Melatonin and Structurally-Related Compounds Protect Synaptosomal Membranes from Free Radical Damage

Since biological membranes are composed of lipids and proteins we tested the in vitro antioxidant properties of several indoleamines from the tryptophan metabolic pathway in the pineal gland against oxidative damage to lipids and proteins of synaptosomes isolated from the rat brain. Free radicals we...

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Detalles Bibliográficos
Autores principales: Millán-Plano, Sergio, Piedrafita, Eduardo, Miana-Mena, Francisco J., Fuentes-Broto, Lorena, Martínez-Ballarín, Enrique, López-Pingarrón, Laura, Sáenz, María A., García, Joaquín J.
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2821006/
https://www.ncbi.nlm.nih.gov/pubmed/20162018
http://dx.doi.org/10.3390/ijms11010312
Descripción
Sumario:Since biological membranes are composed of lipids and proteins we tested the in vitro antioxidant properties of several indoleamines from the tryptophan metabolic pathway in the pineal gland against oxidative damage to lipids and proteins of synaptosomes isolated from the rat brain. Free radicals were generated by incubation with 0.1 mM FeCl(3), and 0.1 mM ascorbic acid. Levels of malondialdehyde (MDA) plus 4-hydroxyalkenal (4-HDA), and carbonyl content in the proteins were measured as indices of oxidative damage to lipids and proteins, respectively. Pinoline was the most powerful antioxidant evaluated, with melatonin, N-acetylserotonin, 5-hydroxytryptophan, 5-methoxytryptamine, 5-methoxytryptophol, and tryptoline also acting as antioxidants.

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