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Genome Sequence of Cronobacter sakazakii BAA-894 and Comparative Genomic Hybridization Analysis with Other Cronobacter Species

BACKGROUND: The genus Cronobacter (formerly called Enterobacter sakazakii) is composed of five species; C. sakazakii, C. malonaticus, C. turicensis, C. muytjensii, and C. dublinensis. The genus includes opportunistic human pathogens, and the first three species have been associated with neonatal inf...

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Autores principales: Kucerova, Eva, Clifton, Sandra W., Xia, Xiao-Qin, Long, Fred, Porwollik, Steffen, Fulton, Lucinda, Fronick, Catrina, Minx, Patrick, Kyung, Kim, Warren, Wesley, Fulton, Robert, Feng, Dongyan, Wollam, Aye, Shah, Neha, Bhonagiri, Veena, Nash, William E., Hallsworth-Pepin, Kymberlie, Wilson, Richard K., McClelland, Michael, Forsythe, Stephen J.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2833190/
https://www.ncbi.nlm.nih.gov/pubmed/20221447
http://dx.doi.org/10.1371/journal.pone.0009556
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author Kucerova, Eva
Clifton, Sandra W.
Xia, Xiao-Qin
Long, Fred
Porwollik, Steffen
Fulton, Lucinda
Fronick, Catrina
Minx, Patrick
Kyung, Kim
Warren, Wesley
Fulton, Robert
Feng, Dongyan
Wollam, Aye
Shah, Neha
Bhonagiri, Veena
Nash, William E.
Hallsworth-Pepin, Kymberlie
Wilson, Richard K.
McClelland, Michael
Forsythe, Stephen J.
author_facet Kucerova, Eva
Clifton, Sandra W.
Xia, Xiao-Qin
Long, Fred
Porwollik, Steffen
Fulton, Lucinda
Fronick, Catrina
Minx, Patrick
Kyung, Kim
Warren, Wesley
Fulton, Robert
Feng, Dongyan
Wollam, Aye
Shah, Neha
Bhonagiri, Veena
Nash, William E.
Hallsworth-Pepin, Kymberlie
Wilson, Richard K.
McClelland, Michael
Forsythe, Stephen J.
author_sort Kucerova, Eva
collection PubMed
description BACKGROUND: The genus Cronobacter (formerly called Enterobacter sakazakii) is composed of five species; C. sakazakii, C. malonaticus, C. turicensis, C. muytjensii, and C. dublinensis. The genus includes opportunistic human pathogens, and the first three species have been associated with neonatal infections. The most severe diseases are caused in neonates and include fatal necrotizing enterocolitis and meningitis. The genetic basis of the diversity within the genus is unknown, and few virulence traits have been identified. METHODOLOGY/PRINCIPAL FINDINGS: We report here the first sequence of a member of this genus, C. sakazakii strain BAA-894. The genome of Cronobacter sakazakii strain BAA-894 comprises a 4.4 Mb chromosome (57% GC content) and two plasmids; 31 kb (51% GC) and 131 kb (56% GC). The genome was used to construct a 387,000 probe oligonucleotide tiling DNA microarray covering the whole genome. Comparative genomic hybridization (CGH) was undertaken on five other C. sakazakii strains, and representatives of the four other Cronobacter species. Among 4,382 annotated genes inspected in this study, about 55% of genes were common to all C. sakazakii strains and 43% were common to all Cronobacter strains, with 10–17% absence of genes. CONCLUSIONS/SIGNIFICANCE: CGH highlighted 15 clusters of genes in C. sakazakii BAA-894 that were divergent or absent in more than half of the tested strains; six of these are of probable prophage origin. Putative virulence factors were identified in these prophage and in other variable regions. A number of genes unique to Cronobacter species associated with neonatal infections (C. sakazakii, C. malonaticus and C. turicensis) were identified. These included a copper and silver resistance system known to be linked to invasion of the blood-brain barrier by neonatal meningitic strains of Escherichia coli. In addition, genes encoding for multidrug efflux pumps and adhesins were identified that were unique to C. sakazakii strains from outbreaks in neonatal intensive care units.
