Cargando…

A novel FRET pair for detection of parallel DNA triplexes by the LightCycler

BACKGROUND: Melting temperature of DNA structures can be determined on the LightCycler using quenching of FAM. This method is very suitable for pH independent melting point (Tm) determination performed at basic or neutral pH, as a high throughput alternative to UV absorbance measurements. At acidic...

Descripción completa

Detalles Bibliográficos
Autores principales:	Schneider, Uffe V, Severinsen, Jette K, Géci, Imrich, Okkels, Limei M, Jøhnk, Nina, Mikkelsen, Nikolaj D, Klinge, Teena, Pedersen, Erik B, Westh, Henrik, Lisby, Gorm
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823659/ https://www.ncbi.nlm.nih.gov/pubmed/20102641 http://dx.doi.org/10.1186/1472-6750-10-4

Ejemplares similares

Optimal design of parallel triplex forming oligonucleotides containing Twisted Intercalating Nucleic Acids—TINA
por: Schneider, Uffe V., et al.
Publicado: (2010)

Increasing the Analytical Sensitivity by Oligonucleotides Modified with Para- and Ortho-Twisted Intercalating Nucleic Acids – TINA
por: Schneider, Uffe V., et al.
Publicado: (2011)

Improved Efficiency and Robustness in qPCR and Multiplex End-Point PCR by Twisted Intercalating Nucleic Acid Modified Primers
por: Schneider, Uffe Vest, et al.
Publicado: (2012)

LightCycler SeptiFast in early diagnosis of sepsis: our experience
por: Raineri, SM, et al.
Publicado: (2009)

Variability and cost implications of three generations of the Roche LightCycler® 480
por: Dullaert-de Boer, Maria, et al.
Publicado: (2018)

Correction: Variability and cost implications of three generations of the Roche LightCycler® 480
por: Dullaert-de Boer, Maria, et al.
Publicado: (2018)

Analytical Performance of the Roche LightCycler® Mycobacterium Detection Kit for the Diagnosis of Clinically Important Mycobacterial Species
por: Omar, Shaheed V., et al.
Publicado: (2011)

LightCycler SeptiFast assay as a tool for the rapid diagnosis of sepsis in patients receiving antimicrobial therapy
por: Vince, Adriana, et al.
Publicado: (2008)

LightCycler SeptiFast technology in patients with solid malignancies: clinical utility for rapid etiologic diagnosis of sepsis
por: Dubská, Lenka, et al.
Publicado: (2012)

Detection and subtyping of Herpes simplex virus in clinical samples by LightCycler PCR, enzyme immunoassay and cell culture
por: Burrows, Julie, et al.
Publicado: (2002)

A real-time PCR assay for the detection and quantitation of Campylobacter jejuni using SYBR Green I and the LightCycler.
por: Yang, Chengbo, et al.
Publicado: (2004)

Accuracy of LightCycler® SeptiFast for the detection and identification of pathogens in the blood of patients with suspected sepsis: a systematic review protocol
por: Dark, Paul, et al.
Publicado: (2012)

Methicillin-resistant Staphylococcus aureus in Nasal Surveillance Swabs at an Intensive Care Unit: An Evaluation of the LightCycler MRSA Advanced Test
por: Huh, Hee Jin, et al.
Publicado: (2012)

Monkeypox virus detection in rodents using real-time 3′-minor groove binder TaqMan® assays on the Roche LightCycler
por: Kulesh, David A, et al.
Publicado: (2004)

Rapid Genotyping of the Human Renin (REN) Gene by the LightCycler® Instrument: Identification of Unexpected Nucleotide Substitutions within the Selected Hybridization Probe Area
por: Wee, Line, et al.
Publicado: (2010)

Human immunodeficiency virus type 1 (HIV-1) proviral DNA load in purified CD4+ cells by LightCycler(® )Real-time PCR
por: Kabamba-Mukadi, Benoît, et al.
Publicado: (2005)

Rapid identification of Bordetella pertussis pertactin gene variants using LightCycler real-time polymerase chain reaction combined with melting curve analysis and gel electrophoresis.
por: Mäkinen, J, et al.
Publicado: (2001)

