A novel FRET pair for detection of parallel DNA triplexes by the LightCycler

BACKGROUND: Melting temperature of DNA structures can be determined on the LightCycler using quenching of FAM. This method is very suitable for pH independent melting point (Tm) determination performed at basic or neutral pH, as a high throughput alternative to UV absorbance measurements. At acidic...

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Detalles Bibliográficos
Autores principales: Schneider, Uffe V, Severinsen, Jette K, Géci, Imrich, Okkels, Limei M, Jøhnk, Nina, Mikkelsen, Nikolaj D, Klinge, Teena, Pedersen, Erik B, Westh, Henrik, Lisby, Gorm
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823659/
https://www.ncbi.nlm.nih.gov/pubmed/20102641
http://dx.doi.org/10.1186/1472-6750-10-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823659/
https://www.ncbi.nlm.nih.gov/pubmed/20102641
http://dx.doi.org/10.1186/1472-6750-10-4

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