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spelling pubmed-28331902010-03-11 Genome Sequence of Cronobacter sakazakii BAA-894 and Comparative Genomic Hybridization Analysis with Other Cronobacter Species Kucerova, Eva Clifton, Sandra W. Xia, Xiao-Qin Long, Fred Porwollik, Steffen Fulton, Lucinda Fronick, Catrina Minx, Patrick Kyung, Kim Warren, Wesley Fulton, Robert Feng, Dongyan Wollam, Aye Shah, Neha Bhonagiri, Veena Nash, William E. Hallsworth-Pepin, Kymberlie Wilson, Richard K. McClelland, Michael Forsythe, Stephen J. PLoS One Research Article BACKGROUND: The genus Cronobacter (formerly called Enterobacter sakazakii) is composed of five species; C. sakazakii, C. malonaticus, C. turicensis, C. muytjensii, and C. dublinensis. The genus includes opportunistic human pathogens, and the first three species have been associated with neonatal infections. The most severe diseases are caused in neonates and include fatal necrotizing enterocolitis and meningitis. The genetic basis of the diversity within the genus is unknown, and few virulence traits have been identified. METHODOLOGY/PRINCIPAL FINDINGS: We report here the first sequence of a member of this genus, C. sakazakii strain BAA-894. The genome of Cronobacter sakazakii strain BAA-894 comprises a 4.4 Mb chromosome (57% GC content) and two plasmids; 31 kb (51% GC) and 131 kb (56% GC). The genome was used to construct a 387,000 probe oligonucleotide tiling DNA microarray covering the whole genome. Comparative genomic hybridization (CGH) was undertaken on five other C. sakazakii strains, and representatives of the four other Cronobacter species. Among 4,382 annotated genes inspected in this study, about 55% of genes were common to all C. sakazakii strains and 43% were common to all Cronobacter strains, with 10–17% absence of genes. CONCLUSIONS/SIGNIFICANCE: CGH highlighted 15 clusters of genes in C. sakazakii BAA-894 that were divergent or absent in more than half of the tested strains; six of these are of probable prophage origin. Putative virulence factors were identified in these prophage and in other variable regions. A number of genes unique to Cronobacter species associated with neonatal infections (C. sakazakii, C. malonaticus and C. turicensis) were identified. These included a copper and silver resistance system known to be linked to invasion of the blood-brain barrier by neonatal meningitic strains of Escherichia coli. In addition, genes encoding for multidrug efflux pumps and adhesins were identified that were unique to C. sakazakii strains from outbreaks in neonatal intensive care units. Public Library of Science 2010-03-08 /pmc/articles/PMC2833190/ /pubmed/20221447 http://dx.doi.org/10.1371/journal.pone.0009556 Text en Kucerova et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kucerova, Eva
Clifton, Sandra W.
Xia, Xiao-Qin
Long, Fred
Porwollik, Steffen
Fulton, Lucinda
Fronick, Catrina
Minx, Patrick
Kyung, Kim
Warren, Wesley
Fulton, Robert
Feng, Dongyan
Wollam, Aye
Shah, Neha
Bhonagiri, Veena
Nash, William E.
Hallsworth-Pepin, Kymberlie
Wilson, Richard K.
McClelland, Michael
Forsythe, Stephen J.
Genome Sequence of Cronobacter sakazakii BAA-894 and Comparative Genomic Hybridization Analysis with Other Cronobacter Species
title Genome Sequence of Cronobacter sakazakii BAA-894 and Comparative Genomic Hybridization Analysis with Other Cronobacter Species
title_full Genome Sequence of Cronobacter sakazakii BAA-894 and Comparative Genomic Hybridization Analysis with Other Cronobacter Species
title_fullStr Genome Sequence of Cronobacter sakazakii BAA-894 and Comparative Genomic Hybridization Analysis with Other Cronobacter Species
title_full_unstemmed Genome Sequence of Cronobacter sakazakii BAA-894 and Comparative Genomic Hybridization Analysis with Other Cronobacter Species
title_short Genome Sequence of Cronobacter sakazakii BAA-894 and Comparative Genomic Hybridization Analysis with Other Cronobacter Species
title_sort genome sequence of cronobacter sakazakii baa-894 and comparative genomic hybridization analysis with other cronobacter species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2833190/
https://www.ncbi.nlm.nih.gov/pubmed/20221447
http://dx.doi.org/10.1371/journal.pone.0009556
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