Influence of Genetic Background and Tissue Types on Global DNA Methylation Patterns

Recent studies have shown a genetic influence on gene expression variation, chromatin, and DNA methylation. However, the effects of genetic background and tissue types on DNA methylation at the genome-wide level have not been characterized extensively. To study the effect of genetic background and t...

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Autores principales: Yang, Howard H., Hu, Nan, Wang, Chaoyu, Ding, Ti, Dunn, Barbara K., Goldstein, Alisa M., Taylor, Philip R., Lee, Maxwell P.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826396/
https://www.ncbi.nlm.nih.gov/pubmed/20186319
http://dx.doi.org/10.1371/journal.pone.0009355
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author Yang, Howard H.
Hu, Nan
Wang, Chaoyu
Ding, Ti
Dunn, Barbara K.
Goldstein, Alisa M.
Taylor, Philip R.
Lee, Maxwell P.
author_facet Yang, Howard H.
Hu, Nan
Wang, Chaoyu
Ding, Ti
Dunn, Barbara K.
Goldstein, Alisa M.
Taylor, Philip R.
Lee, Maxwell P.
author_sort Yang, Howard H.
collection PubMed
description Recent studies have shown a genetic influence on gene expression variation, chromatin, and DNA methylation. However, the effects of genetic background and tissue types on DNA methylation at the genome-wide level have not been characterized extensively. To study the effect of genetic background and tissue types on global DNA methylation, we performed DNA methylation analysis using the Affymetrix 500K SNP array on tumor, adjacent normal tissue, and blood DNA from 30 patients with esophageal squamous cell carcinoma (ESCC). The use of multiple tissues from 30 individuals allowed us to evaluate variation of DNA methylation states across tissues and individuals. Our results demonstrate that blood and esophageal tissues shared similar DNA methylation patterns within the same individual, suggesting an influence of genetic background on DNA methylation. Furthermore, we showed that tissue types are important contributors of DNA methylation states.
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spelling pubmed-28263962010-02-26 Influence of Genetic Background and Tissue Types on Global DNA Methylation Patterns Yang, Howard H. Hu, Nan Wang, Chaoyu Ding, Ti Dunn, Barbara K. Goldstein, Alisa M. Taylor, Philip R. Lee, Maxwell P. PLoS One Research Article Recent studies have shown a genetic influence on gene expression variation, chromatin, and DNA methylation. However, the effects of genetic background and tissue types on DNA methylation at the genome-wide level have not been characterized extensively. To study the effect of genetic background and tissue types on global DNA methylation, we performed DNA methylation analysis using the Affymetrix 500K SNP array on tumor, adjacent normal tissue, and blood DNA from 30 patients with esophageal squamous cell carcinoma (ESCC). The use of multiple tissues from 30 individuals allowed us to evaluate variation of DNA methylation states across tissues and individuals. Our results demonstrate that blood and esophageal tissues shared similar DNA methylation patterns within the same individual, suggesting an influence of genetic background on DNA methylation. Furthermore, we showed that tissue types are important contributors of DNA methylation states. Public Library of Science 2010-02-23 /pmc/articles/PMC2826396/ /pubmed/20186319 http://dx.doi.org/10.1371/journal.pone.0009355 Text en This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Yang, Howard H.
Hu, Nan
Wang, Chaoyu
Ding, Ti
Dunn, Barbara K.
Goldstein, Alisa M.
Taylor, Philip R.
Lee, Maxwell P.
Influence of Genetic Background and Tissue Types on Global DNA Methylation Patterns
title Influence of Genetic Background and Tissue Types on Global DNA Methylation Patterns
title_full Influence of Genetic Background and Tissue Types on Global DNA Methylation Patterns
title_fullStr Influence of Genetic Background and Tissue Types on Global DNA Methylation Patterns
title_full_unstemmed Influence of Genetic Background and Tissue Types on Global DNA Methylation Patterns
title_short Influence of Genetic Background and Tissue Types on Global DNA Methylation Patterns
title_sort influence of genetic background and tissue types on global dna methylation patterns
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826396/
https://www.ncbi.nlm.nih.gov/pubmed/20186319
http://dx.doi.org/10.1371/journal.pone.0009355
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