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Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs

Type IIS restriction endonuclease BtsCI (GGATG 2/0) is a neoschizomer of FokI (GGATG 9/13) and cleaves closer to the recognition sequence. Although M.BtsCI shows 62% amino acid sequence identity to M.FokI, BtsCI and FokI restriction endonucleases do not share significant amino acid sequence similari...

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Detalles Bibliográficos
Autores principales:	Too, Priscilla Hiu-Mei, Zhu, Zhenyu, Chan, Siu-Hong, Xu, Shuang-yong
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2010
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831314/ https://www.ncbi.nlm.nih.gov/pubmed/19955230 http://dx.doi.org/10.1093/nar/gkp1092

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