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p73 is essential for vitamin D mediated osteoblastic differentiation
The secosteroid hormone vitamin D3 (VD3) exerts its biological actions through its cognate receptor, the Vitamin D Receptor (VDR). Vitamin D3 and VDR play a key role in bone formation and keratinocyte differentiation, exert anti-proliferative actions in human cancer, and is widely used as a chemothe...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2822032/ https://www.ncbi.nlm.nih.gov/pubmed/19779497 http://dx.doi.org/10.1038/cdd.2009.135 |
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author | Kommagani, Ramakrishna Whitlatch, Andrew Leonard, Mary K. Kadakia, Madhavi P. |
author_facet | Kommagani, Ramakrishna Whitlatch, Andrew Leonard, Mary K. Kadakia, Madhavi P. |
author_sort | Kommagani, Ramakrishna |
collection | PubMed |
description | The secosteroid hormone vitamin D3 (VD3) exerts its biological actions through its cognate receptor, the Vitamin D Receptor (VDR). Vitamin D3 and VDR play a key role in bone formation and keratinocyte differentiation, exert anti-proliferative actions in human cancer, and is widely used as a chemotherapeutic agent for cancer. Additionally, VD3 promotes differentiation of human osteosarcoma cells by up-regulating genes involved in cell cycle arrest and osteoblastic differentiation. Although considerable work has been carried out in understanding the molecular mechanisms underlying the VD3-mediated differentiation of human osteosarcoma cells, the upstream regulation of VD3 signaling pathway is still unclear. In this study we demonstrate that p73 acts as an upstream regulator of VD3 mediated osteoblastic differentiation. Transcription factor p73, a p53 homolog has been shown to play a role in development and recently been termed as a tumor suppressor. Silencing p73 results in a significant reduction of VD3 mediated osteoblastic differentiation; while DNA damage induced p73 leads to an increase in VD3 mediated differentiation of osteosarcoma cells. Together, our data implicate a novel role for p73 in vitamin D mediated differentiation of human osteosarcoma cells. |
format | Text |
id | pubmed-2822032 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
record_format | MEDLINE/PubMed |
spelling | pubmed-28220322010-09-01 p73 is essential for vitamin D mediated osteoblastic differentiation Kommagani, Ramakrishna Whitlatch, Andrew Leonard, Mary K. Kadakia, Madhavi P. Cell Death Differ Article The secosteroid hormone vitamin D3 (VD3) exerts its biological actions through its cognate receptor, the Vitamin D Receptor (VDR). Vitamin D3 and VDR play a key role in bone formation and keratinocyte differentiation, exert anti-proliferative actions in human cancer, and is widely used as a chemotherapeutic agent for cancer. Additionally, VD3 promotes differentiation of human osteosarcoma cells by up-regulating genes involved in cell cycle arrest and osteoblastic differentiation. Although considerable work has been carried out in understanding the molecular mechanisms underlying the VD3-mediated differentiation of human osteosarcoma cells, the upstream regulation of VD3 signaling pathway is still unclear. In this study we demonstrate that p73 acts as an upstream regulator of VD3 mediated osteoblastic differentiation. Transcription factor p73, a p53 homolog has been shown to play a role in development and recently been termed as a tumor suppressor. Silencing p73 results in a significant reduction of VD3 mediated osteoblastic differentiation; while DNA damage induced p73 leads to an increase in VD3 mediated differentiation of osteosarcoma cells. Together, our data implicate a novel role for p73 in vitamin D mediated differentiation of human osteosarcoma cells. 2009-09-25 2010-03 /pmc/articles/PMC2822032/ /pubmed/19779497 http://dx.doi.org/10.1038/cdd.2009.135 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Kommagani, Ramakrishna Whitlatch, Andrew Leonard, Mary K. Kadakia, Madhavi P. p73 is essential for vitamin D mediated osteoblastic differentiation |
title | p73 is essential for vitamin D mediated osteoblastic differentiation |
title_full | p73 is essential for vitamin D mediated osteoblastic differentiation |
title_fullStr | p73 is essential for vitamin D mediated osteoblastic differentiation |
title_full_unstemmed | p73 is essential for vitamin D mediated osteoblastic differentiation |
title_short | p73 is essential for vitamin D mediated osteoblastic differentiation |
title_sort | p73 is essential for vitamin d mediated osteoblastic differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2822032/ https://www.ncbi.nlm.nih.gov/pubmed/19779497 http://dx.doi.org/10.1038/cdd.2009.135 |
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