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Sp1 and KLF15 regulate basal transcription of the human LRP5 gene
BACKGROUND: LRP5, a member of the low density lipoprotein receptor superfamily, regulates diverse developmental processes in embryogenesis and maintains physiological homeostasis in adult organisms. However, how the expression of human LRP5 gene is regulated remains unclear. RESULTS: In order to cha...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831824/ https://www.ncbi.nlm.nih.gov/pubmed/20141633 http://dx.doi.org/10.1186/1471-2156-11-12 |
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author | Li, Jiangxia Yang, Yang Jiang, Baichun Zhang, Xiyu Zou, Yongxin Gong, Yaoqin |
author_facet | Li, Jiangxia Yang, Yang Jiang, Baichun Zhang, Xiyu Zou, Yongxin Gong, Yaoqin |
author_sort | Li, Jiangxia |
collection | PubMed |
description | BACKGROUND: LRP5, a member of the low density lipoprotein receptor superfamily, regulates diverse developmental processes in embryogenesis and maintains physiological homeostasis in adult organisms. However, how the expression of human LRP5 gene is regulated remains unclear. RESULTS: In order to characterize the transcriptional regulation of human LRP5 gene, we cloned the 5' flanking region and evaluated its transcriptional activity in a luciferase reporter system. We demonstrated that both KLF15 and Sp1 binding sites between -72 bp and -53 bp contribute to the transcriptional activation of human LRP5 promoter. Chromatin immunoprecipitation assay demonstrated that the ubiquitous transcription factors KLF15 and Sp1 bind to this region. Using Drosophila SL2 cells, we showed that KLF15 and Sp1 trans-activated the LRP5 promoter in a manner dependent on the presence of Sp1-binding and KLF15-binding motifs. CONCLUSIONS: Both KLF15 and Sp1 binding sites contribute to the basal activity of human LRP5 promoter. This study provides the first insight into the mechanisms by which transcription of human LRP5 gene is regulated. |
format | Text |
id | pubmed-2831824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28318242010-03-04 Sp1 and KLF15 regulate basal transcription of the human LRP5 gene Li, Jiangxia Yang, Yang Jiang, Baichun Zhang, Xiyu Zou, Yongxin Gong, Yaoqin BMC Genet Research article BACKGROUND: LRP5, a member of the low density lipoprotein receptor superfamily, regulates diverse developmental processes in embryogenesis and maintains physiological homeostasis in adult organisms. However, how the expression of human LRP5 gene is regulated remains unclear. RESULTS: In order to characterize the transcriptional regulation of human LRP5 gene, we cloned the 5' flanking region and evaluated its transcriptional activity in a luciferase reporter system. We demonstrated that both KLF15 and Sp1 binding sites between -72 bp and -53 bp contribute to the transcriptional activation of human LRP5 promoter. Chromatin immunoprecipitation assay demonstrated that the ubiquitous transcription factors KLF15 and Sp1 bind to this region. Using Drosophila SL2 cells, we showed that KLF15 and Sp1 trans-activated the LRP5 promoter in a manner dependent on the presence of Sp1-binding and KLF15-binding motifs. CONCLUSIONS: Both KLF15 and Sp1 binding sites contribute to the basal activity of human LRP5 promoter. This study provides the first insight into the mechanisms by which transcription of human LRP5 gene is regulated. BioMed Central 2010-02-08 /pmc/articles/PMC2831824/ /pubmed/20141633 http://dx.doi.org/10.1186/1471-2156-11-12 Text en Copyright ©2010 Li et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research article Li, Jiangxia Yang, Yang Jiang, Baichun Zhang, Xiyu Zou, Yongxin Gong, Yaoqin Sp1 and KLF15 regulate basal transcription of the human LRP5 gene |
title | Sp1 and KLF15 regulate basal transcription of the human LRP5 gene |
title_full | Sp1 and KLF15 regulate basal transcription of the human LRP5 gene |
title_fullStr | Sp1 and KLF15 regulate basal transcription of the human LRP5 gene |
title_full_unstemmed | Sp1 and KLF15 regulate basal transcription of the human LRP5 gene |
title_short | Sp1 and KLF15 regulate basal transcription of the human LRP5 gene |
title_sort | sp1 and klf15 regulate basal transcription of the human lrp5 gene |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2831824/ https://www.ncbi.nlm.nih.gov/pubmed/20141633 http://dx.doi.org/10.1186/1471-2156-11-12 |
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