bea, Blanco, Carlos E., Villamor, Eduardo
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2820664/
https://www.ncbi.nlm.nih.gov/pubmed/19936759
http://dx.doi.org/10.1007/s00360-009-0420-y
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author Zoer, Bea
Blanco, Carlos E.
Villamor, Eduardo
author_facet Zoer, Bea
Blanco, Carlos E.
Villamor, Eduardo
author_sort Zoer, Bea
collection PubMed
description Rho-kinase-dependent Ca(2+) sensitization is an essential process for contraction of mammalian vascular smooth muscle but the information about its effects in non-mammalian vessels is scarce. We aimed to investigate, using the Rho-kinase inhibitor hydroxyfasudil, the potential role of the Rho-kinase pathway of Ca(2+) sensitization in depolarization- and agonist-mediated contraction of chicken embryo (at day 19 of the 21 days of incubation) femoral arteries. Contraction elicited by KCl (125 mM) comprised two phases (phasic and tonic contraction), both of which were abolished in the absence of extracellular Ca(2+). Hydroxyfasudil (10 μM) left the initial phasic component nearly intact but abolished the tonic component. Hydroxyfasudil also induced a marked impairment of the contractions elicited by phenylephrine (PE), the thromboxane A(2) mimetic U46619, and endothelin-1. In contrast, inhibition of protein kinase C (PKC) by chelerythrine did not affect KCl- or PE-induced contractions, indicating lack of participation of PKC-mediated Ca(2+) sensitization. Incubation under chronic hypoxia (15% O(2) from day 0) impaired embryonic growth but did not significantly affect hydroxyfasudil-mediated relaxation. In summary, our findings are indicative of a role for Rho-kinase activity in depolarization- and agonist-induced force generation in chicken embryo femoral arteries.
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spelling pubmed-28206642010-02-19 Role of Rho-kinase in mediating contraction of chicken embryo femoral arteries Zoer, Bea Blanco, Carlos E. Villamor, Eduardo J Comp Physiol B Original Paper Rho-kinase-dependent Ca(2+) sensitization is an essential process for contraction of mammalian vascular smooth muscle but the information about its effects in non-mammalian vessels is scarce. We aimed to investigate, using the Rho-kinase inhibitor hydroxyfasudil, the potential role of the Rho-kinase pathway of Ca(2+) sensitization in depolarization- and agonist-mediated contraction of chicken embryo (at day 19 of the 21 days of incubation) femoral arteries. Contraction elicited by KCl (125 mM) comprised two phases (phasic and tonic contraction), both of which were abolished in the absence of extracellular Ca(2+). Hydroxyfasudil (10 μM) left the initial phasic component nearly intact but abolished the tonic component. Hydroxyfasudil also induced a marked impairment of the contractions elicited by phenylephrine (PE), the thromboxane A(2) mimetic U46619, and endothelin-1. In contrast, inhibition of protein kinase C (PKC) by chelerythrine did not affect KCl- or PE-induced contractions, indicating lack of participation of PKC-mediated Ca(2+) sensitization. Incubation under chronic hypoxia (15% O(2) from day 0) impaired embryonic growth but did not significantly affect hydroxyfasudil-mediated relaxation. In summary, our findings are indicative of a role for Rho-kinase activity in depolarization- and agonist-induced force generation in chicken embryo femoral arteries. Springer-Verlag 2009-11-20 2010 /pmc/articles/PMC2820664/ /pubmed/19936759 http://dx.doi.org/10.1007/s00360-009-0420-y Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Paper
Zoer, Bea
Blanco, Carlos E.
Villamor, Eduardo
Role of Rho-kinase in mediating contraction of chicken embryo femoral arteries
title Role of Rho-kinase in mediating contraction of chicken embryo femoral arteries
title_full Role of Rho-kinase in mediating contraction of chicken embryo femoral arteries
title_fullStr Role of Rho-kinase in mediating contraction of chicken embryo femoral arteries
title_full_unstemmed Role of Rho-kinase in mediating contraction of chicken embryo femoral arteries
title_short Role of Rho-kinase in mediating contraction of chicken embryo femoral arteries
title_sort role of rho-kinase in mediating contraction of chicken embryo femoral arteries
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2820664/
https://www.ncbi.nlm.nih.gov/pubmed/19936759
http://dx.doi.org/10.1007/s00360-009-0420-y
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