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Plasma proteome analysis of patients with type 1 diabetes with diabetic nephropathy
BACKGROUND: As part of a clinical proteomics program focused on diabetes and its complications we are looking for new and better protein biomarkers for diabetic nephropathy. The search for new and better biomarkers for diabetic nephropathy has, with a few exceptions, previously focused on either hyp...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2827395/ https://www.ncbi.nlm.nih.gov/pubmed/20205888 http://dx.doi.org/10.1186/1477-5956-8-4 |
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author | Overgaard, Anne Julie Hansen, Henning Gram Lajer, Maria Pedersen, Lykke Tarnow, Lise Rossing, Peter McGuire, James N Pociot, Flemming |
author_facet | Overgaard, Anne Julie Hansen, Henning Gram Lajer, Maria Pedersen, Lykke Tarnow, Lise Rossing, Peter McGuire, James N Pociot, Flemming |
author_sort | Overgaard, Anne Julie |
collection | PubMed |
description | BACKGROUND: As part of a clinical proteomics program focused on diabetes and its complications we are looking for new and better protein biomarkers for diabetic nephropathy. The search for new and better biomarkers for diabetic nephropathy has, with a few exceptions, previously focused on either hypothesis-driven studies or urinary based investigations. To date only two studies have investigated the proteome of blood in search for new biomarkers, and these studies were conducted in sera from patients with type 2 diabetes. This is the first reported in depth proteomic study where plasma from type 1 diabetic patients was investigated with the goal of finding improved candidate biomarkers to predict diabetic nephropathy. In order to reach lower concentration proteins in plasma a pre-fractionation step, either hexapeptide bead-based libraries or anion exchange chromatography, was performed prior to surface enhanced laser desorption/ionization time-of-flight mass spectrometry analysis. RESULTS: Proteomic analysis of plasma from a cross-sectional cohort of 123 type 1 diabetic patients previously diagnosed as normoalbuminuric, microalbuminuric or macroalbuminuric, gave rise to 290 peaks clusters of which 16 were selected as the most promising biomarker candidates based on statistical performance, including independent component analysis. Four of the peaks that were discovered have been identified as transthyretin, apolipoprotein A1, apolipoprotein C1 and cystatin C. Several yet unidentified proteins discovered by this novel approach appear to have more potential as biomarkers for diabetic nephropathy. CONCLUSION: These results demonstrate the capacity of proteomic analysis of plasma, by confirming the presence of known biomarkers as well as revealing new biomarkers for diabetic nephropathy in plasma in type 1 diabetic patients. |
format | Text |
id | pubmed-2827395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28273952010-02-24 Plasma proteome analysis of patients with type 1 diabetes with diabetic nephropathy Overgaard, Anne Julie Hansen, Henning Gram Lajer, Maria Pedersen, Lykke Tarnow, Lise Rossing, Peter McGuire, James N Pociot, Flemming Proteome Sci Research BACKGROUND: As part of a clinical proteomics program focused on diabetes and its complications we are looking for new and better protein biomarkers for diabetic nephropathy. The search for new and better biomarkers for diabetic nephropathy has, with a few exceptions, previously focused on either hypothesis-driven studies or urinary based investigations. To date only two studies have investigated the proteome of blood in search for new biomarkers, and these studies were conducted in sera from patients with type 2 diabetes. This is the first reported in depth proteomic study where plasma from type 1 diabetic patients was investigated with the goal of finding improved candidate biomarkers to predict diabetic nephropathy. In order to reach lower concentration proteins in plasma a pre-fractionation step, either hexapeptide bead-based libraries or anion exchange chromatography, was performed prior to surface enhanced laser desorption/ionization time-of-flight mass spectrometry analysis. RESULTS: Proteomic analysis of plasma from a cross-sectional cohort of 123 type 1 diabetic patients previously diagnosed as normoalbuminuric, microalbuminuric or macroalbuminuric, gave rise to 290 peaks clusters of which 16 were selected as the most promising biomarker candidates based on statistical performance, including independent component analysis. Four of the peaks that were discovered have been identified as transthyretin, apolipoprotein A1, apolipoprotein C1 and cystatin C. Several yet unidentified proteins discovered by this novel approach appear to have more potential as biomarkers for diabetic nephropathy. CONCLUSION: These results demonstrate the capacity of proteomic analysis of plasma, by confirming the presence of known biomarkers as well as revealing new biomarkers for diabetic nephropathy in plasma in type 1 diabetic patients. BioMed Central 2010-02-03 /pmc/articles/PMC2827395/ /pubmed/20205888 http://dx.doi.org/10.1186/1477-5956-8-4 Text en Copyright ©2010 Overgaard et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Overgaard, Anne Julie Hansen, Henning Gram Lajer, Maria Pedersen, Lykke Tarnow, Lise Rossing, Peter McGuire, James N Pociot, Flemming Plasma proteome analysis of patients with type 1 diabetes with diabetic nephropathy |
title | Plasma proteome analysis of patients with type 1 diabetes with diabetic nephropathy |
title_full | Plasma proteome analysis of patients with type 1 diabetes with diabetic nephropathy |
title_fullStr | Plasma proteome analysis of patients with type 1 diabetes with diabetic nephropathy |
title_full_unstemmed | Plasma proteome analysis of patients with type 1 diabetes with diabetic nephropathy |
title_short | Plasma proteome analysis of patients with type 1 diabetes with diabetic nephropathy |
title_sort | plasma proteome analysis of patients with type 1 diabetes with diabetic nephropathy |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2827395/ https://www.ncbi.nlm.nih.gov/pubmed/20205888 http://dx.doi.org/10.1186/1477-5956-8-4 |
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