Cargando…
Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes
Potato virus M (PVM, Carlavirus) is considered to be one of the most common potato viruses distributed worldwide. Sequences of the coat protein (CP) gene of several Canadian PVM isolates were determined. Phylogenetic analysis indicated that all known PVM isolates fell into two distinct groups and th...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2825510/ https://www.ncbi.nlm.nih.gov/pubmed/20122168 http://dx.doi.org/10.1186/1743-422X-7-25 |
_version_ | 1782177817576865792 |
---|---|
author | Xu, Huimin D'Aubin, Jeanette Nie, Jingbai |
author_facet | Xu, Huimin D'Aubin, Jeanette Nie, Jingbai |
author_sort | Xu, Huimin |
collection | PubMed |
description | Potato virus M (PVM, Carlavirus) is considered to be one of the most common potato viruses distributed worldwide. Sequences of the coat protein (CP) gene of several Canadian PVM isolates were determined. Phylogenetic analysis indicated that all known PVM isolates fell into two distinct groups and the isolates from Canada and the US clustered in the same group. The Canadian PVM isolates could be further divided into two sub-groups. Two molecular procedures, reverse transcription - polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP) were developed in this study for the detection and identification of PVM in potato tubers. RT-PCR was highly specific and only amplified PVM RNA from potato samples. PVM RNAs were easily detected in composite samples of 400 to 800 potato leaves or 200 to 400 dormant tubers. Restriction analysis of PCR amplicons with MscI was a simple method for the confirmation of PCR tests. Thus, RT-PCR followed by RFLP analysis may be a useful approach for screening potato samples on a large scale for the presence of PVM. |
format | Text |
id | pubmed-2825510 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28255102010-02-21 Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes Xu, Huimin D'Aubin, Jeanette Nie, Jingbai Virol J Research Potato virus M (PVM, Carlavirus) is considered to be one of the most common potato viruses distributed worldwide. Sequences of the coat protein (CP) gene of several Canadian PVM isolates were determined. Phylogenetic analysis indicated that all known PVM isolates fell into two distinct groups and the isolates from Canada and the US clustered in the same group. The Canadian PVM isolates could be further divided into two sub-groups. Two molecular procedures, reverse transcription - polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP) were developed in this study for the detection and identification of PVM in potato tubers. RT-PCR was highly specific and only amplified PVM RNA from potato samples. PVM RNAs were easily detected in composite samples of 400 to 800 potato leaves or 200 to 400 dormant tubers. Restriction analysis of PCR amplicons with MscI was a simple method for the confirmation of PCR tests. Thus, RT-PCR followed by RFLP analysis may be a useful approach for screening potato samples on a large scale for the presence of PVM. BioMed Central 2010-02-01 /pmc/articles/PMC2825510/ /pubmed/20122168 http://dx.doi.org/10.1186/1743-422X-7-25 Text en Copyright ©2010 Xu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Xu, Huimin D'Aubin, Jeanette Nie, Jingbai Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes |
title | Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes |
title_full | Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes |
title_fullStr | Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes |
title_full_unstemmed | Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes |
title_short | Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes |
title_sort | genomic variability in potato virus m and the development of rt-pcr and rflp procedures for the detection of this virus in seed potatoes |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2825510/ https://www.ncbi.nlm.nih.gov/pubmed/20122168 http://dx.doi.org/10.1186/1743-422X-7-25 |
work_keys_str_mv | AT xuhuimin genomicvariabilityinpotatovirusmandthedevelopmentofrtpcrandrflpproceduresforthedetectionofthisvirusinseedpotatoes AT daubinjeanette genomicvariabilityinpotatovirusmandthedevelopmentofrtpcrandrflpproceduresforthedetectionofthisvirusinseedpotatoes AT niejingbai genomicvariabilityinpotatovirusmandthedevelopmentofrtpcrandrflpproceduresforthedetectionofthisvirusinseedpotatoes |