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Association of LEC and tnpA Helicobacter pylori genes with gastric cancer in a Brazilian population

BACKGROUND: H. pylori seroprevalence in Brazilians varies and is dependent on socioeconomic status, sanitation conditions and ethnicity; furthermore, H. pylori is not always associated with the incidence of gastric cancer, suggesting the role of more virulent strains. The purpose of this study was t...

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Autores principales: Mattar, Rejane, Monteiro, Maria S, Marques, Sergio B, Zilberstein, Bruno, Hashimoto, Cláudio L, Carrilho, Flair J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823720/
https://www.ncbi.nlm.nih.gov/pubmed/20205796
http://dx.doi.org/10.1186/1750-9378-5-1
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author Mattar, Rejane
Monteiro, Maria S
Marques, Sergio B
Zilberstein, Bruno
Hashimoto, Cláudio L
Carrilho, Flair J
author_facet Mattar, Rejane
Monteiro, Maria S
Marques, Sergio B
Zilberstein, Bruno
Hashimoto, Cláudio L
Carrilho, Flair J
author_sort Mattar, Rejane
collection PubMed
description BACKGROUND: H. pylori seroprevalence in Brazilians varies and is dependent on socioeconomic status, sanitation conditions and ethnicity; furthermore, H. pylori is not always associated with the incidence of gastric cancer, suggesting the role of more virulent strains. The purpose of this study was to analyze the association of more virulent H. pylori strains with gastric cancer. METHODS: DNA was extracted from gastric biopsies of thirty-four cases of gastric cancer (11 intestinal-type, 23 diffuse-type), and thirty-four of patients with endoscopic gastritis. The presence of cagPAI genes (cagA, cagA promoter, cagE, cagM, tnpB, tnpA, cagT and the left end of the cagII (LEC)) and babA were analyzed by PCR. RESULTS: Comparison of H. pylori isolates from gastric cancer and gastritis patients showed significant associations of tnpA and LEC with gastric cancer (73.5% [OR, 6.66; 95% CI, 2.30-19.25] and 58.8% [OR, 10.71; 95% CI, 3.07-37.28] of cases, respectively). Other cagPAI genes were detected in both groups at similar frequencies. CONCLUSIONS: tnpA and LEC of H. pylori cagPAI were associated with gastric cancer; nonetheless, these results were restricted within this group of patients and further studies are needed to confirm these results in a larger sample and determine their role in gastric carcinogenesis.
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spelling pubmed-28237202010-02-18 Association of LEC and tnpA Helicobacter pylori genes with gastric cancer in a Brazilian population Mattar, Rejane Monteiro, Maria S Marques, Sergio B Zilberstein, Bruno Hashimoto, Cláudio L Carrilho, Flair J Infect Agent Cancer Short Report BACKGROUND: H. pylori seroprevalence in Brazilians varies and is dependent on socioeconomic status, sanitation conditions and ethnicity; furthermore, H. pylori is not always associated with the incidence of gastric cancer, suggesting the role of more virulent strains. The purpose of this study was to analyze the association of more virulent H. pylori strains with gastric cancer. METHODS: DNA was extracted from gastric biopsies of thirty-four cases of gastric cancer (11 intestinal-type, 23 diffuse-type), and thirty-four of patients with endoscopic gastritis. The presence of cagPAI genes (cagA, cagA promoter, cagE, cagM, tnpB, tnpA, cagT and the left end of the cagII (LEC)) and babA were analyzed by PCR. RESULTS: Comparison of H. pylori isolates from gastric cancer and gastritis patients showed significant associations of tnpA and LEC with gastric cancer (73.5% [OR, 6.66; 95% CI, 2.30-19.25] and 58.8% [OR, 10.71; 95% CI, 3.07-37.28] of cases, respectively). Other cagPAI genes were detected in both groups at similar frequencies. CONCLUSIONS: tnpA and LEC of H. pylori cagPAI were associated with gastric cancer; nonetheless, these results were restricted within this group of patients and further studies are needed to confirm these results in a larger sample and determine their role in gastric carcinogenesis. BioMed Central 2010-01-11 /pmc/articles/PMC2823720/ /pubmed/20205796 http://dx.doi.org/10.1186/1750-9378-5-1 Text en Copyright ©2010 Mattar et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Mattar, Rejane
Monteiro, Maria S
Marques, Sergio B
Zilberstein, Bruno
Hashimoto, Cláudio L
Carrilho, Flair J
Association of LEC and tnpA Helicobacter pylori genes with gastric cancer in a Brazilian population
title Association of LEC and tnpA Helicobacter pylori genes with gastric cancer in a Brazilian population
title_full Association of LEC and tnpA Helicobacter pylori genes with gastric cancer in a Brazilian population
title_fullStr Association of LEC and tnpA Helicobacter pylori genes with gastric cancer in a Brazilian population
title_full_unstemmed Association of LEC and tnpA Helicobacter pylori genes with gastric cancer in a Brazilian population
title_short Association of LEC and tnpA Helicobacter pylori genes with gastric cancer in a Brazilian population
title_sort association of lec and tnpa helicobacter pylori genes with gastric cancer in a brazilian population
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823720/
https://www.ncbi.nlm.nih.gov/pubmed/20205796
http://dx.doi.org/10.1186/1750-9378-5-1
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