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Developmental cues and persistent neurogenic potential within an in vitro neural niche
BACKGROUND: Neurogenesis, the production of neural cell-types from neural stem cells (NSCs), occurs during development as well as within select regions of the adult brain. NSCs in the adult subependymal zone (SEZ) exist in a well-categorized niche microenvironment established by surrounding cells an...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2824744/ https://www.ncbi.nlm.nih.gov/pubmed/20074373 http://dx.doi.org/10.1186/1471-213X-10-5 |
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author | Pierret, Chris Morrison, Jason A Rath, Prakash Zigler, Rachel E Engel, Laura A Fairchild, Corinne L Shi, Huidong Maruniak, Joel A Kirk, Mark D |
author_facet | Pierret, Chris Morrison, Jason A Rath, Prakash Zigler, Rachel E Engel, Laura A Fairchild, Corinne L Shi, Huidong Maruniak, Joel A Kirk, Mark D |
author_sort | Pierret, Chris |
collection | PubMed |
description | BACKGROUND: Neurogenesis, the production of neural cell-types from neural stem cells (NSCs), occurs during development as well as within select regions of the adult brain. NSCs in the adult subependymal zone (SEZ) exist in a well-categorized niche microenvironment established by surrounding cells and their molecular products. The components of this niche maintain the NSCs and their definitive properties, including the ability to self-renew and multipotency (neuronal and glial differentiation). RESULTS: We describe a model in vitro NSC niche, derived from embryonic stem cells, that produces many of the cells and products of the developing subventricular zone (SVZ) and adult SEZ NSC niche. We demonstrate a possible role for apoptosis and for components of the extracellular matrix in the maintenance of the NSC population within our niche cultures. We characterize expression of genes relevant to NSC self-renewal and the process of neurogenesis and compare these findings to gene expression produced by an established neural-induction protocol employing retinoic acid. CONCLUSIONS: The in vitro NSC niche shows an identity that is distinct from the neurally induced embryonic cells that were used to derive it. Molecular and cellular components found in our in vitro NSC niche include NSCs, neural progeny, and ECM components and their receptors. Establishment of the in vitro NSC niche occurs in conjunction with apoptosis. Applications of this culture system range from studies of signaling events fundamental to niche formation and maintenance as well as development of unique NSC transplant platforms to treat disease or injury. |
format | Text |
id | pubmed-2824744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28247442010-02-20 Developmental cues and persistent neurogenic potential within an in vitro neural niche Pierret, Chris Morrison, Jason A Rath, Prakash Zigler, Rachel E Engel, Laura A Fairchild, Corinne L Shi, Huidong Maruniak, Joel A Kirk, Mark D BMC Dev Biol Research article BACKGROUND: Neurogenesis, the production of neural cell-types from neural stem cells (NSCs), occurs during development as well as within select regions of the adult brain. NSCs in the adult subependymal zone (SEZ) exist in a well-categorized niche microenvironment established by surrounding cells and their molecular products. The components of this niche maintain the NSCs and their definitive properties, including the ability to self-renew and multipotency (neuronal and glial differentiation). RESULTS: We describe a model in vitro NSC niche, derived from embryonic stem cells, that produces many of the cells and products of the developing subventricular zone (SVZ) and adult SEZ NSC niche. We demonstrate a possible role for apoptosis and for components of the extracellular matrix in the maintenance of the NSC population within our niche cultures. We characterize expression of genes relevant to NSC self-renewal and the process of neurogenesis and compare these findings to gene expression produced by an established neural-induction protocol employing retinoic acid. CONCLUSIONS: The in vitro NSC niche shows an identity that is distinct from the neurally induced embryonic cells that were used to derive it. Molecular and cellular components found in our in vitro NSC niche include NSCs, neural progeny, and ECM components and their receptors. Establishment of the in vitro NSC niche occurs in conjunction with apoptosis. Applications of this culture system range from studies of signaling events fundamental to niche formation and maintenance as well as development of unique NSC transplant platforms to treat disease or injury. BioMed Central 2010-01-14 /pmc/articles/PMC2824744/ /pubmed/20074373 http://dx.doi.org/10.1186/1471-213X-10-5 Text en Copyright ©2010 Pierret et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research article Pierret, Chris Morrison, Jason A Rath, Prakash Zigler, Rachel E Engel, Laura A Fairchild, Corinne L Shi, Huidong Maruniak, Joel A Kirk, Mark D Developmental cues and persistent neurogenic potential within an in vitro neural niche |
title | Developmental cues and persistent neurogenic potential within an in vitro neural niche |
title_full | Developmental cues and persistent neurogenic potential within an in vitro neural niche |
title_fullStr | Developmental cues and persistent neurogenic potential within an in vitro neural niche |
title_full_unstemmed | Developmental cues and persistent neurogenic potential within an in vitro neural niche |
title_short | Developmental cues and persistent neurogenic potential within an in vitro neural niche |
title_sort | developmental cues and persistent neurogenic potential within an in vitro neural niche |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2824744/ https://www.ncbi.nlm.nih.gov/pubmed/20074373 http://dx.doi.org/10.1186/1471-213X-10-5 |
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