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Capture and Release of Alkyne-Derivatized Glycerophospholipids Using Cobalt Chemistry
Alkyne modified phospholipids can be unambiguously identified and differentiated from native species in complex mixtures by formation of dicobalthexacarbonyl complexes. This reaction is specific for alkynes and is unaffected by other glycerophospholipid related moieties. Enrichment of cells with alk...
| Autores principales: | , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2822082/ https://www.ncbi.nlm.nih.gov/pubmed/20098428 http://dx.doi.org/10.1038/nchembio.311 |
| _version_ | 1782177482423664640 |
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| author | Milne, Stephen B. Tallman, Keri A. Serwa, Remigiusz Rouzer, Carol A. Armstrong, Michelle D. Marnett, Lawrence J. Lukehart, Charles M. Porter, Ned A. Brown, H. Alex |
| author_facet | Milne, Stephen B. Tallman, Keri A. Serwa, Remigiusz Rouzer, Carol A. Armstrong, Michelle D. Marnett, Lawrence J. Lukehart, Charles M. Porter, Ned A. Brown, H. Alex |
| author_sort | Milne, Stephen B. |
| collection | PubMed |
| description | Alkyne modified phospholipids can be unambiguously identified and differentiated from native species in complex mixtures by formation of dicobalthexacarbonyl complexes. This reaction is specific for alkynes and is unaffected by other glycerophospholipid related moieties. Enrichment of cells with alkyne-derivatized fatty acids or glycerophospholipids followed by solid phase sequestration and release is a promising new method for unequivocally monitoring individual glycerophospholipids following incorporation and facilitates lipidomic analysis of substrates and products. |
| format | Text |
| id | pubmed-2822082 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2010 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-28220822010-09-01 Capture and Release of Alkyne-Derivatized Glycerophospholipids Using Cobalt Chemistry Milne, Stephen B. Tallman, Keri A. Serwa, Remigiusz Rouzer, Carol A. Armstrong, Michelle D. Marnett, Lawrence J. Lukehart, Charles M. Porter, Ned A. Brown, H. Alex Nat Chem Biol Article Alkyne modified phospholipids can be unambiguously identified and differentiated from native species in complex mixtures by formation of dicobalthexacarbonyl complexes. This reaction is specific for alkynes and is unaffected by other glycerophospholipid related moieties. Enrichment of cells with alkyne-derivatized fatty acids or glycerophospholipids followed by solid phase sequestration and release is a promising new method for unequivocally monitoring individual glycerophospholipids following incorporation and facilitates lipidomic analysis of substrates and products. 2010-01-24 2010-03 /pmc/articles/PMC2822082/ /pubmed/20098428 http://dx.doi.org/10.1038/nchembio.311 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Milne, Stephen B. Tallman, Keri A. Serwa, Remigiusz Rouzer, Carol A. Armstrong, Michelle D. Marnett, Lawrence J. Lukehart, Charles M. Porter, Ned A. Brown, H. Alex Capture and Release of Alkyne-Derivatized Glycerophospholipids Using Cobalt Chemistry |
| title | Capture and Release of Alkyne-Derivatized Glycerophospholipids Using Cobalt Chemistry |
| title_full | Capture and Release of Alkyne-Derivatized Glycerophospholipids Using Cobalt Chemistry |
| title_fullStr | Capture and Release of Alkyne-Derivatized Glycerophospholipids Using Cobalt Chemistry |
| title_full_unstemmed | Capture and Release of Alkyne-Derivatized Glycerophospholipids Using Cobalt Chemistry |
| title_short | Capture and Release of Alkyne-Derivatized Glycerophospholipids Using Cobalt Chemistry |
| title_sort | capture and release of alkyne-derivatized glycerophospholipids using cobalt chemistry |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2822082/ https://www.ncbi.nlm.nih.gov/pubmed/20098428 http://dx.doi.org/10.1038/nchembio.311 |
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