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HP1 Recruitment in the Absence of Argonaute Proteins in Drosophila

Highly repetitive and transposable element rich regions of the genome must be stabilized by the presence of heterochromatin. A direct role for RNA interference in the establishment of heterochromatin has been demonstrated in fission yeast. In metazoans, which possess multiple RNA–silencing pathways...

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Autores principales: Moshkovich, Nellie, Lei, Elissa P.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2837403/
https://www.ncbi.nlm.nih.gov/pubmed/20300658
http://dx.doi.org/10.1371/journal.pgen.1000880
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author Moshkovich, Nellie
Lei, Elissa P.
author_facet Moshkovich, Nellie
Lei, Elissa P.
author_sort Moshkovich, Nellie
collection PubMed
description Highly repetitive and transposable element rich regions of the genome must be stabilized by the presence of heterochromatin. A direct role for RNA interference in the establishment of heterochromatin has been demonstrated in fission yeast. In metazoans, which possess multiple RNA–silencing pathways that are both functionally distinct and spatially restricted, whether RNA silencing contributes directly to heterochromatin formation is not clear. Previous studies in Drosophila melanogaster have suggested the involvement of both the AGO2-dependent endogenous small interfering RNA (endo-siRNA) as well as Piwi-interacting RNA (piRNA) silencing pathways. In order to determine if these Argonaute genes are required for heterochromatin formation, we utilized transcriptional reporters and chromatin immunoprecipitation of the critical factor Heterochromatin Protein 1 (HP1) to monitor the heterochromatic state of piRNA clusters, which generate both endo-siRNAs and the bulk of piRNAs. Surprisingly, we find that mutation of AGO2 or piwi increases silencing at piRNA clusters corresponding to an increase of HP1 association. Furthermore, loss of piRNA production from a single piRNA cluster results in genome-wide redistribution of HP1 and reduction of silencing at a distant heterochromatic site, suggesting indirect effects on HP1 recruitment. Taken together, these results indicate that heterochromatin forms independently of endo-siRNA and piRNA pathways.
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spelling pubmed-28374032010-03-17 HP1 Recruitment in the Absence of Argonaute Proteins in Drosophila Moshkovich, Nellie Lei, Elissa P. PLoS Genet Research Article Highly repetitive and transposable element rich regions of the genome must be stabilized by the presence of heterochromatin. A direct role for RNA interference in the establishment of heterochromatin has been demonstrated in fission yeast. In metazoans, which possess multiple RNA–silencing pathways that are both functionally distinct and spatially restricted, whether RNA silencing contributes directly to heterochromatin formation is not clear. Previous studies in Drosophila melanogaster have suggested the involvement of both the AGO2-dependent endogenous small interfering RNA (endo-siRNA) as well as Piwi-interacting RNA (piRNA) silencing pathways. In order to determine if these Argonaute genes are required for heterochromatin formation, we utilized transcriptional reporters and chromatin immunoprecipitation of the critical factor Heterochromatin Protein 1 (HP1) to monitor the heterochromatic state of piRNA clusters, which generate both endo-siRNAs and the bulk of piRNAs. Surprisingly, we find that mutation of AGO2 or piwi increases silencing at piRNA clusters corresponding to an increase of HP1 association. Furthermore, loss of piRNA production from a single piRNA cluster results in genome-wide redistribution of HP1 and reduction of silencing at a distant heterochromatic site, suggesting indirect effects on HP1 recruitment. Taken together, these results indicate that heterochromatin forms independently of endo-siRNA and piRNA pathways. Public Library of Science 2010-03-12 /pmc/articles/PMC2837403/ /pubmed/20300658 http://dx.doi.org/10.1371/journal.pgen.1000880 Text en This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Moshkovich, Nellie
Lei, Elissa P.
HP1 Recruitment in the Absence of Argonaute Proteins in Drosophila
title HP1 Recruitment in the Absence of Argonaute Proteins in Drosophila
title_full HP1 Recruitment in the Absence of Argonaute Proteins in Drosophila
title_fullStr HP1 Recruitment in the Absence of Argonaute Proteins in Drosophila
title_full_unstemmed HP1 Recruitment in the Absence of Argonaute Proteins in Drosophila
title_short HP1 Recruitment in the Absence of Argonaute Proteins in Drosophila
title_sort hp1 recruitment in the absence of argonaute proteins in drosophila
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2837403/
https://www.ncbi.nlm.nih.gov/pubmed/20300658
http://dx.doi.org/10.1371/journal.pgen.1000880
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