Cargando…
A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia
A tospovirus causing necrotic streaks on leaves was isolated from Alstroemeria sp. in Colombia. Infected samples reacted positively with tomato spotted wilt virus (TSWV) antiserum during preliminary serological tests. Further analysis revealed a close serological relationship to tomato chlorotic spo...
Autores principales: | , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer Vienna
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2828552/ https://www.ncbi.nlm.nih.gov/pubmed/20151164 http://dx.doi.org/10.1007/s00705-010-0590-7 |
_version_ | 1782178023677624320 |
---|---|
author | Hassani-Mehraban, Afshin Botermans, Marleen Verhoeven, J. Th. J. Meekes, Ellis Saaijer, Janneke Peters, Dick Goldbach, Rob Kormelink, Richard |
author_facet | Hassani-Mehraban, Afshin Botermans, Marleen Verhoeven, J. Th. J. Meekes, Ellis Saaijer, Janneke Peters, Dick Goldbach, Rob Kormelink, Richard |
author_sort | Hassani-Mehraban, Afshin |
collection | PubMed |
description | A tospovirus causing necrotic streaks on leaves was isolated from Alstroemeria sp. in Colombia. Infected samples reacted positively with tomato spotted wilt virus (TSWV) antiserum during preliminary serological tests. Further analysis revealed a close serological relationship to tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV). A major part of the S-RNA segment, encompassing the nucleocapsid (N) protein gene, the 5′ untranslated region and a part of the intergenic region 3′ of the N gene, was cloned and sequenced. The deduced N protein sequence showed highest amino acid identity (82%) to that of TCSV, indicating that the virus represents a new tospovirus species, for which the name Alstroemeria necrotic streak virus (ANSV) is coined. Phylogenetic analysis based on the N protein sequence revealed that this Alstroemeria-infecting tospovirus clustered with tospoviruses from the American continent. Frankliniella occidentalis was identified as potential vector species for ANSV. |
format | Text |
id | pubmed-2828552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Springer Vienna |
record_format | MEDLINE/PubMed |
spelling | pubmed-28285522010-03-05 A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia Hassani-Mehraban, Afshin Botermans, Marleen Verhoeven, J. Th. J. Meekes, Ellis Saaijer, Janneke Peters, Dick Goldbach, Rob Kormelink, Richard Arch Virol Brief Report A tospovirus causing necrotic streaks on leaves was isolated from Alstroemeria sp. in Colombia. Infected samples reacted positively with tomato spotted wilt virus (TSWV) antiserum during preliminary serological tests. Further analysis revealed a close serological relationship to tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV). A major part of the S-RNA segment, encompassing the nucleocapsid (N) protein gene, the 5′ untranslated region and a part of the intergenic region 3′ of the N gene, was cloned and sequenced. The deduced N protein sequence showed highest amino acid identity (82%) to that of TCSV, indicating that the virus represents a new tospovirus species, for which the name Alstroemeria necrotic streak virus (ANSV) is coined. Phylogenetic analysis based on the N protein sequence revealed that this Alstroemeria-infecting tospovirus clustered with tospoviruses from the American continent. Frankliniella occidentalis was identified as potential vector species for ANSV. Springer Vienna 2010-02-12 2010 /pmc/articles/PMC2828552/ /pubmed/20151164 http://dx.doi.org/10.1007/s00705-010-0590-7 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Brief Report Hassani-Mehraban, Afshin Botermans, Marleen Verhoeven, J. Th. J. Meekes, Ellis Saaijer, Janneke Peters, Dick Goldbach, Rob Kormelink, Richard A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia |
title | A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia |
title_full | A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia |
title_fullStr | A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia |
title_full_unstemmed | A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia |
title_short | A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia |
title_sort | distinct tospovirus causing necrotic streak on alstroemeria sp. in colombia |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2828552/ https://www.ncbi.nlm.nih.gov/pubmed/20151164 http://dx.doi.org/10.1007/s00705-010-0590-7 |
work_keys_str_mv | AT hassanimehrabanafshin adistincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT botermansmarleen adistincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT verhoevenjthj adistincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT meekesellis adistincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT saaijerjanneke adistincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT petersdick adistincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT goldbachrob adistincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT kormelinkrichard adistincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT hassanimehrabanafshin distincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT botermansmarleen distincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT verhoevenjthj distincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT meekesellis distincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT saaijerjanneke distincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT petersdick distincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT goldbachrob distincttospoviruscausingnecroticstreakonalstroemeriaspincolombia AT kormelinkrichard distincttospoviruscausingnecroticstreakonalstroemeriaspincolombia |