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A calibrated diversity assay for nucleic acid libraries using DiStRO—a Diversity Standard of Random Oligonucleotides
We have determined diversities exceeding 10(12) different sequences in an annealing and melting assay using synthetic randomized oligonucleotides as a standard. For such high diversities, the annealing kinetics differ from those observed for low diversities, favouring the remelting curve after annea...
| Autores principales: | , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831324/ https://www.ncbi.nlm.nih.gov/pubmed/19965765 http://dx.doi.org/10.1093/nar/gkp1108 |
| _version_ | 1782178238324277248 |
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| author | Schütze, Tatjana Arndt, Peter F. Menger, Marcus Wochner, Aniela Vingron, Martin Erdmann, Volker A. Lehrach, Hans Kaps, Christian Glökler, Jörn |
| author_facet | Schütze, Tatjana Arndt, Peter F. Menger, Marcus Wochner, Aniela Vingron, Martin Erdmann, Volker A. Lehrach, Hans Kaps, Christian Glökler, Jörn |
| author_sort | Schütze, Tatjana |
| collection | PubMed |
| description | We have determined diversities exceeding 10(12) different sequences in an annealing and melting assay using synthetic randomized oligonucleotides as a standard. For such high diversities, the annealing kinetics differ from those observed for low diversities, favouring the remelting curve after annealing as the best indicator of complexity. Direct comparisons of nucleic acid pools obtained from an aptamer selection demonstrate that even highly complex populations can be evaluated by using DiStRO, without the need of complicated calculations. |
| format | Text |
| id | pubmed-2831324 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2010 |
| publisher | Oxford University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-28313242010-03-03 A calibrated diversity assay for nucleic acid libraries using DiStRO—a Diversity Standard of Random Oligonucleotides Schütze, Tatjana Arndt, Peter F. Menger, Marcus Wochner, Aniela Vingron, Martin Erdmann, Volker A. Lehrach, Hans Kaps, Christian Glökler, Jörn Nucleic Acids Res Methods Online We have determined diversities exceeding 10(12) different sequences in an annealing and melting assay using synthetic randomized oligonucleotides as a standard. For such high diversities, the annealing kinetics differ from those observed for low diversities, favouring the remelting curve after annealing as the best indicator of complexity. Direct comparisons of nucleic acid pools obtained from an aptamer selection demonstrate that even highly complex populations can be evaluated by using DiStRO, without the need of complicated calculations. Oxford University Press 2010-03 2009-12-03 /pmc/articles/PMC2831324/ /pubmed/19965765 http://dx.doi.org/10.1093/nar/gkp1108 Text en © The Author(s) 2009. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Methods Online Schütze, Tatjana Arndt, Peter F. Menger, Marcus Wochner, Aniela Vingron, Martin Erdmann, Volker A. Lehrach, Hans Kaps, Christian Glökler, Jörn A calibrated diversity assay for nucleic acid libraries using DiStRO—a Diversity Standard of Random Oligonucleotides |
| title | A calibrated diversity assay for nucleic acid libraries using DiStRO—a Diversity Standard of Random Oligonucleotides |
| title_full | A calibrated diversity assay for nucleic acid libraries using DiStRO—a Diversity Standard of Random Oligonucleotides |
| title_fullStr | A calibrated diversity assay for nucleic acid libraries using DiStRO—a Diversity Standard of Random Oligonucleotides |
| title_full_unstemmed | A calibrated diversity assay for nucleic acid libraries using DiStRO—a Diversity Standard of Random Oligonucleotides |
| title_short | A calibrated diversity assay for nucleic acid libraries using DiStRO—a Diversity Standard of Random Oligonucleotides |
| title_sort | calibrated diversity assay for nucleic acid libraries using distro—a diversity standard of random oligonucleotides |
| topic | Methods Online |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831324/ https://www.ncbi.nlm.nih.gov/pubmed/19965765 http://dx.doi.org/10.1093/nar/gkp1108 |
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