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Local Membrane Deformations Activate Ca(2+)-Dependent K(+) and Anionic Currents in Intact Human Red Blood Cells
BACKGROUND: The mechanical, rheological and shape properties of red blood cells are determined by their cortical cytoskeleton, evolutionarily optimized to provide the dynamic deformability required for flow through capillaries much narrower than the cell's diameter. The shear stress induced by...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2829085/ https://www.ncbi.nlm.nih.gov/pubmed/20195477 http://dx.doi.org/10.1371/journal.pone.0009447 |
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author | Dyrda, Agnieszka Cytlak, Urszula Ciuraszkiewicz, Anna Lipinska, Agnieszka Cueff, Anne Bouyer, Guillaume Egée, Stéphane Bennekou, Poul Lew, Virgilio L. Thomas, Serge L. Y. |
author_facet | Dyrda, Agnieszka Cytlak, Urszula Ciuraszkiewicz, Anna Lipinska, Agnieszka Cueff, Anne Bouyer, Guillaume Egée, Stéphane Bennekou, Poul Lew, Virgilio L. Thomas, Serge L. Y. |
author_sort | Dyrda, Agnieszka |
collection | PubMed |
description | BACKGROUND: The mechanical, rheological and shape properties of red blood cells are determined by their cortical cytoskeleton, evolutionarily optimized to provide the dynamic deformability required for flow through capillaries much narrower than the cell's diameter. The shear stress induced by such flow, as well as the local membrane deformations generated in certain pathological conditions, such as sickle cell anemia, have been shown to increase membrane permeability, based largely on experimentation with red cell suspensions. We attempted here the first measurements of membrane currents activated by a local and controlled membrane deformation in single red blood cells under on-cell patch clamp to define the nature of the stretch-activated currents. METHODOLOGY/PRINCIPAL FINDINGS: The cell-attached configuration of the patch-clamp technique was used to allow recordings of single channel activity in intact red blood cells. Gigaohm seal formation was obtained with and without membrane deformation. Deformation was induced by the application of a negative pressure pulse of 10 mmHg for less than 5 s. Currents were only detected when the membrane was seen domed under negative pressure within the patch-pipette. K(+) and Cl(−) currents were strictly dependent on the presence of Ca(2+). The Ca(2+)-dependent currents were transient, with typical decay half-times of about 5–10 min, suggesting the spontaneous inactivation of a stretch-activated Ca(2+) permeability (PCa). These results indicate that local membrane deformations can transiently activate a Ca(2+) permeability pathway leading to increased [Ca(2+)](i), secondary activation of Ca(2+)-sensitive K(+) channels (Gardos channel, IK1, KCa3.1), and hyperpolarization-induced anion currents. CONCLUSIONS/SIGNIFICANCE: The stretch-activated transient PCa observed here under local membrane deformation is a likely contributor to the Ca(2+)-mediated effects observed during the normal aging process of red blood cells, and to the increased Ca(2+) content of red cells in certain hereditary anemias such as thalassemia and sickle cell anemia. |
format | Text |
id | pubmed-2829085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28290852010-03-02 Local Membrane Deformations Activate Ca(2+)-Dependent K(+) and Anionic Currents in Intact Human Red Blood Cells Dyrda, Agnieszka Cytlak, Urszula Ciuraszkiewicz, Anna Lipinska, Agnieszka Cueff, Anne Bouyer, Guillaume Egée, Stéphane Bennekou, Poul Lew, Virgilio L. Thomas, Serge L. Y. PLoS One Research Article BACKGROUND: The mechanical, rheological and shape properties of red blood cells are determined by their cortical cytoskeleton, evolutionarily optimized to provide the dynamic deformability required for flow through capillaries much narrower than the cell's diameter. The shear stress induced by such flow, as well as the local membrane deformations generated in certain pathological conditions, such as sickle cell anemia, have been shown to increase membrane permeability, based largely on experimentation with red cell suspensions. We attempted here the first measurements of membrane currents activated by a local and controlled membrane deformation in single red blood cells under on-cell patch clamp to define the nature of the stretch-activated currents. METHODOLOGY/PRINCIPAL FINDINGS: The cell-attached configuration of the patch-clamp technique was used to allow recordings of single channel activity in intact red blood cells. Gigaohm seal formation was obtained with and without membrane deformation. Deformation was induced by the application of a negative pressure pulse of 10 mmHg for less than 5 s. Currents were only detected when the membrane was seen domed under negative pressure within the patch-pipette. K(+) and Cl(−) currents were strictly dependent on the presence of Ca(2+). The Ca(2+)-dependent currents were transient, with typical decay half-times of about 5–10 min, suggesting the spontaneous inactivation of a stretch-activated Ca(2+) permeability (PCa). These results indicate that local membrane deformations can transiently activate a Ca(2+) permeability pathway leading to increased [Ca(2+)](i), secondary activation of Ca(2+)-sensitive K(+) channels (Gardos channel, IK1, KCa3.1), and hyperpolarization-induced anion currents. CONCLUSIONS/SIGNIFICANCE: The stretch-activated transient PCa observed here under local membrane deformation is a likely contributor to the Ca(2+)-mediated effects observed during the normal aging process of red blood cells, and to the increased Ca(2+) content of red cells in certain hereditary anemias such as thalassemia and sickle cell anemia. Public Library of Science 2010-02-26 /pmc/articles/PMC2829085/ /pubmed/20195477 http://dx.doi.org/10.1371/journal.pone.0009447 Text en Dyrda et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Dyrda, Agnieszka Cytlak, Urszula Ciuraszkiewicz, Anna Lipinska, Agnieszka Cueff, Anne Bouyer, Guillaume Egée, Stéphane Bennekou, Poul Lew, Virgilio L. Thomas, Serge L. Y. Local Membrane Deformations Activate Ca(2+)-Dependent K(+) and Anionic Currents in Intact Human Red Blood Cells |
title | Local Membrane Deformations Activate Ca(2+)-Dependent K(+) and Anionic Currents in Intact Human Red Blood Cells |
title_full | Local Membrane Deformations Activate Ca(2+)-Dependent K(+) and Anionic Currents in Intact Human Red Blood Cells |
title_fullStr | Local Membrane Deformations Activate Ca(2+)-Dependent K(+) and Anionic Currents in Intact Human Red Blood Cells |
title_full_unstemmed | Local Membrane Deformations Activate Ca(2+)-Dependent K(+) and Anionic Currents in Intact Human Red Blood Cells |
title_short | Local Membrane Deformations Activate Ca(2+)-Dependent K(+) and Anionic Currents in Intact Human Red Blood Cells |
title_sort | local membrane deformations activate ca(2+)-dependent k(+) and anionic currents in intact human red blood cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2829085/ https://www.ncbi.nlm.nih.gov/pubmed/20195477 http://dx.doi.org/10.1371/journal.pone.0009447 |
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