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The effect of glucocorticoids on tendon cell viability in human tendon explants

Background and purpose Previous studies on the culture of human tenocytes have shown that dexamethasone and triamcino-lone reduce cell viability, suppress cell proliferation, and reduce collagen synthesis. However, such cell cultures lack the extracellular matrix and three-dimensional structure of n...

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Autores principales: Wan Nar Wong, Margaret, Lui, Wai Ting, Chuen Fu, Sai, Man Lee, Kwong
Formato: Texto
Lenguaje:English
Publicado: Informa Healthcare 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823211/
https://www.ncbi.nlm.nih.gov/pubmed/19421908
http://dx.doi.org/10.3109/17453670902988386
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author Wan Nar Wong, Margaret
Lui, Wai Ting
Chuen Fu, Sai
Man Lee, Kwong
author_facet Wan Nar Wong, Margaret
Lui, Wai Ting
Chuen Fu, Sai
Man Lee, Kwong
author_sort Wan Nar Wong, Margaret
collection PubMed
description Background and purpose Previous studies on the culture of human tenocytes have shown that dexamethasone and triamcino-lone reduce cell viability, suppress cell proliferation, and reduce collagen synthesis. However, such cell cultures lack the extracellular matrix and three-dimensional structure of normal tendons, which affects their response to stimuli. We established a human tendon explant culture system and tested the effects of dexamethasone and triamcinolone on cell viability. Methods Primary human tendon explant cultures were prepared from healthy hamstring tendons. Tendon strips were harvested from hamstring tendons and cultured in 24-well plates in Dulbecco’s modification of Eagle’s Medium (DMEM) supplemented with 2% fetal calf serum. The tendon explants were treated with 0 μM (control), 10 μM, or 100 μM dexamethasone sodium phosphate or 0 μM (control), 10 μM, or 100 μM triamcinolone acetonide in DMEM for 96 h. Cell viability was measured by Alamar blue assay before and after glucocorticoid treatment. Results Incubation with 10 μM and 100 μM dexamethasone reduced cell viability in human tendon explants by 35% and 45%, respectively, as compared to a 6% increase in the controls (p = 0.01, mixed-effects ANOVA). Triamcinolone at 10 μM and 100 μM reduced cell viability by 33% and 36%, respectively, as compared to a 9% increase in the controls (p = 0.07, mixed-effects ANOVA). Interpretation Human tendon explant cultures can be used to study the effects of glucocorticoids on human tendon. Dexamethasone and triamcinolone suppress the cell viability of human tendon in its natural 3-dimensional environment with matrix anchorage. Human tendon explant cultures provide a species-specific model for further investigation of the effects of glucocorticoids on the metabolism of the extracellular matrix of human tendon, and on its mechanical properties.
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spelling pubmed-28232112010-02-18 The effect of glucocorticoids on tendon cell viability in human tendon explants Wan Nar Wong, Margaret Lui, Wai Ting Chuen Fu, Sai Man Lee, Kwong Acta Orthop Research Article Background and purpose Previous studies on the culture of human tenocytes have shown that dexamethasone and triamcino-lone reduce cell viability, suppress cell proliferation, and reduce collagen synthesis. However, such cell cultures lack the extracellular matrix and three-dimensional structure of normal tendons, which affects their response to stimuli. We established a human tendon explant culture system and tested the effects of dexamethasone and triamcinolone on cell viability. Methods Primary human tendon explant cultures were prepared from healthy hamstring tendons. Tendon strips were harvested from hamstring tendons and cultured in 24-well plates in Dulbecco’s modification of Eagle’s Medium (DMEM) supplemented with 2% fetal calf serum. The tendon explants were treated with 0 μM (control), 10 μM, or 100 μM dexamethasone sodium phosphate or 0 μM (control), 10 μM, or 100 μM triamcinolone acetonide in DMEM for 96 h. Cell viability was measured by Alamar blue assay before and after glucocorticoid treatment. Results Incubation with 10 μM and 100 μM dexamethasone reduced cell viability in human tendon explants by 35% and 45%, respectively, as compared to a 6% increase in the controls (p = 0.01, mixed-effects ANOVA). Triamcinolone at 10 μM and 100 μM reduced cell viability by 33% and 36%, respectively, as compared to a 9% increase in the controls (p = 0.07, mixed-effects ANOVA). Interpretation Human tendon explant cultures can be used to study the effects of glucocorticoids on human tendon. Dexamethasone and triamcinolone suppress the cell viability of human tendon in its natural 3-dimensional environment with matrix anchorage. Human tendon explant cultures provide a species-specific model for further investigation of the effects of glucocorticoids on the metabolism of the extracellular matrix of human tendon, and on its mechanical properties. Informa Healthcare 2009-06-05 2009-06-01 /pmc/articles/PMC2823211/ /pubmed/19421908 http://dx.doi.org/10.3109/17453670902988386 Text en Copyright: © Nordic Orthopedic Federation http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the source is credited.
spellingShingle Research Article
Wan Nar Wong, Margaret
Lui, Wai Ting
Chuen Fu, Sai
Man Lee, Kwong
The effect of glucocorticoids on tendon cell viability in human tendon explants
title The effect of glucocorticoids on tendon cell viability in human tendon explants
title_full The effect of glucocorticoids on tendon cell viability in human tendon explants
title_fullStr The effect of glucocorticoids on tendon cell viability in human tendon explants
title_full_unstemmed The effect of glucocorticoids on tendon cell viability in human tendon explants
title_short The effect of glucocorticoids on tendon cell viability in human tendon explants
title_sort effect of glucocorticoids on tendon cell viability in human tendon explants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823211/
https://www.ncbi.nlm.nih.gov/pubmed/19421908
http://dx.doi.org/10.3109/17453670902988386
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