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Selection of bacteriophage λ integrases with altered recombination specificity by in vitro compartmentalization
In vitro compartmentalization (IVC) was employed for the first time to select for novel bacteriophage λ integrase variants displaying significantly enhanced recombination activity on a non-cognate target DNA sequence. These variants displayed up to 9-fold increased recombination activity over the pa...
Autores principales: | Tay, Yvonne, Ho, Candice, Drőge, Peter, Ghadessy, Farid J. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831311/ https://www.ncbi.nlm.nih.gov/pubmed/19966270 http://dx.doi.org/10.1093/nar/gkp1089 |
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