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Serotype- and strain- dependent contribution of the sensor kinase CovS of the CovRS two-component system to Streptococcus pyogenes pathogenesis

BACKGROUND: The Streptococcus pyogenes (group A streptococci, GAS) two-component signal transduction system CovRS has been described to be important for pathogenesis of this exclusively human bacterial species. If this system acts uniquely in all serotypes is currently unclear. Presence of serotype-...

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Autores principales: Sugareva, Venelina, Arlt, Regina, Fiedler, Tomas, Riani, Catur, Podbielski, Andreas, Kreikemeyer, Bernd
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823723/
https://www.ncbi.nlm.nih.gov/pubmed/20113532
http://dx.doi.org/10.1186/1471-2180-10-34
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author Sugareva, Venelina
Arlt, Regina
Fiedler, Tomas
Riani, Catur
Podbielski, Andreas
Kreikemeyer, Bernd
author_facet Sugareva, Venelina
Arlt, Regina
Fiedler, Tomas
Riani, Catur
Podbielski, Andreas
Kreikemeyer, Bernd
author_sort Sugareva, Venelina
collection PubMed
description BACKGROUND: The Streptococcus pyogenes (group A streptococci, GAS) two-component signal transduction system CovRS has been described to be important for pathogenesis of this exclusively human bacterial species. If this system acts uniquely in all serotypes is currently unclear. Presence of serotype- or strain-dependent regulatory circuits and polarity is an emerging scheme in Streptococcus pyogenes pathogenesis. Thus, the contribution of the sensor kinase (CovS) of the global regulatory two-component signal transduction system CovRS on pathogenesis of several M serotypes was investigated. RESULTS: CovS mutation uniformly repressed capsule expression and hampered keratinocyte adherence in all tested serotypes. However, a serotype- and even strain-dependent contribution on survival in whole human blood and biofilm formation was noted, respectively. CONCLUSIONS: These data provide new information on the action of the CovS sensor kinase and revealed that its activity on capsule expression and keratinocyte adherence is uniform across serotypes, whereas the influence on biofilm formation and blood survival is serotype or even strain dependent. This adds the CovRS system to a growing list of serotype-specific acting regulatory loci in S. pyogenes.
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spelling pubmed-28237232010-02-18 Serotype- and strain- dependent contribution of the sensor kinase CovS of the CovRS two-component system to Streptococcus pyogenes pathogenesis Sugareva, Venelina Arlt, Regina Fiedler, Tomas Riani, Catur Podbielski, Andreas Kreikemeyer, Bernd BMC Microbiol Research article BACKGROUND: The Streptococcus pyogenes (group A streptococci, GAS) two-component signal transduction system CovRS has been described to be important for pathogenesis of this exclusively human bacterial species. If this system acts uniquely in all serotypes is currently unclear. Presence of serotype- or strain-dependent regulatory circuits and polarity is an emerging scheme in Streptococcus pyogenes pathogenesis. Thus, the contribution of the sensor kinase (CovS) of the global regulatory two-component signal transduction system CovRS on pathogenesis of several M serotypes was investigated. RESULTS: CovS mutation uniformly repressed capsule expression and hampered keratinocyte adherence in all tested serotypes. However, a serotype- and even strain-dependent contribution on survival in whole human blood and biofilm formation was noted, respectively. CONCLUSIONS: These data provide new information on the action of the CovS sensor kinase and revealed that its activity on capsule expression and keratinocyte adherence is uniform across serotypes, whereas the influence on biofilm formation and blood survival is serotype or even strain dependent. This adds the CovRS system to a growing list of serotype-specific acting regulatory loci in S. pyogenes. BioMed Central 2010-02-01 /pmc/articles/PMC2823723/ /pubmed/20113532 http://dx.doi.org/10.1186/1471-2180-10-34 Text en Copyright ©2010 Sugareva et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Sugareva, Venelina
Arlt, Regina
Fiedler, Tomas
Riani, Catur
Podbielski, Andreas
Kreikemeyer, Bernd
Serotype- and strain- dependent contribution of the sensor kinase CovS of the CovRS two-component system to Streptococcus pyogenes pathogenesis
title Serotype- and strain- dependent contribution of the sensor kinase CovS of the CovRS two-component system to Streptococcus pyogenes pathogenesis
title_full Serotype- and strain- dependent contribution of the sensor kinase CovS of the CovRS two-component system to Streptococcus pyogenes pathogenesis
title_fullStr Serotype- and strain- dependent contribution of the sensor kinase CovS of the CovRS two-component system to Streptococcus pyogenes pathogenesis
title_full_unstemmed Serotype- and strain- dependent contribution of the sensor kinase CovS of the CovRS two-component system to Streptococcus pyogenes pathogenesis
title_short Serotype- and strain- dependent contribution of the sensor kinase CovS of the CovRS two-component system to Streptococcus pyogenes pathogenesis
title_sort serotype- and strain- dependent contribution of the sensor kinase covs of the covrs two-component system to streptococcus pyogenes pathogenesis
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2823723/
https://www.ncbi.nlm.nih.gov/pubmed/20113532
http://dx.doi.org/10.1186/1471-2180-10-34
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