Cargando…
FoxM1, a Forkhead Transcription Factor Is a Master Cell Cycle Regulator for Mouse Mature T Cells but Not Double Positive Thymocytes
FoxM1 is a forkhead box transcription factor and a known master regulator required for different phases of the cell cycle. In cell lines, FoxM1 deficient cells exhibit delayed S phase entry, aneuploidy, polyploidy and can't complete mitosis. In vivo, FoxM1 is expressed mostly in proliferating c...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2821927/ https://www.ncbi.nlm.nih.gov/pubmed/20169079 http://dx.doi.org/10.1371/journal.pone.0009229 |
_version_ | 1782177478470533120 |
---|---|
author | Xue, Ling Chiang, Leslie He, Bo Zhao, You-Yang Winoto, Astar |
author_facet | Xue, Ling Chiang, Leslie He, Bo Zhao, You-Yang Winoto, Astar |
author_sort | Xue, Ling |
collection | PubMed |
description | FoxM1 is a forkhead box transcription factor and a known master regulator required for different phases of the cell cycle. In cell lines, FoxM1 deficient cells exhibit delayed S phase entry, aneuploidy, polyploidy and can't complete mitosis. In vivo, FoxM1 is expressed mostly in proliferating cells but is surprisingly also found in non-proliferating CD4(+)CD8(+) double positive thymocytes. Here, we addressed the role of FoxM1 in T cell development by generating and analyzing two different lines of T-cell specific FoxM1 deficient mice. As expected, FoxM1 is required for proliferation of early thymocytes and activated mature T cells. Defective expression of many cell cycle proteins was detected, including cyclin A, cyclin B1, cdc2, cdk2, p27 and the Rb family members p107 and p130 but surprisingly not survivin. Unexpectedly, loss of FoxM1 only affects a few cell cycle proteins in CD4(+)CD8(+) thymocytes and has little effect on their sensitivity to apoptosis and the subsequent steps of T cell differentiation. Thus, regulation of cell cycle genes by FoxM1 is stage- and context-dependent. |
format | Text |
id | pubmed-2821927 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28219272010-02-19 FoxM1, a Forkhead Transcription Factor Is a Master Cell Cycle Regulator for Mouse Mature T Cells but Not Double Positive Thymocytes Xue, Ling Chiang, Leslie He, Bo Zhao, You-Yang Winoto, Astar PLoS One Research Article FoxM1 is a forkhead box transcription factor and a known master regulator required for different phases of the cell cycle. In cell lines, FoxM1 deficient cells exhibit delayed S phase entry, aneuploidy, polyploidy and can't complete mitosis. In vivo, FoxM1 is expressed mostly in proliferating cells but is surprisingly also found in non-proliferating CD4(+)CD8(+) double positive thymocytes. Here, we addressed the role of FoxM1 in T cell development by generating and analyzing two different lines of T-cell specific FoxM1 deficient mice. As expected, FoxM1 is required for proliferation of early thymocytes and activated mature T cells. Defective expression of many cell cycle proteins was detected, including cyclin A, cyclin B1, cdc2, cdk2, p27 and the Rb family members p107 and p130 but surprisingly not survivin. Unexpectedly, loss of FoxM1 only affects a few cell cycle proteins in CD4(+)CD8(+) thymocytes and has little effect on their sensitivity to apoptosis and the subsequent steps of T cell differentiation. Thus, regulation of cell cycle genes by FoxM1 is stage- and context-dependent. Public Library of Science 2010-02-16 /pmc/articles/PMC2821927/ /pubmed/20169079 http://dx.doi.org/10.1371/journal.pone.0009229 Text en Xue et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Xue, Ling Chiang, Leslie He, Bo Zhao, You-Yang Winoto, Astar FoxM1, a Forkhead Transcription Factor Is a Master Cell Cycle Regulator for Mouse Mature T Cells but Not Double Positive Thymocytes |
title | FoxM1, a Forkhead Transcription Factor Is a Master Cell Cycle Regulator for Mouse Mature T Cells but Not Double Positive Thymocytes |
title_full | FoxM1, a Forkhead Transcription Factor Is a Master Cell Cycle Regulator for Mouse Mature T Cells but Not Double Positive Thymocytes |
title_fullStr | FoxM1, a Forkhead Transcription Factor Is a Master Cell Cycle Regulator for Mouse Mature T Cells but Not Double Positive Thymocytes |
title_full_unstemmed | FoxM1, a Forkhead Transcription Factor Is a Master Cell Cycle Regulator for Mouse Mature T Cells but Not Double Positive Thymocytes |
title_short | FoxM1, a Forkhead Transcription Factor Is a Master Cell Cycle Regulator for Mouse Mature T Cells but Not Double Positive Thymocytes |
title_sort | foxm1, a forkhead transcription factor is a master cell cycle regulator for mouse mature t cells but not double positive thymocytes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2821927/ https://www.ncbi.nlm.nih.gov/pubmed/20169079 http://dx.doi.org/10.1371/journal.pone.0009229 |
work_keys_str_mv | AT xueling foxm1aforkheadtranscriptionfactorisamastercellcycleregulatorformousematuretcellsbutnotdoublepositivethymocytes AT chiangleslie foxm1aforkheadtranscriptionfactorisamastercellcycleregulatorformousematuretcellsbutnotdoublepositivethymocytes AT hebo foxm1aforkheadtranscriptionfactorisamastercellcycleregulatorformousematuretcellsbutnotdoublepositivethymocytes AT zhaoyouyang foxm1aforkheadtranscriptionfactorisamastercellcycleregulatorformousematuretcellsbutnotdoublepositivethymocytes AT winotoastar foxm1aforkheadtranscriptionfactorisamastercellcycleregulatorformousematuretcellsbutnotdoublepositivethymocytes |