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Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF
We present a high throughput shotgun mass spectrometry workflow using a bidimensional peptide fractionation procedure consisting of isoelectric focusing and RP-HPLC prior to mass spectrometric analysis, with the aim of optimizing peptide separation and protein identification. As part of the workflow...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Springer US
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2836250/ https://www.ncbi.nlm.nih.gov/pubmed/20091422 http://dx.doi.org/10.1007/s10930-009-9227-7 |