Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion

The host-guest complexation between an Alexa 488 labelled adamantane derivative and β-cyclodextrin is studied by Fluorescence Correlation Spectroscopy (FCS). A 1:1 complex stoichiometry and a high association equilibrium constant of K = 5.2 × 10(4) M(−1) are obtained in aqueous solution at 25 °C and...

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Autores principales: Granadero, Daniel, Bordello, Jorge, Pérez-Alvite, Maria Jesus, Novo, Mercedes, Al-Soufi, Wajih
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2820997/
https://www.ncbi.nlm.nih.gov/pubmed/20162009
http://dx.doi.org/10.3390/ijms11010173
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author Granadero, Daniel
Bordello, Jorge
Pérez-Alvite, Maria Jesus
Novo, Mercedes
Al-Soufi, Wajih
author_facet Granadero, Daniel
Bordello, Jorge
Pérez-Alvite, Maria Jesus
Novo, Mercedes
Al-Soufi, Wajih
author_sort Granadero, Daniel
collection PubMed
description The host-guest complexation between an Alexa 488 labelled adamantane derivative and β-cyclodextrin is studied by Fluorescence Correlation Spectroscopy (FCS). A 1:1 complex stoichiometry and a high association equilibrium constant of K = 5.2 × 10(4) M(−1) are obtained in aqueous solution at 25 °C and pH = 6. The necessary experimental conditions are discussed. FCS proves to be an excellent method for the determination of stoichiometry and association equilibrium constant of this type of complexes, where both host and guest are nonfluorescent and which are therefore not easily amenable to standard fluorescence spectroscopic methods.
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spelling pubmed-28209972010-02-16 Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion Granadero, Daniel Bordello, Jorge Pérez-Alvite, Maria Jesus Novo, Mercedes Al-Soufi, Wajih Int J Mol Sci Article The host-guest complexation between an Alexa 488 labelled adamantane derivative and β-cyclodextrin is studied by Fluorescence Correlation Spectroscopy (FCS). A 1:1 complex stoichiometry and a high association equilibrium constant of K = 5.2 × 10(4) M(−1) are obtained in aqueous solution at 25 °C and pH = 6. The necessary experimental conditions are discussed. FCS proves to be an excellent method for the determination of stoichiometry and association equilibrium constant of this type of complexes, where both host and guest are nonfluorescent and which are therefore not easily amenable to standard fluorescence spectroscopic methods. Molecular Diversity Preservation International (MDPI) 2010-01-12 /pmc/articles/PMC2820997/ /pubmed/20162009 http://dx.doi.org/10.3390/ijms11010173 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Granadero, Daniel
Bordello, Jorge
Pérez-Alvite, Maria Jesus
Novo, Mercedes
Al-Soufi, Wajih
Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion
title Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion
title_full Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion
title_fullStr Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion
title_full_unstemmed Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion
title_short Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion
title_sort host-guest complexation studied by fluorescence correlation spectroscopy: adamantane–cyclodextrin inclusion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2820997/
https://www.ncbi.nlm.nih.gov/pubmed/20162009
http://dx.doi.org/10.3390/ijms11010173
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AT bordellojorge hostguestcomplexationstudiedbyfluorescencecorrelationspectroscopyadamantanecyclodextrininclusion
AT perezalvitemariajesus hostguestcomplexationstudiedbyfluorescencecorrelationspectroscopyadamantanecyclodextrininclusion
AT novomercedes hostguestcomplexationstudiedbyfluorescencecorrelationspectroscopyadamantanecyclodextrininclusion
AT alsoufiwajih hostguestcomplexationstudiedbyfluorescencecorrelationspectroscopyadamantanecyclodextrininclusion

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