Photonic Methods to Enhance Fluorescence Correlation Spectroscopy and Single Molecule Fluorescence Detection

Recent advances in nanophotonics open the way for promising applications towards efficient single molecule fluorescence analysis. In this review, we discuss how photonic methods bring innovative solutions for two essential questions: how to detect a single molecule in a highly concentrated solution,...

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Detalles Bibliográficos
Autores principales: Wenger, Jérome, Rigneault, Hervé
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2820999/
https://www.ncbi.nlm.nih.gov/pubmed/20162011
http://dx.doi.org/10.3390/ijms11010206
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author Wenger, Jérome
Rigneault, Hervé
author_facet Wenger, Jérome
Rigneault, Hervé
author_sort Wenger, Jérome
collection PubMed
description Recent advances in nanophotonics open the way for promising applications towards efficient single molecule fluorescence analysis. In this review, we discuss how photonic methods bring innovative solutions for two essential questions: how to detect a single molecule in a highly concentrated solution, and how to enhance the faint optical signal emitted per molecule? The focus is set primarily on the widely used technique of fluorescence correlation spectroscopy (FCS), yet the discussion can be extended to other single molecule detection methods.
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spelling pubmed-28209992010-02-16 Photonic Methods to Enhance Fluorescence Correlation Spectroscopy and Single Molecule Fluorescence Detection Wenger, Jérome Rigneault, Hervé Int J Mol Sci Review Recent advances in nanophotonics open the way for promising applications towards efficient single molecule fluorescence analysis. In this review, we discuss how photonic methods bring innovative solutions for two essential questions: how to detect a single molecule in a highly concentrated solution, and how to enhance the faint optical signal emitted per molecule? The focus is set primarily on the widely used technique of fluorescence correlation spectroscopy (FCS), yet the discussion can be extended to other single molecule detection methods. Molecular Diversity Preservation International (MDPI) 2010-01-13 /pmc/articles/PMC2820999/ /pubmed/20162011 http://dx.doi.org/10.3390/ijms11010206 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Wenger, Jérome
Rigneault, Hervé
Photonic Methods to Enhance Fluorescence Correlation Spectroscopy and Single Molecule Fluorescence Detection
title Photonic Methods to Enhance Fluorescence Correlation Spectroscopy and Single Molecule Fluorescence Detection
title_full Photonic Methods to Enhance Fluorescence Correlation Spectroscopy and Single Molecule Fluorescence Detection
title_fullStr Photonic Methods to Enhance Fluorescence Correlation Spectroscopy and Single Molecule Fluorescence Detection
title_full_unstemmed Photonic Methods to Enhance Fluorescence Correlation Spectroscopy and Single Molecule Fluorescence Detection
title_short Photonic Methods to Enhance Fluorescence Correlation Spectroscopy and Single Molecule Fluorescence Detection
title_sort photonic methods to enhance fluorescence correlation spectroscopy and single molecule fluorescence detection
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2820999/
https://www.ncbi.nlm.nih.gov/pubmed/20162011
http://dx.doi.org/10.3390/ijms11010206
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AT rigneaultherve photonicmethodstoenhancefluorescencecorrelationspectroscopyandsinglemoleculefluorescencedetection

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