Carbon metabolism-mediated myogenic differentiation

The role of nutrients and metabolism in cellular differentiation is poorly understood. Using RNAi screening, metabolic profiling and small-molecule probes, we discovered three metabolic enzymes whose knockdown induces differentiation of mouse C2C12 myoblasts even in the presence mitogens: phosphogly...

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Detalles Bibliográficos
Autores principales: Bracha, Abigail L., Ramanathan, Arvind, Huang, Sui, Ingber, Donald E., Schreiber, Stuart L.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2822028/
https://www.ncbi.nlm.nih.gov/pubmed/20081855
http://dx.doi.org/10.1038/nchembio.301
Descripción
Sumario:The role of nutrients and metabolism in cellular differentiation is poorly understood. Using RNAi screening, metabolic profiling and small-molecule probes, we discovered three metabolic enzymes whose knockdown induces differentiation of mouse C2C12 myoblasts even in the presence mitogens: phosphoglycerate kinase (Pgk1), hexose-6-phosphate dehydrogenase (H6pd) and ATP citrate lyase (Acl). These enzymes and the pathways they regulate provide novel targets for the control of myogenic differentiation in myoblasts and rhabdomyosarcoma cells.

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