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Dynamic Imaging of Experimental Leishmania donovani-Induced Hepatic Granulomas Detects Kupffer Cell-Restricted Antigen Presentation to Antigen-Specific CD8(+) T Cells
Kupffer cells (KCs) represent the major phagocytic population within the liver and provide an intracellular niche for the survival of a number of important human pathogens. Although KCs have been extensively studied in vitro, little is known of their in vivo response to infection and their capacity...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2837408/ https://www.ncbi.nlm.nih.gov/pubmed/20300603 http://dx.doi.org/10.1371/journal.ppat.1000805 |
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author | Beattie, Lynette Peltan, Adam Maroof, Asher Kirby, Alun Brown, Najmeeyah Coles, Mark Smith, Deborah F. Kaye, Paul M. |
author_facet | Beattie, Lynette Peltan, Adam Maroof, Asher Kirby, Alun Brown, Najmeeyah Coles, Mark Smith, Deborah F. Kaye, Paul M. |
author_sort | Beattie, Lynette |
collection | PubMed |
description | Kupffer cells (KCs) represent the major phagocytic population within the liver and provide an intracellular niche for the survival of a number of important human pathogens. Although KCs have been extensively studied in vitro, little is known of their in vivo response to infection and their capacity to directly interact with antigen-specific CD8(+) T cells. Here, using a combination of approaches including whole mount and thin section confocal microscopy, adoptive cell transfer and intra-vital 2-photon microscopy, we demonstrate that KCs represent the only detectable population of mononuclear phagocytes within granulomas induced by Leishmania donovani infection that are capable of presenting parasite-derived peptide to effector CD8(+) T cells. This restriction of antigen presentation to KCs within the Leishmania granuloma has important implications for the identification of new candidate vaccine antigens and for the design of novel immuno-therapeutic interventions. |
format | Text |
id | pubmed-2837408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-28374082010-03-17 Dynamic Imaging of Experimental Leishmania donovani-Induced Hepatic Granulomas Detects Kupffer Cell-Restricted Antigen Presentation to Antigen-Specific CD8(+) T Cells Beattie, Lynette Peltan, Adam Maroof, Asher Kirby, Alun Brown, Najmeeyah Coles, Mark Smith, Deborah F. Kaye, Paul M. PLoS Pathog Research Article Kupffer cells (KCs) represent the major phagocytic population within the liver and provide an intracellular niche for the survival of a number of important human pathogens. Although KCs have been extensively studied in vitro, little is known of their in vivo response to infection and their capacity to directly interact with antigen-specific CD8(+) T cells. Here, using a combination of approaches including whole mount and thin section confocal microscopy, adoptive cell transfer and intra-vital 2-photon microscopy, we demonstrate that KCs represent the only detectable population of mononuclear phagocytes within granulomas induced by Leishmania donovani infection that are capable of presenting parasite-derived peptide to effector CD8(+) T cells. This restriction of antigen presentation to KCs within the Leishmania granuloma has important implications for the identification of new candidate vaccine antigens and for the design of novel immuno-therapeutic interventions. Public Library of Science 2010-03-12 /pmc/articles/PMC2837408/ /pubmed/20300603 http://dx.doi.org/10.1371/journal.ppat.1000805 Text en Beattie et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Beattie, Lynette Peltan, Adam Maroof, Asher Kirby, Alun Brown, Najmeeyah Coles, Mark Smith, Deborah F. Kaye, Paul M. Dynamic Imaging of Experimental Leishmania donovani-Induced Hepatic Granulomas Detects Kupffer Cell-Restricted Antigen Presentation to Antigen-Specific CD8(+) T Cells |
title | Dynamic Imaging of Experimental Leishmania donovani-Induced Hepatic Granulomas Detects Kupffer Cell-Restricted Antigen Presentation to Antigen-Specific CD8(+) T Cells |
title_full | Dynamic Imaging of Experimental Leishmania donovani-Induced Hepatic Granulomas Detects Kupffer Cell-Restricted Antigen Presentation to Antigen-Specific CD8(+) T Cells |
title_fullStr | Dynamic Imaging of Experimental Leishmania donovani-Induced Hepatic Granulomas Detects Kupffer Cell-Restricted Antigen Presentation to Antigen-Specific CD8(+) T Cells |
title_full_unstemmed | Dynamic Imaging of Experimental Leishmania donovani-Induced Hepatic Granulomas Detects Kupffer Cell-Restricted Antigen Presentation to Antigen-Specific CD8(+) T Cells |
title_short | Dynamic Imaging of Experimental Leishmania donovani-Induced Hepatic Granulomas Detects Kupffer Cell-Restricted Antigen Presentation to Antigen-Specific CD8(+) T Cells |
title_sort | dynamic imaging of experimental leishmania donovani-induced hepatic granulomas detects kupffer cell-restricted antigen presentation to antigen-specific cd8(+) t cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2837408/ https://www.ncbi.nlm.nih.gov/pubmed/20300603 http://dx.doi.org/10.1371/journal.ppat.1000805 |
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