Cargando…

Gene Expression Profiling of Placentas Affected by Pre-Eclampsia

Several studies point to the placenta as the primary cause of pre-eclampsia. Our objective was to identify placental genes that may contribute to the development of pre-eclampsia. RNA was purified from tissue biopsies from eleven pre-eclamptic placentas and eighteen normal controls. Messenger RNA ex...

Descripción completa

Detalles Bibliográficos
Autores principales: Hoegh, Anne Mette, Borup, Rehannah, Nielsen, Finn Cilius, Sørensen, Steen, Hviid, Thomas V. F.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831461/
https://www.ncbi.nlm.nih.gov/pubmed/20204130
http://dx.doi.org/10.1155/2010/787545
_version_ 1782178243014557696
author Hoegh, Anne Mette
Borup, Rehannah
Nielsen, Finn Cilius
Sørensen, Steen
Hviid, Thomas V. F.
author_facet Hoegh, Anne Mette
Borup, Rehannah
Nielsen, Finn Cilius
Sørensen, Steen
Hviid, Thomas V. F.
author_sort Hoegh, Anne Mette
collection PubMed
description Several studies point to the placenta as the primary cause of pre-eclampsia. Our objective was to identify placental genes that may contribute to the development of pre-eclampsia. RNA was purified from tissue biopsies from eleven pre-eclamptic placentas and eighteen normal controls. Messenger RNA expression from pooled samples was analysed by microarrays. Verification of the expression of selected genes was performed using real-time PCR. A surprisingly low number of genes (21 out of 15,000) were identified as differentially expressed. Among these were genes not previously associated with pre-eclampsia as bradykinin B1 receptor and a 14-3-3 protein, but also genes that have already been connected with pre-eclampsia, for example, inhibin beta A subunit and leptin. A low number of genes were repeatedly identified as differentially expressed, because they may represent the endpoint of a cascade of events effectuated throughout gestation. They were associated with transcriptional regulation and vasoregulative pathways, along with a number of hypothetical proteins and gene sequences with unknown functions.
format Text
id pubmed-2831461
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-28314612010-03-04 Gene Expression Profiling of Placentas Affected by Pre-Eclampsia Hoegh, Anne Mette Borup, Rehannah Nielsen, Finn Cilius Sørensen, Steen Hviid, Thomas V. F. J Biomed Biotechnol Research Article Several studies point to the placenta as the primary cause of pre-eclampsia. Our objective was to identify placental genes that may contribute to the development of pre-eclampsia. RNA was purified from tissue biopsies from eleven pre-eclamptic placentas and eighteen normal controls. Messenger RNA expression from pooled samples was analysed by microarrays. Verification of the expression of selected genes was performed using real-time PCR. A surprisingly low number of genes (21 out of 15,000) were identified as differentially expressed. Among these were genes not previously associated with pre-eclampsia as bradykinin B1 receptor and a 14-3-3 protein, but also genes that have already been connected with pre-eclampsia, for example, inhibin beta A subunit and leptin. A low number of genes were repeatedly identified as differentially expressed, because they may represent the endpoint of a cascade of events effectuated throughout gestation. They were associated with transcriptional regulation and vasoregulative pathways, along with a number of hypothetical proteins and gene sequences with unknown functions. Hindawi Publishing Corporation 2010 2010-03-03 /pmc/articles/PMC2831461/ /pubmed/20204130 http://dx.doi.org/10.1155/2010/787545 Text en Copyright © 2010 Anne Mette Hoegh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hoegh, Anne Mette
Borup, Rehannah
Nielsen, Finn Cilius
Sørensen, Steen
Hviid, Thomas V. F.
Gene Expression Profiling of Placentas Affected by Pre-Eclampsia
title Gene Expression Profiling of Placentas Affected by Pre-Eclampsia
title_full Gene Expression Profiling of Placentas Affected by Pre-Eclampsia
title_fullStr Gene Expression Profiling of Placentas Affected by Pre-Eclampsia
title_full_unstemmed Gene Expression Profiling of Placentas Affected by Pre-Eclampsia
title_short Gene Expression Profiling of Placentas Affected by Pre-Eclampsia
title_sort gene expression profiling of placentas affected by pre-eclampsia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831461/
https://www.ncbi.nlm.nih.gov/pubmed/20204130
http://dx.doi.org/10.1155/2010/787545
work_keys_str_mv AT hoeghannemette geneexpressionprofilingofplacentasaffectedbypreeclampsia
AT boruprehannah geneexpressionprofilingofplacentasaffectedbypreeclampsia
AT nielsenfinncilius geneexpressionprofilingofplacentasaffectedbypreeclampsia
AT sørensensteen geneexpressionprofilingofplacentasaffectedbypreeclampsia
AT hviidthomasvf geneexpressionprofilingofplacentasaffectedbypreeclampsia