Selection of bacteriophage λ integrases with altered recombination specificity by in vitro compartmentalization

In vitro compartmentalization (IVC) was employed for the first time to select for novel bacteriophage λ integrase variants displaying significantly enhanced recombination activity on a non-cognate target DNA sequence. These variants displayed up to 9-fold increased recombination activity over the pa...

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Detalles Bibliográficos
Autores principales: Tay, Yvonne, Ho, Candice, Drőge, Peter, Ghadessy, Farid J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831311/
https://www.ncbi.nlm.nih.gov/pubmed/19966270
http://dx.doi.org/10.1093/nar/gkp1089

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831311/
https://www.ncbi.nlm.nih.gov/pubmed/19966270
http://dx.doi.org/10.1093/nar/gkp1089

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