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Mosaic Vaccines Elicit CD8+ T lymphocyte Responses in Monkeys that Confer Enhanced Immune Coverage of Diverse HIV Strains

An effective HIV vaccine must elicit immune responses that recognize genetically diverse viruses1, 2. It must generate CD8+ T lymphocytes that control HIV replication and CD4+ T lymphocytes that provide help for the generation and maintenance of both cellular and humoral immune responses against the...

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Autores principales: Santra, Sampa, Liao, Hua-Xin, Zhang, Ruijin, Muldoon, Mark, Watson, Sydeaka, Fischer, Will, Theiler, James, Szinger, James, Balachandran, Harikrishnan, Buzby, Adam, Quinn, David, Parks, Robert J., Tsao, Chun-Yen, Carville, Angela, Mansfield, Keith G., Pavlakis, George N., Felber, Barbara K., Haynes, Barton F., Korber, Bette T., Letvin, Norman L.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2834806/
https://www.ncbi.nlm.nih.gov/pubmed/20173754
http://dx.doi.org/10.1038/nm.2108
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author Santra, Sampa
Liao, Hua-Xin
Zhang, Ruijin
Muldoon, Mark
Watson, Sydeaka
Fischer, Will
Theiler, James
Szinger, James
Balachandran, Harikrishnan
Buzby, Adam
Quinn, David
Parks, Robert J.
Tsao, Chun-Yen
Carville, Angela
Mansfield, Keith G.
Pavlakis, George N.
Felber, Barbara K.
Haynes, Barton F.
Korber, Bette T.
Letvin, Norman L.
author_facet Santra, Sampa
Liao, Hua-Xin
Zhang, Ruijin
Muldoon, Mark
Watson, Sydeaka
Fischer, Will
Theiler, James
Szinger, James
Balachandran, Harikrishnan
Buzby, Adam
Quinn, David
Parks, Robert J.
Tsao, Chun-Yen
Carville, Angela
Mansfield, Keith G.
Pavlakis, George N.
Felber, Barbara K.
Haynes, Barton F.
Korber, Bette T.
Letvin, Norman L.
author_sort Santra, Sampa
collection PubMed
description An effective HIV vaccine must elicit immune responses that recognize genetically diverse viruses1, 2. It must generate CD8+ T lymphocytes that control HIV replication and CD4+ T lymphocytes that provide help for the generation and maintenance of both cellular and humoral immune responses against the virus3–5. Creating immunogens that can elicit cellular immune responses against the genetically varied circulating isolates of HIV presents an important challenge for creating an AIDS vaccine6, 7. Polyvalent mosaic immunogens derived by in silico recombination of natural strains of HIV are designed to induce cellular immune responses that recognize genetically diverse circulating virus isolates8. In the present study we immunized rhesus monkeys by plasmid DNA prime/ recombinant vaccinia virus boost using vaccine constructs expressing either consensus or polyvalent mosaic proteins. The mosaic immunogens elicited CD8+ T lymphocyte responses to more epitopes of each viral protein than the consensus immunogens, and to more variant sequences of CD8+ T lymphocyte epitopes. This increased breadth and depth of epitope recognition may contribute both to protection against infection by genetically diverse viruses and to the control of variant viruses that emerge as they mutate away from recognition by cytotoxic T lymphocytes.
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spelling pubmed-28348062010-09-01 Mosaic Vaccines Elicit CD8+ T lymphocyte Responses in Monkeys that Confer Enhanced Immune Coverage of Diverse HIV Strains Santra, Sampa Liao, Hua-Xin Zhang, Ruijin Muldoon, Mark Watson, Sydeaka Fischer, Will Theiler, James Szinger, James Balachandran, Harikrishnan Buzby, Adam Quinn, David Parks, Robert J. Tsao, Chun-Yen Carville, Angela Mansfield, Keith G. Pavlakis, George N. Felber, Barbara K. Haynes, Barton F. Korber, Bette T. Letvin, Norman L. Nat Med Article An effective HIV vaccine must elicit immune responses that recognize genetically diverse viruses1, 2. It must generate CD8+ T lymphocytes that control HIV replication and CD4+ T lymphocytes that provide help for the generation and maintenance of both cellular and humoral immune responses against the virus3–5. Creating immunogens that can elicit cellular immune responses against the genetically varied circulating isolates of HIV presents an important challenge for creating an AIDS vaccine6, 7. Polyvalent mosaic immunogens derived by in silico recombination of natural strains of HIV are designed to induce cellular immune responses that recognize genetically diverse circulating virus isolates8. In the present study we immunized rhesus monkeys by plasmid DNA prime/ recombinant vaccinia virus boost using vaccine constructs expressing either consensus or polyvalent mosaic proteins. The mosaic immunogens elicited CD8+ T lymphocyte responses to more epitopes of each viral protein than the consensus immunogens, and to more variant sequences of CD8+ T lymphocyte epitopes. This increased breadth and depth of epitope recognition may contribute both to protection against infection by genetically diverse viruses and to the control of variant viruses that emerge as they mutate away from recognition by cytotoxic T lymphocytes. 2010-02-21 2010-03 /pmc/articles/PMC2834806/ /pubmed/20173754 http://dx.doi.org/10.1038/nm.2108 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Santra, Sampa
Liao, Hua-Xin
Zhang, Ruijin
Muldoon, Mark
Watson, Sydeaka
Fischer, Will
Theiler, James
Szinger, James
Balachandran, Harikrishnan
Buzby, Adam
Quinn, David
Parks, Robert J.
Tsao, Chun-Yen
Carville, Angela
Mansfield, Keith G.
Pavlakis, George N.
Felber, Barbara K.
Haynes, Barton F.
Korber, Bette T.
Letvin, Norman L.
Mosaic Vaccines Elicit CD8+ T lymphocyte Responses in Monkeys that Confer Enhanced Immune Coverage of Diverse HIV Strains
title Mosaic Vaccines Elicit CD8+ T lymphocyte Responses in Monkeys that Confer Enhanced Immune Coverage of Diverse HIV Strains
title_full Mosaic Vaccines Elicit CD8+ T lymphocyte Responses in Monkeys that Confer Enhanced Immune Coverage of Diverse HIV Strains
title_fullStr Mosaic Vaccines Elicit CD8+ T lymphocyte Responses in Monkeys that Confer Enhanced Immune Coverage of Diverse HIV Strains
title_full_unstemmed Mosaic Vaccines Elicit CD8+ T lymphocyte Responses in Monkeys that Confer Enhanced Immune Coverage of Diverse HIV Strains
title_short Mosaic Vaccines Elicit CD8+ T lymphocyte Responses in Monkeys that Confer Enhanced Immune Coverage of Diverse HIV Strains
title_sort mosaic vaccines elicit cd8+ t lymphocyte responses in monkeys that confer enhanced immune coverage of diverse hiv strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2834806/
https://www.ncbi.nlm.nih.gov/pubmed/20173754
http://dx.doi.org/10.1038/nm.2108
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