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The Genome of Streptococcus mitis B6 - What Is a Commensal?
Streptococcus mitis is the closest relative of the major human pathogen S. pneumoniae. The 2,15 Mb sequence of the Streptococcus mitis B6 chromosome, an unusually high-level beta-lactam resistant and multiple antibiotic resistant strain, has now been determined to encode 2100 genes. The accessory ge...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2828477/ https://www.ncbi.nlm.nih.gov/pubmed/20195536 http://dx.doi.org/10.1371/journal.pone.0009426 |
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author | Denapaite, Dalia Brückner, Reinhold Nuhn, Michael Reichmann, Peter Henrich, Bernhard Maurer, Patrick Schähle, Yvonne Selbmann, Peter Zimmermann, Wolfgang Wambutt, Rolf Hakenbeck, Regine |
author_facet | Denapaite, Dalia Brückner, Reinhold Nuhn, Michael Reichmann, Peter Henrich, Bernhard Maurer, Patrick Schähle, Yvonne Selbmann, Peter Zimmermann, Wolfgang Wambutt, Rolf Hakenbeck, Regine |
author_sort | Denapaite, Dalia |
collection | PubMed |
description | Streptococcus mitis is the closest relative of the major human pathogen S. pneumoniae. The 2,15 Mb sequence of the Streptococcus mitis B6 chromosome, an unusually high-level beta-lactam resistant and multiple antibiotic resistant strain, has now been determined to encode 2100 genes. The accessory genome is estimated to represent over 40%, including 75 mostly novel transposases and IS, the prophage φB6 and another seven phage related regions. Tetracycline resistance mediated by Tn5801, and an unusual and large gene cluster containing three aminoglycoside resistance determinants have not been described in other Streptococcus spp. Comparative genomic analyses including hybridization experiments on a S. mitis B6 specific microarray reveal that individual S. mitis strains are almost as distantly related to the B6 strain as S. pneumoniae. Both species share a core of over 900 genes. Most proteins described as pneumococcal virulence factors are present in S. mitis B6, but the three choline binding proteins PcpA, PspA and PspC, and three gene clusters containing the hyaluronidase gene, ply and lytA, and the capsular genes are absent in S. mitis B6 and other S. mitis as well and confirm their importance for the pathogenetic potential of S. pneumoniae. Despite the close relatedness between the two species, the S. mitis B6 genome reveals a striking X-alignment when compared with S. pneumoniae. |
format | Text |
id | pubmed-2828477 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28284772010-03-02 The Genome of Streptococcus mitis B6 - What Is a Commensal? Denapaite, Dalia Brückner, Reinhold Nuhn, Michael Reichmann, Peter Henrich, Bernhard Maurer, Patrick Schähle, Yvonne Selbmann, Peter Zimmermann, Wolfgang Wambutt, Rolf Hakenbeck, Regine PLoS One Research Article Streptococcus mitis is the closest relative of the major human pathogen S. pneumoniae. The 2,15 Mb sequence of the Streptococcus mitis B6 chromosome, an unusually high-level beta-lactam resistant and multiple antibiotic resistant strain, has now been determined to encode 2100 genes. The accessory genome is estimated to represent over 40%, including 75 mostly novel transposases and IS, the prophage φB6 and another seven phage related regions. Tetracycline resistance mediated by Tn5801, and an unusual and large gene cluster containing three aminoglycoside resistance determinants have not been described in other Streptococcus spp. Comparative genomic analyses including hybridization experiments on a S. mitis B6 specific microarray reveal that individual S. mitis strains are almost as distantly related to the B6 strain as S. pneumoniae. Both species share a core of over 900 genes. Most proteins described as pneumococcal virulence factors are present in S. mitis B6, but the three choline binding proteins PcpA, PspA and PspC, and three gene clusters containing the hyaluronidase gene, ply and lytA, and the capsular genes are absent in S. mitis B6 and other S. mitis as well and confirm their importance for the pathogenetic potential of S. pneumoniae. Despite the close relatedness between the two species, the S. mitis B6 genome reveals a striking X-alignment when compared with S. pneumoniae. Public Library of Science 2010-02-25 /pmc/articles/PMC2828477/ /pubmed/20195536 http://dx.doi.org/10.1371/journal.pone.0009426 Text en Denapaite et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Denapaite, Dalia Brückner, Reinhold Nuhn, Michael Reichmann, Peter Henrich, Bernhard Maurer, Patrick Schähle, Yvonne Selbmann, Peter Zimmermann, Wolfgang Wambutt, Rolf Hakenbeck, Regine The Genome of Streptococcus mitis B6 - What Is a Commensal? |
title | The Genome of Streptococcus mitis B6 - What Is a Commensal? |
title_full | The Genome of Streptococcus mitis B6 - What Is a Commensal? |
title_fullStr | The Genome of Streptococcus mitis B6 - What Is a Commensal? |
title_full_unstemmed | The Genome of Streptococcus mitis B6 - What Is a Commensal? |
title_short | The Genome of Streptococcus mitis B6 - What Is a Commensal? |
title_sort | genome of streptococcus mitis b6 - what is a commensal? |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2828477/ https://www.ncbi.nlm.nih.gov/pubmed/20195536 http://dx.doi.org/10.1371/journal.pone.0009426 |
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