PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli

We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%–20% of hybridizable plasmid genes: hl...

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Detalles Bibliográficos
Autores principales: Gerrish, Robert S., Lee, James E., Reed, June, Williams, Joel, Farrell, Larry D., Spiegel, Kathleen M., Sheridan, Peter P., Shields, Malcolm S.
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2007
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2828064/
https://www.ncbi.nlm.nih.gov/pubmed/17953106
http://dx.doi.org/10.3201/eid1308.060428
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author Gerrish, Robert S.
Lee, James E.
Reed, June
Williams, Joel
Farrell, Larry D.
Spiegel, Kathleen M.
Sheridan, Peter P.
Shields, Malcolm S.
author_facet Gerrish, Robert S.
Lee, James E.
Reed, June
Williams, Joel
Farrell, Larry D.
Spiegel, Kathleen M.
Sheridan, Peter P.
Shields, Malcolm S.
author_sort Gerrish, Robert S.
collection PubMed
description We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%–20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2.
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spelling pubmed-28280642010-03-02 PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli Gerrish, Robert S. Lee, James E. Reed, June Williams, Joel Farrell, Larry D. Spiegel, Kathleen M. Sheridan, Peter P. Shields, Malcolm S. Emerg Infect Dis Dispatch We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%–20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2. Centers for Disease Control and Prevention 2007-08 /pmc/articles/PMC2828064/ /pubmed/17953106 http://dx.doi.org/10.3201/eid1308.060428 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Gerrish, Robert S.
Lee, James E.
Reed, June
Williams, Joel
Farrell, Larry D.
Spiegel, Kathleen M.
Sheridan, Peter P.
Shields, Malcolm S.
PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli
title PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli
title_full PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli
title_fullStr PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli
title_full_unstemmed PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli
title_short PCR versus Hybridization for Detecting Virulence Genes of Enterohemorrhagic Escherichia coli
title_sort pcr versus hybridization for detecting virulence genes of enterohemorrhagic escherichia coli
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2828064/
https://www.ncbi.nlm.nih.gov/pubmed/17953106
http://dx.doi.org/10.3201/eid1308.060428
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