Cargando…

High-level expression and purification of soluble recombinant FGF21 protein by SUMO fusion in Escherichia coli

BACKGROUND: Fibroblast growth factor 21 (FGF21) is a promising drug candidate to combat metabolic diseases. However, high-level expression and purification of recombinant FGF21 (rFGF21) in Escherichia coli (E. coli) is difficult because rFGF21 forms inclusion bodies in the bacteria making it difficu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Huiyan, Xiao, Yechen, Fu, Lianjun, Zhao, Hongxin, Zhang, Yaofang, Wan, Xiaoshan, Qin, Yuxia, Huang, Yadong, Gao, Hongchang, Li, Xiaokun
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Research article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831817/ https://www.ncbi.nlm.nih.gov/pubmed/20163718 http://dx.doi.org/10.1186/1472-6750-10-14

Ejemplares similares

Soluble cytoplasmic expression, rapid purification, and characterization of cyanovirin-N as a His-SUMO fusion
por: Gao, Xianglei, et al.
Publicado: (2009)

Expression and Purification of Human Keratinocyte Growth Factor 2 by Fusion with SUMO
por: Wu, Xiaoping, et al.
Publicado: (2008)

Enhanced Expression and Purification of Membrane Proteins by SUMO Fusion in Escherichia coli
por: Zuo, Xun, et al.
Publicado: (2005)

SUMO fusion system facilitates soluble expression and high production of bioactive human fibroblast growth factor 23 (FGF23)
por: Liu, Xiaoju, et al.
Publicado: (2012)

A Better Anti-Diabetic Recombinant Human Fibroblast Growth Factor 21 (rhFGF21) Modified with Polyethylene Glycol
por: Huang, Zhifeng, et al.
Publicado: (2011)

Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO
por: Marblestone, Jeffrey G., et al.
Publicado: (2006)

Cloning, Soluble Expression and Purification of High Yield Recombinant hGMCSF in Escherichia coli
por: Das, Krishna M.P., et al.
Publicado: (2011)

Expression, Purification, and Bioactivity of a Soluble Recombinant Ovine Interferon-tau in Escherichia Coli
por: Yu, Hai-Yang, et al.
Publicado: (2021)

Expression and purification of SARS coronavirus proteins using SUMO-fusions
por: Zuo, Xun, et al.
Publicado: (2005)

Potent long-acting rhFGF21 analog for treatment of diabetic nephropathy in db/db and DIO mice
por: Zhao, Longwei, et al.
Publicado: (2017)

Expression and purification of functional HMGB1 A box by fusion with SUMO
por: GE, WEN-SONG, et al.
Publicado: (2015)

SUMO Wrestles with Recombination
por: Altmannová, Veronika, et al.
Publicado: (2012)

Cell-penetrating peptide TAT-mediated delivery of acidic FGF to retina and protection against ischemia–reperfusion injury in rats
por: Wang, Yi, et al.
Publicado: (2010)

Highly efficient soluble expression, purification and characterization of recombinant Aβ42 from Escherichia coli
por: Jia, Longgang, et al.
Publicado: (2018)

Expression and Purification of Recombinant Hemoglobin in Escherichia coli
por: Natarajan, Chandrasekhar, et al.
Publicado: (2011)

Soluble Expression of Disulfide Bond Containing Proteins FGF15 and FGF19 in the Cytoplasm of Escherichia coli
por: Kong, Bo, et al.
Publicado: (2014)

Cloning, expression and purification of recombinant dermatopontin in Escherichia coli
por: Seetaraman Amritha, Trikkur Madom, et al.
Publicado: (2020)

Differential Specificity of Endocrine FGF19 and FGF21 to FGFR1 and FGFR4 in Complex with KLB
por: Yang, Chaofeng, et al.
Publicado: (2012)

Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system
por: Costa, Sofia, et al.
Publicado: (2014)

Expression, purification and characterization of soluble red rooster laforin as a fusion protein in Escherichia coli
por: Brewer, M Kathryn, et al.
Publicado: (2014)

Physiological and Pharmacological Roles of FGF21 in Cardiovascular Diseases
por: Cheng, Peng, et al.
Publicado: (2016)

Fusion Tag Design Influences Soluble Recombinant Protein Production in Escherichia coli
por: Köppl, Christoph, et al.
Publicado: (2022)

Enhanced soluble expression of active recombinant human interleukin-29 using champion pET SUMO system
por: Munir, Ayesha, et al.
Publicado: (2023)

FGF19 subfamily members: FGF19 and FGF21
por: Dolegowska, Katarzyna, et al.
Publicado: (2019)

Agonistic β-Klotho antibody mimics fibroblast growth factor 21 (FGF21) functions
por: Min, Xiaoshan, et al.
Publicado: (2018)

Heating-mediated purification of active FGF21 and structure-based design of its variant with enhanced potency
por: Jung, Ye-Eun, et al.
Publicado: (2023)

Expression and purification of soluble recombinant β-lactamases using Escherichia coli as expression host and pET-28a as cloning vector
por: Li, Lele, et al.
Publicado: (2022)

Cloning, Optimization of Periplasmic Expression and Purification of Recombinant Granulocyte Macrophage-Stimulating Factor in Escherichia coli BL21 (DE3)
por: Taherian, Elham, et al.
Publicado: (2019)

Expression of soluble native protein in Escherichia coli using a cold-shock SUMO tag-fused expression vector
por: Li, Jianghui, et al.
Publicado: (2018)

Soluble Expression and Efficient Purification of Recombinant Class I Hydrophobin DewA
por: Ahn, Sang-Oh, et al.
Publicado: (2021)

Expression and purification of soluble and active human enterokinase light chain in Escherichia coli
por: Kim, Young Su, et al.
Publicado: (2021)

Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli
por: Sørensen, Hans Peter, et al.
Publicado: (2005)

A simple and rapid protein purification method based on cell-surface display of SUMO-fused recombinant protein and Ulp1 protease
por: Zhou, Xiao-Feng, et al.
Publicado: (2020)

Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
por: MacEwan, Sarah R., et al.
Publicado: (2014)

Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification
por: Shankar, Ram, et al.
Publicado: (2021)

Intricate SUMO-based control of the homologous recombination machinery
por: Dhingra, Nalini, et al.
Publicado: (2019)

FGF19 and FGF21: In NASH we trust
por: Talukdar, Saswata, et al.
Publicado: (2020)

The FGF metabolic axis
por: Li, Xiaokun
Publicado: (2019)

High-level Expression and Purification of Active Human FGF-2 in Escherichia coli by Codon and Culture Condition Optimization
por: Soleyman, Mohammad Reza, et al.
Publicado: (2016)

High-Efficiency Expression of TAT-bFGF Fusion Protein in Escherichia coli and the Effect on Hypertrophic Scar Tissue
por: Jia, Xuechao, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_904oqvnurkpt13g5j7t93voa4h