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Streamlined, automated protocols for the production of milligram quantities of untagged recombinant human cyclophilin-A (hCypA) and untagged human proliferating cell nuclear antigen (hPCNA) using ÄKTAxpress™

We developed streamlined, automated purification protocols for the production of milligram quantities of untagged recombinant human cyclophilin-A (hCypA) and untagged human proliferating cell nuclear antigen (hPCNA) from Escherichia coli, using the ÄKTAxpress™ chromatography system. The automated 2-...

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Detalles Bibliográficos
Autores principales:	Ludwig, Cornelia, Wear, Martin A., Walkinshaw, Malcolm D.
Formato:	Texto
Lenguaje:	English
Publicado:	Academic Press 2010
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2837147/ https://www.ncbi.nlm.nih.gov/pubmed/19995609 http://dx.doi.org/10.1016/j.pep.2009.12.001

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