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Redox states of glutathione and ascorbate in root tips of poplar (Populus tremula×P. alba) depend on phloem transport from the shoot to the roots
Glutathione (GSH) and ascorbate (ASC) are important antioxidants that are involved in stress defence and cell proliferation of meristematic root cells. In principle, synthesis of ASC and GSH in the roots as well as ASC and GSH transport from the shoot to the roots by phloem mass flow is possible. Ho...
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826650/ https://www.ncbi.nlm.nih.gov/pubmed/20022923 http://dx.doi.org/10.1093/jxb/erp371 |
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author | Herschbach, Cornelia Scheerer, Ursula Rennenberg, Heinz |
author_facet | Herschbach, Cornelia Scheerer, Ursula Rennenberg, Heinz |
author_sort | Herschbach, Cornelia |
collection | PubMed |
description | Glutathione (GSH) and ascorbate (ASC) are important antioxidants that are involved in stress defence and cell proliferation of meristematic root cells. In principle, synthesis of ASC and GSH in the roots as well as ASC and GSH transport from the shoot to the roots by phloem mass flow is possible. However, it is not yet known whether the ASC and/or the GSH level in roots depends on the supply from the shoot. This was analysed by feeding mature leaves with [(14)C]ASC or [(35)S]GSH and subsequent detection of the radiolabel in different root fractions. Quantitative dependency of root ASC and GSH on shoot-derived ASC and GSH was investigated with poplar (Populus tremula×P. alba) trees interrupted in phloem transport. [(35)S]GSH is transported from mature leaves to the root tips, but is withdrawn from the phloem along the entire transport path. When phloem transport was interrupted, the GSH content in root tips halved within 3 d. [(14)C]ASC is also transported from mature leaves to the root tips but, in contrast to GSH, ASC is not removed from the phloem along the transport path. Accordingly, ASC accumulates in root tips. Interruption of phloem transport disturbed the level and the ASC redox state within the entire root system. Diminished total ASC levels were attributed mainly to a decline of dehydroascorbate (DHA). As the redox state of ASC is of particular significance for root growth and development, it is concluded that phloem transport of ASC may constitute a shoot to root signal to coordinate growth and development at the whole plant level. |
format | Text |
id | pubmed-2826650 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-28266502010-02-24 Redox states of glutathione and ascorbate in root tips of poplar (Populus tremula×P. alba) depend on phloem transport from the shoot to the roots Herschbach, Cornelia Scheerer, Ursula Rennenberg, Heinz J Exp Bot Research Papers Glutathione (GSH) and ascorbate (ASC) are important antioxidants that are involved in stress defence and cell proliferation of meristematic root cells. In principle, synthesis of ASC and GSH in the roots as well as ASC and GSH transport from the shoot to the roots by phloem mass flow is possible. However, it is not yet known whether the ASC and/or the GSH level in roots depends on the supply from the shoot. This was analysed by feeding mature leaves with [(14)C]ASC or [(35)S]GSH and subsequent detection of the radiolabel in different root fractions. Quantitative dependency of root ASC and GSH on shoot-derived ASC and GSH was investigated with poplar (Populus tremula×P. alba) trees interrupted in phloem transport. [(35)S]GSH is transported from mature leaves to the root tips, but is withdrawn from the phloem along the entire transport path. When phloem transport was interrupted, the GSH content in root tips halved within 3 d. [(14)C]ASC is also transported from mature leaves to the root tips but, in contrast to GSH, ASC is not removed from the phloem along the transport path. Accordingly, ASC accumulates in root tips. Interruption of phloem transport disturbed the level and the ASC redox state within the entire root system. Diminished total ASC levels were attributed mainly to a decline of dehydroascorbate (DHA). As the redox state of ASC is of particular significance for root growth and development, it is concluded that phloem transport of ASC may constitute a shoot to root signal to coordinate growth and development at the whole plant level. Oxford University Press 2010-02 2009-12-18 /pmc/articles/PMC2826650/ /pubmed/20022923 http://dx.doi.org/10.1093/jxb/erp371 Text en © 2009 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details) |
spellingShingle | Research Papers Herschbach, Cornelia Scheerer, Ursula Rennenberg, Heinz Redox states of glutathione and ascorbate in root tips of poplar (Populus tremula×P. alba) depend on phloem transport from the shoot to the roots |
title | Redox states of glutathione and ascorbate in root tips of poplar (Populus tremula×P. alba) depend on phloem transport from the shoot to the roots |
title_full | Redox states of glutathione and ascorbate in root tips of poplar (Populus tremula×P. alba) depend on phloem transport from the shoot to the roots |
title_fullStr | Redox states of glutathione and ascorbate in root tips of poplar (Populus tremula×P. alba) depend on phloem transport from the shoot to the roots |
title_full_unstemmed | Redox states of glutathione and ascorbate in root tips of poplar (Populus tremula×P. alba) depend on phloem transport from the shoot to the roots |
title_short | Redox states of glutathione and ascorbate in root tips of poplar (Populus tremula×P. alba) depend on phloem transport from the shoot to the roots |
title_sort | redox states of glutathione and ascorbate in root tips of poplar (populus tremula×p. alba) depend on phloem transport from the shoot to the roots |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826650/ https://www.ncbi.nlm.nih.gov/pubmed/20022923 http://dx.doi.org/10.1093/jxb/erp371 |
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