Evaluation of Type-Specific Real-Time PCR Assays Using the LightCycler and J.B.A.I.D.S. for Detection of Adenoviruses in Species HAdV-C
por: Jones, Morris S., et al.
Publicado: (2011)

Nonaplex PCR using Cliffhanger primers to identify diarrhoeagenic Escherichia coli from crude lysates of human faecal samples
por: Schneider, Uffe Vest, et al.
Publicado: (2018)

Triplexed CEA-NSE-PSA Immunoassay Using Time-Gated Terbium-to-Quantum Dot FRET
por: Bhuckory, Shashi, et al.
Publicado: (2020)

Point of care testing for infectious disease: ownership and quality
por: Lisby, Jan Gorm, et al.
Publicado: (2021)

Automated cyclers used in peritoneal dialysis: technical aspects for the clinician
por: Chaudhry, Rafia I, et al.
Publicado: (2015)

Parallel G-triplexes and G-hairpins as potential transitory ensembles in the folding of parallel-stranded DNA G-Quadruplexes
por: Stadlbauer, Petr, et al.
Publicado: (2019)

Coronaviruses in brain tissue from patients with multiple sclerosis
por: Dessau, Ram B., et al.
Publicado: (2001)

CLASSIC ULTRA- RAPID CYCLER BIPOLAR DISORDER : A CASE REPORT
por: Garg, P.D., et al.
Publicado: (1998)

A Rapid Digital PCR System with a Pressurized Thermal Cycler
por: Chen, Xuee, et al.
Publicado: (2021)

PEAKS AND VALLEYS: BIPOLAR DISORDER, RAPID CYCLERS AND ENERGY DRINKS CONSUMPTION
por: Calvo Valcárcel, M., et al.
Publicado: (2023)

Parallel triplex structure formed between stretched single-stranded DNA and homologous duplex DNA
por: Chen, Jin, et al.
Publicado: (2017)

A Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings
por: Wong, Grace, et al.
Publicado: (2015)

Evaluation of the Light-Cycler® SeptiFast Test in Newborns With Suspicion of Nosocomial Sepsis
por: Ortiz Ibarra, Javier, et al.
Publicado: (2015)

A Rapid and Low-Cost PCR Thermal Cycler for Infectious Disease Diagnostics
por: Chan, Kamfai, et al.
Publicado: (2016)

Fluorogenic thiazole orange TOTFO probes stabilise parallel DNA triplexes at pH 7 and above
por: Walsh, Sarah, et al.
Publicado: (2018)

Detection of Ochratoxin a Using Molecular Beacons and Real-Time PCR Thermal Cycler
por: Sanzani, Simona Marianna, et al.
Publicado: (2015)

CYCLeR—a novel tool for the full isoform assembly and quantification of circRNAs
por: Stefanov, Stefan R, et al.
Publicado: (2022)

Smartphone-operated affordable PCR thermal cycler for the detection of antimicrobial resistant bacterial genes
por: Pudasaini, Sanam, et al.
Publicado: (2023)

Antimalarial NADPH-Consuming Redox-Cyclers As Superior Glucose-6-Phosphate Dehydrogenase Deficiency Copycats
por: Bielitza, Max, et al.
Publicado: (2015)

The Rotary Zone Thermal Cycler: A Low-Power System Enabling Automated Rapid PCR
por: Bartsch, Michael S., et al.
Publicado: (2015)

Comparing the effect of peritoneal dialysis cycler type on patient-reported satisfaction, support needs and treatments
por: Shamy, Osama El, et al.
Publicado: (2022)

Multicenter evaluation of the QIAstat Respiratory Panel—A new rapid highly multiplexed PCR based assay for diagnosis of acute respiratory tract infections
por: Parčina, Marijo, et al.
Publicado: (2020)

An Agreement of Antigen Tests on Oral Pharyngeal Swabs or Less Invasive Testing With Reverse Transcription Polymerase Chain Reaction for Detecting SARS-CoV-2 in Adults: Protocol for a Prospective Nationwide Observational Study
por: Schneider, Uffe Vest, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_6mdg5d29fr1lroqj0omkr2gn3f