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A calibrated diversity assay for nucleic acid libraries using DiStRO—a Diversity Standard of Random Oligonucleotides

We have determined diversities exceeding 10(12) different sequences in an annealing and melting assay using synthetic randomized oligonucleotides as a standard. For such high diversities, the annealing kinetics differ from those observed for low diversities, favouring the remelting curve after annea...

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Detalles Bibliográficos
Autores principales:	Schütze, Tatjana, Arndt, Peter F., Menger, Marcus, Wochner, Aniela, Vingron, Martin, Erdmann, Volker A., Lehrach, Hans, Kaps, Christian, Glökler, Jörn
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2010
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831324/ https://www.ncbi.nlm.nih.gov/pubmed/19965765 http://dx.doi.org/10.1093/nar/gkp1108

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