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Effects of Liver × receptor agonist treatment on signal transduction pathways in acute lung inflammation
BACKGROUND: Liver × receptor α (LXRα) and β (LXRβ) are members of the nuclear receptor super family of ligand-activated transcription factors, a super family which includes the perhaps better known glucocorticoid receptor, estrogen receptor, thyroid receptor, and peroxisome proliferator-activated re...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2836283/ https://www.ncbi.nlm.nih.gov/pubmed/20175894 http://dx.doi.org/10.1186/1465-9921-11-19 |
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author | Crisafulli, Concetta Mazzon, Emanuela Paterniti, Irene Galuppo, Maria Bramanti, Placido Cuzzocrea, Salvatore |
author_facet | Crisafulli, Concetta Mazzon, Emanuela Paterniti, Irene Galuppo, Maria Bramanti, Placido Cuzzocrea, Salvatore |
author_sort | Crisafulli, Concetta |
collection | PubMed |
description | BACKGROUND: Liver × receptor α (LXRα) and β (LXRβ) are members of the nuclear receptor super family of ligand-activated transcription factors, a super family which includes the perhaps better known glucocorticoid receptor, estrogen receptor, thyroid receptor, and peroxisome proliferator-activated receptors. There is limited evidence that LXL activation may reduces acute lung inflammation. The aim of this study was to investigate the effects of T0901317, a potent LXR receptor ligand, in a mouse model of carrageenan-induced pleurisy. METHODS: Injection of carrageenan into the pleural cavity of mice elicited an acute inflammatory response characterized by: accumulation of fluid containing a large number of neutrophils (PMNs) in the pleural cavity, infiltration of PMNs in lung tissues and subsequent lipid peroxidation, and increased production of nitrite/nitrate (NOx), tumor necrosis factor-α, (TNF-α) and interleukin-1β (IL-1β). Furthermore, carrageenan induced the expression of iNOS, nitrotyrosine and PARP, as well as induced apoptosis (TUNEL staining and Bax and Bcl-2 expression) in the lung tissues. RESULTS: Administration of T0901317, 30 min after the challenge with carrageenan, caused a significant reduction in a dose dependent manner of all the parameters of inflammation measured. CONCLUSIONS: Thus, based on these findings we propose that LXR ligand such as T0901317, may be useful in the treatment of various inflammatory diseases. |
format | Text |
id | pubmed-2836283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-28362832010-03-11 Effects of Liver × receptor agonist treatment on signal transduction pathways in acute lung inflammation Crisafulli, Concetta Mazzon, Emanuela Paterniti, Irene Galuppo, Maria Bramanti, Placido Cuzzocrea, Salvatore Respir Res Research BACKGROUND: Liver × receptor α (LXRα) and β (LXRβ) are members of the nuclear receptor super family of ligand-activated transcription factors, a super family which includes the perhaps better known glucocorticoid receptor, estrogen receptor, thyroid receptor, and peroxisome proliferator-activated receptors. There is limited evidence that LXL activation may reduces acute lung inflammation. The aim of this study was to investigate the effects of T0901317, a potent LXR receptor ligand, in a mouse model of carrageenan-induced pleurisy. METHODS: Injection of carrageenan into the pleural cavity of mice elicited an acute inflammatory response characterized by: accumulation of fluid containing a large number of neutrophils (PMNs) in the pleural cavity, infiltration of PMNs in lung tissues and subsequent lipid peroxidation, and increased production of nitrite/nitrate (NOx), tumor necrosis factor-α, (TNF-α) and interleukin-1β (IL-1β). Furthermore, carrageenan induced the expression of iNOS, nitrotyrosine and PARP, as well as induced apoptosis (TUNEL staining and Bax and Bcl-2 expression) in the lung tissues. RESULTS: Administration of T0901317, 30 min after the challenge with carrageenan, caused a significant reduction in a dose dependent manner of all the parameters of inflammation measured. CONCLUSIONS: Thus, based on these findings we propose that LXR ligand such as T0901317, may be useful in the treatment of various inflammatory diseases. BioMed Central 2010 2010-02-22 /pmc/articles/PMC2836283/ /pubmed/20175894 http://dx.doi.org/10.1186/1465-9921-11-19 Text en Copyright ©2010 Crisafulli et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Crisafulli, Concetta Mazzon, Emanuela Paterniti, Irene Galuppo, Maria Bramanti, Placido Cuzzocrea, Salvatore Effects of Liver × receptor agonist treatment on signal transduction pathways in acute lung inflammation |
title | Effects of Liver × receptor agonist treatment on signal transduction pathways in acute lung inflammation |
title_full | Effects of Liver × receptor agonist treatment on signal transduction pathways in acute lung inflammation |
title_fullStr | Effects of Liver × receptor agonist treatment on signal transduction pathways in acute lung inflammation |
title_full_unstemmed | Effects of Liver × receptor agonist treatment on signal transduction pathways in acute lung inflammation |
title_short | Effects of Liver × receptor agonist treatment on signal transduction pathways in acute lung inflammation |
title_sort | effects of liver × receptor agonist treatment on signal transduction pathways in acute lung inflammation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2836283/ https://www.ncbi.nlm.nih.gov/pubmed/20175894 http://dx.doi.org/10.1186/1465-9921-11-19 |
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