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The Adenovirus Type 3 Dodecahedron's RGD Loop Comprises an HSPG Binding Site That Influences Integrin Binding

Human type 3 adenovirus dodecahedron (a virus like particle made of twelve penton bases) features the ability to enter cells through Heparan Sulphate Proteoglycans (HSPGs) and integrins interaction and is used as a versatile vector to deliver DNA or proteins. Cryo-EM reconstruction of the pseudovira...

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Autores principales: Gout, E., Schoehn, G., Fenel, D., Lortat-Jacob, H., Fender, P.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2833306/
https://www.ncbi.nlm.nih.gov/pubmed/20224646
http://dx.doi.org/10.1155/2010/541939
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author Gout, E.
Schoehn, G.
Fenel, D.
Lortat-Jacob, H.
Fender, P.
author_facet Gout, E.
Schoehn, G.
Fenel, D.
Lortat-Jacob, H.
Fender, P.
author_sort Gout, E.
collection PubMed
description Human type 3 adenovirus dodecahedron (a virus like particle made of twelve penton bases) features the ability to enter cells through Heparan Sulphate Proteoglycans (HSPGs) and integrins interaction and is used as a versatile vector to deliver DNA or proteins. Cryo-EM reconstruction of the pseudoviral particle with Heparan Sulphate (HS) oligosaccharide shows an extradensity on the RGD loop. A set of mutants was designed to study the respective roles of the RGD sequence (RGE mutant) and of a basic sequence located just downstream. Results showed that the RGE mutant binding to the HS deficient CHO-2241 cells was abolished and unexpectedly, mutation of the basic sequence (KQKR to AQAS) dramatically decreased integrin recognition by the viral pseudoparticle. This basic sequence is thus involved in integrin docking, showing a close interplay between HSPGs and integrin receptors.
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spelling pubmed-28333062010-03-11 The Adenovirus Type 3 Dodecahedron's RGD Loop Comprises an HSPG Binding Site That Influences Integrin Binding Gout, E. Schoehn, G. Fenel, D. Lortat-Jacob, H. Fender, P. J Biomed Biotechnol Research Article Human type 3 adenovirus dodecahedron (a virus like particle made of twelve penton bases) features the ability to enter cells through Heparan Sulphate Proteoglycans (HSPGs) and integrins interaction and is used as a versatile vector to deliver DNA or proteins. Cryo-EM reconstruction of the pseudoviral particle with Heparan Sulphate (HS) oligosaccharide shows an extradensity on the RGD loop. A set of mutants was designed to study the respective roles of the RGD sequence (RGE mutant) and of a basic sequence located just downstream. Results showed that the RGE mutant binding to the HS deficient CHO-2241 cells was abolished and unexpectedly, mutation of the basic sequence (KQKR to AQAS) dramatically decreased integrin recognition by the viral pseudoparticle. This basic sequence is thus involved in integrin docking, showing a close interplay between HSPGs and integrin receptors. Hindawi Publishing Corporation 2010 2010-03-04 /pmc/articles/PMC2833306/ /pubmed/20224646 http://dx.doi.org/10.1155/2010/541939 Text en Copyright © 2010 E. Gout et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gout, E.
Schoehn, G.
Fenel, D.
Lortat-Jacob, H.
Fender, P.
The Adenovirus Type 3 Dodecahedron's RGD Loop Comprises an HSPG Binding Site That Influences Integrin Binding
title The Adenovirus Type 3 Dodecahedron's RGD Loop Comprises an HSPG Binding Site That Influences Integrin Binding
title_full The Adenovirus Type 3 Dodecahedron's RGD Loop Comprises an HSPG Binding Site That Influences Integrin Binding
title_fullStr The Adenovirus Type 3 Dodecahedron's RGD Loop Comprises an HSPG Binding Site That Influences Integrin Binding
title_full_unstemmed The Adenovirus Type 3 Dodecahedron's RGD Loop Comprises an HSPG Binding Site That Influences Integrin Binding
title_short The Adenovirus Type 3 Dodecahedron's RGD Loop Comprises an HSPG Binding Site That Influences Integrin Binding
title_sort adenovirus type 3 dodecahedron's rgd loop comprises an hspg binding site that influences integrin binding
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2833306/
https://www.ncbi.nlm.nih.gov/pubmed/20224646
http://dx.doi.org/10.1155/2010/541939
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