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Gene Expression Profiling of Placentas Affected by Pre-Eclampsia
Several studies point to the placenta as the primary cause of pre-eclampsia. Our objective was to identify placental genes that may contribute to the development of pre-eclampsia. RNA was purified from tissue biopsies from eleven pre-eclamptic placentas and eighteen normal controls. Messenger RNA ex...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831461/ https://www.ncbi.nlm.nih.gov/pubmed/20204130 http://dx.doi.org/10.1155/2010/787545 |
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author | Hoegh, Anne Mette Borup, Rehannah Nielsen, Finn Cilius Sørensen, Steen Hviid, Thomas V. F. |
author_facet | Hoegh, Anne Mette Borup, Rehannah Nielsen, Finn Cilius Sørensen, Steen Hviid, Thomas V. F. |
author_sort | Hoegh, Anne Mette |
collection | PubMed |
description | Several studies point to the placenta as the primary cause of pre-eclampsia. Our objective was to identify placental genes that may contribute to the development of pre-eclampsia. RNA was purified from tissue biopsies from eleven pre-eclamptic placentas and eighteen normal controls. Messenger RNA expression from pooled samples was analysed by microarrays. Verification of the expression of selected genes was performed using real-time PCR. A surprisingly low number of genes (21 out of 15,000) were identified as differentially expressed. Among these were genes not previously associated with pre-eclampsia as bradykinin B1 receptor and a 14-3-3 protein, but also genes that have already been connected with pre-eclampsia, for example, inhibin beta A subunit and leptin. A low number of genes were repeatedly identified as differentially expressed, because they may represent the endpoint of a cascade of events effectuated throughout gestation. They were associated with transcriptional regulation and vasoregulative pathways, along with a number of hypothetical proteins and gene sequences with unknown functions. |
format | Text |
id | pubmed-2831461 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-28314612010-03-04 Gene Expression Profiling of Placentas Affected by Pre-Eclampsia Hoegh, Anne Mette Borup, Rehannah Nielsen, Finn Cilius Sørensen, Steen Hviid, Thomas V. F. J Biomed Biotechnol Research Article Several studies point to the placenta as the primary cause of pre-eclampsia. Our objective was to identify placental genes that may contribute to the development of pre-eclampsia. RNA was purified from tissue biopsies from eleven pre-eclamptic placentas and eighteen normal controls. Messenger RNA expression from pooled samples was analysed by microarrays. Verification of the expression of selected genes was performed using real-time PCR. A surprisingly low number of genes (21 out of 15,000) were identified as differentially expressed. Among these were genes not previously associated with pre-eclampsia as bradykinin B1 receptor and a 14-3-3 protein, but also genes that have already been connected with pre-eclampsia, for example, inhibin beta A subunit and leptin. A low number of genes were repeatedly identified as differentially expressed, because they may represent the endpoint of a cascade of events effectuated throughout gestation. They were associated with transcriptional regulation and vasoregulative pathways, along with a number of hypothetical proteins and gene sequences with unknown functions. Hindawi Publishing Corporation 2010 2010-03-03 /pmc/articles/PMC2831461/ /pubmed/20204130 http://dx.doi.org/10.1155/2010/787545 Text en Copyright © 2010 Anne Mette Hoegh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hoegh, Anne Mette Borup, Rehannah Nielsen, Finn Cilius Sørensen, Steen Hviid, Thomas V. F. Gene Expression Profiling of Placentas Affected by Pre-Eclampsia |
title | Gene Expression Profiling of Placentas Affected by Pre-Eclampsia |
title_full | Gene Expression Profiling of Placentas Affected by Pre-Eclampsia |
title_fullStr | Gene Expression Profiling of Placentas Affected by Pre-Eclampsia |
title_full_unstemmed | Gene Expression Profiling of Placentas Affected by Pre-Eclampsia |
title_short | Gene Expression Profiling of Placentas Affected by Pre-Eclampsia |
title_sort | gene expression profiling of placentas affected by pre-eclampsia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831461/ https://www.ncbi.nlm.nih.gov/pubmed/20204130 http://dx.doi.org/10.1155/2010/787545 |
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