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Complete genome sequence of human astrovirus genotype 6
BACKGROUND: Human astroviruses (HAstVs) are one of the important causes of acute gastroenteritis in children. Currently, eight HAstV genotypes have been identified and all but two (HAstV-6 and HAstV-7) have been fully sequenced. We here sequenced and analyzed the complete genome of a HAstV-6 strain...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2829535/ https://www.ncbi.nlm.nih.gov/pubmed/20137100 http://dx.doi.org/10.1186/1743-422X-7-29 |
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author | Guo, Li Gonzalez, Richard Wang, Wei Li, Yongjun Paranhos-Baccalà, Gláucia Vernet, Guy Wang, Jianwei |
author_facet | Guo, Li Gonzalez, Richard Wang, Wei Li, Yongjun Paranhos-Baccalà, Gláucia Vernet, Guy Wang, Jianwei |
author_sort | Guo, Li |
collection | PubMed |
description | BACKGROUND: Human astroviruses (HAstVs) are one of the important causes of acute gastroenteritis in children. Currently, eight HAstV genotypes have been identified and all but two (HAstV-6 and HAstV-7) have been fully sequenced. We here sequenced and analyzed the complete genome of a HAstV-6 strain (192-BJ07), which was identified in Beijing, China. RESULTS: The genome of 192-BJ07 consists of 6745 nucleotides. The 192-BJ07 strain displays a 77.2-78.0% nucleotide sequence identity with other HAstV genotypes and exhibits amino acid sequence identities of 86.5-87.4%, 94.2-95.1%, and 65.5-74.8% in the ORF1a, ORF1b, and ORF2 regions, respectively. Homological analysis of ORF2 shows that 192-BJ07 is 96.3% identical to the documented HAstV-6 strain. Further, phylogenetic analysis indicates that different genomic regions are likely undergoing different evolutionary and selective pressures. No recombination event was observed in HAstV-6 in this study. CONCLUSION: The completely sequenced and characterized genome of HAstV-6 (192-BJ07) provides further insight into the genetics of astroviruses and aids in the surveillance and control of HAstV gastroenteritis. |
format | Text |
id | pubmed-2829535 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28295352010-02-28 Complete genome sequence of human astrovirus genotype 6 Guo, Li Gonzalez, Richard Wang, Wei Li, Yongjun Paranhos-Baccalà, Gláucia Vernet, Guy Wang, Jianwei Virol J Research BACKGROUND: Human astroviruses (HAstVs) are one of the important causes of acute gastroenteritis in children. Currently, eight HAstV genotypes have been identified and all but two (HAstV-6 and HAstV-7) have been fully sequenced. We here sequenced and analyzed the complete genome of a HAstV-6 strain (192-BJ07), which was identified in Beijing, China. RESULTS: The genome of 192-BJ07 consists of 6745 nucleotides. The 192-BJ07 strain displays a 77.2-78.0% nucleotide sequence identity with other HAstV genotypes and exhibits amino acid sequence identities of 86.5-87.4%, 94.2-95.1%, and 65.5-74.8% in the ORF1a, ORF1b, and ORF2 regions, respectively. Homological analysis of ORF2 shows that 192-BJ07 is 96.3% identical to the documented HAstV-6 strain. Further, phylogenetic analysis indicates that different genomic regions are likely undergoing different evolutionary and selective pressures. No recombination event was observed in HAstV-6 in this study. CONCLUSION: The completely sequenced and characterized genome of HAstV-6 (192-BJ07) provides further insight into the genetics of astroviruses and aids in the surveillance and control of HAstV gastroenteritis. BioMed Central 2010-02-08 /pmc/articles/PMC2829535/ /pubmed/20137100 http://dx.doi.org/10.1186/1743-422X-7-29 Text en Copyright ©2010 Guo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Guo, Li Gonzalez, Richard Wang, Wei Li, Yongjun Paranhos-Baccalà, Gláucia Vernet, Guy Wang, Jianwei Complete genome sequence of human astrovirus genotype 6 |
title | Complete genome sequence of human astrovirus genotype 6 |
title_full | Complete genome sequence of human astrovirus genotype 6 |
title_fullStr | Complete genome sequence of human astrovirus genotype 6 |
title_full_unstemmed | Complete genome sequence of human astrovirus genotype 6 |
title_short | Complete genome sequence of human astrovirus genotype 6 |
title_sort | complete genome sequence of human astrovirus genotype 6 |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2829535/ https://www.ncbi.nlm.nih.gov/pubmed/20137100 http://dx.doi.org/10.1186/1743-422X-7-29 |
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