Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF

We present a high throughput shotgun mass spectrometry workflow using a bidimensional peptide fractionation procedure consisting of isoelectric focusing and RP-HPLC prior to mass spectrometric analysis, with the aim of optimizing peptide separation and protein identification. As part of the workflow...

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Detalles Bibliográficos
Autores principales: Maccarrone, Giuseppina, Turck, Christoph W., Martins-de-Souza, Daniel
Formato: Texto
Lenguaje:English
Publicado: Springer US 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2836250/
https://www.ncbi.nlm.nih.gov/pubmed/20091422
http://dx.doi.org/10.1007/s10930-009-9227-7
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author Maccarrone, Giuseppina
Turck, Christoph W.
Martins-de-Souza, Daniel
author_facet Maccarrone, Giuseppina
Turck, Christoph W.
Martins-de-Souza, Daniel
author_sort Maccarrone, Giuseppina
collection PubMed
description We present a high throughput shotgun mass spectrometry workflow using a bidimensional peptide fractionation procedure consisting of isoelectric focusing and RP-HPLC prior to mass spectrometric analysis, with the aim of optimizing peptide separation and protein identification. As part of the workflow we used the ‘Isotope-Coded Protein Labeling’ (ICPL) method for accurate relative quantitation of protein expression. Such workflow was successfully applied to a comparative proteome analysis of schizophrenia versus healthy control brain tissues and can be an alternative to proteome researches.
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spelling pubmed-28362502010-03-18 Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF Maccarrone, Giuseppina Turck, Christoph W. Martins-de-Souza, Daniel Protein J Article We present a high throughput shotgun mass spectrometry workflow using a bidimensional peptide fractionation procedure consisting of isoelectric focusing and RP-HPLC prior to mass spectrometric analysis, with the aim of optimizing peptide separation and protein identification. As part of the workflow we used the ‘Isotope-Coded Protein Labeling’ (ICPL) method for accurate relative quantitation of protein expression. Such workflow was successfully applied to a comparative proteome analysis of schizophrenia versus healthy control brain tissues and can be an alternative to proteome researches. Springer US 2010-01-22 2010 /pmc/articles/PMC2836250/ /pubmed/20091422 http://dx.doi.org/10.1007/s10930-009-9227-7 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Maccarrone, Giuseppina
Turck, Christoph W.
Martins-de-Souza, Daniel
Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF
title Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF
title_full Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF
title_fullStr Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF
title_full_unstemmed Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF
title_short Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF
title_sort shotgun mass spectrometry workflow combining ief and lc-maldi-tof/tof
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2836250/
https://www.ncbi.nlm.nih.gov/pubmed/20091422
http://dx.doi.org/10.1007/s10930-009-9227-7
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