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Robustness of the Bacterial Community in the Cabbage White Butterfly Larval Midgut

Microbial communities typically vary in composition and structure over space and time. Little is known about the inherent characteristics of communities that govern various drivers of these changes, such as random variation, changes in response to perturbation, or susceptibility to invasion. In this...

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Autores principales: Robinson, Courtney J., Schloss, Patrick, Ramos, Yolied, Raffa, Kenneth, Handelsman, Jo
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2836246/
https://www.ncbi.nlm.nih.gov/pubmed/19924467
http://dx.doi.org/10.1007/s00248-009-9595-8
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author Robinson, Courtney J.
Schloss, Patrick
Ramos, Yolied
Raffa, Kenneth
Handelsman, Jo
author_facet Robinson, Courtney J.
Schloss, Patrick
Ramos, Yolied
Raffa, Kenneth
Handelsman, Jo
author_sort Robinson, Courtney J.
collection PubMed
description Microbial communities typically vary in composition and structure over space and time. Little is known about the inherent characteristics of communities that govern various drivers of these changes, such as random variation, changes in response to perturbation, or susceptibility to invasion. In this study, we use 16S ribosomal RNA gene sequences to describe variation among bacterial communities in the midguts of cabbage white butterfly (Pieris rapae) larvae and examine the influence of community structure on susceptibility to invasion. We compared communities in larvae experiencing the same conditions at different times (temporal variation) or fed different diets (perturbation). The most highly represented phylum was Proteobacteria, which was present in all midgut communities. The observed species richness ranged from six to 15, and the most abundant members affiliated with the genera Methylobacteria, Asaia, Acinetobacter, Enterobacter, and Pantoea. Individual larvae subjected to the same conditions at the same time harbored communities that were highly similar in structure and membership, whereas the communities observed within larval populations changed with diet and over time. In addition, structural changes due to perturbation coincided with enhanced susceptibility to invasion by Enterobacter sp. NAB3R and Pantoea stewartii CWB600, suggesting that resistance to invasion is in part governed by community structure. These findings along with the observed conservation of membership at the phylum level, variation in structure and membership at lower taxonomic levels, and its relative simplicity make the cabbage white butterfly larval community an attractive model for studying community dynamics and robustness. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00248-009-9595-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-28362462010-03-18 Robustness of the Bacterial Community in the Cabbage White Butterfly Larval Midgut Robinson, Courtney J. Schloss, Patrick Ramos, Yolied Raffa, Kenneth Handelsman, Jo Microb Ecol Invertebrate Microbiology Microbial communities typically vary in composition and structure over space and time. Little is known about the inherent characteristics of communities that govern various drivers of these changes, such as random variation, changes in response to perturbation, or susceptibility to invasion. In this study, we use 16S ribosomal RNA gene sequences to describe variation among bacterial communities in the midguts of cabbage white butterfly (Pieris rapae) larvae and examine the influence of community structure on susceptibility to invasion. We compared communities in larvae experiencing the same conditions at different times (temporal variation) or fed different diets (perturbation). The most highly represented phylum was Proteobacteria, which was present in all midgut communities. The observed species richness ranged from six to 15, and the most abundant members affiliated with the genera Methylobacteria, Asaia, Acinetobacter, Enterobacter, and Pantoea. Individual larvae subjected to the same conditions at the same time harbored communities that were highly similar in structure and membership, whereas the communities observed within larval populations changed with diet and over time. In addition, structural changes due to perturbation coincided with enhanced susceptibility to invasion by Enterobacter sp. NAB3R and Pantoea stewartii CWB600, suggesting that resistance to invasion is in part governed by community structure. These findings along with the observed conservation of membership at the phylum level, variation in structure and membership at lower taxonomic levels, and its relative simplicity make the cabbage white butterfly larval community an attractive model for studying community dynamics and robustness. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00248-009-9595-8) contains supplementary material, which is available to authorized users. Springer-Verlag 2009-11-19 2010 /pmc/articles/PMC2836246/ /pubmed/19924467 http://dx.doi.org/10.1007/s00248-009-9595-8 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Invertebrate Microbiology
Robinson, Courtney J.
Schloss, Patrick
Ramos, Yolied
Raffa, Kenneth
Handelsman, Jo
Robustness of the Bacterial Community in the Cabbage White Butterfly Larval Midgut
title Robustness of the Bacterial Community in the Cabbage White Butterfly Larval Midgut
title_full Robustness of the Bacterial Community in the Cabbage White Butterfly Larval Midgut
title_fullStr Robustness of the Bacterial Community in the Cabbage White Butterfly Larval Midgut
title_full_unstemmed Robustness of the Bacterial Community in the Cabbage White Butterfly Larval Midgut
title_short Robustness of the Bacterial Community in the Cabbage White Butterfly Larval Midgut
title_sort robustness of the bacterial community in the cabbage white butterfly larval midgut
topic Invertebrate Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2836246/
https://www.ncbi.nlm.nih.gov/pubmed/19924467
http://dx.doi.org/10.1007/s00248-009-9595-8
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