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Molecular Evolution of Human H1N1 and H3N2 Influenza A Virus in Thailand, 2006–2009

BACKGROUND: Annual seasonal influenza outbreaks are associated with high morbidity and mortality. OBJECTIVE: To index and document evolutionary changes among influenza A H1N1 and H3N2 viruses isolated from Thailand during 2006–2009, using complete genome sequences. METHODS: Nasopharyngeal aspirates...

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Autores principales: Suwannakarn, Kamol, Chieochansin, Thaweesak, Thongmee, Chitima, Makkoch, Jarika, Praianantathavorn, Kesmanee, Theamboonlers, Apiradee, Sreevatsan, Srinand, Poovorawan, Yong
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838802/
https://www.ncbi.nlm.nih.gov/pubmed/20300536
http://dx.doi.org/10.1371/journal.pone.0009717
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author Suwannakarn, Kamol
Chieochansin, Thaweesak
Thongmee, Chitima
Makkoch, Jarika
Praianantathavorn, Kesmanee
Theamboonlers, Apiradee
Sreevatsan, Srinand
Poovorawan, Yong
author_facet Suwannakarn, Kamol
Chieochansin, Thaweesak
Thongmee, Chitima
Makkoch, Jarika
Praianantathavorn, Kesmanee
Theamboonlers, Apiradee
Sreevatsan, Srinand
Poovorawan, Yong
author_sort Suwannakarn, Kamol
collection PubMed
description BACKGROUND: Annual seasonal influenza outbreaks are associated with high morbidity and mortality. OBJECTIVE: To index and document evolutionary changes among influenza A H1N1 and H3N2 viruses isolated from Thailand during 2006–2009, using complete genome sequences. METHODS: Nasopharyngeal aspirates were collected from patients diagnosed with respiratory illness in Thailand during 2006–2009. All samples were screened for Influenza A virus. A total of 13 H1N1 and 21 H3N2 were confirmed and whole genome sequenced for the evolutionary analysis using standard phylogenetic approaches. RESULTS: Phylogenetic analysis of HA revealed a clear diversification of seasonal from vaccine strain lineages. H3N2 seasonal clusters were closely related to the WHO recommended vaccine strains in each season. Most H1N1 isolates could be differentiated into 3 lineages. The A/Brisbane/59/2007 lineage, a vaccine strain for H1N1 since 2008, is closely related with the H1N1 subtypes circulating in 2009. HA sequences were conserved at the receptor-binding site. Amino acid variations in the antigenic site resulted in a possible N-linked glycosylation motif. Recent H3N2 isolates had higher genetic variations compared to H1N1 isolates. Most substitutions in the NP protein were clustered in the T-cell recognition domains. CONCLUSION: In this study we performed evolutionary genetic analysis of influenza A viruses in Thailand between 2006–2009. Although the current vaccine strain is efficient for controlling the circulating outbreak subtypes, surveillance is necessary to provide unambiguous information on emergent viruses. In summary, the findings of this study contribute the understanding of evolution in influenza A viruses in humans and is useful for routine surveillance and vaccine strain selection.
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spelling pubmed-28388022010-03-19 Molecular Evolution of Human H1N1 and H3N2 Influenza A Virus in Thailand, 2006–2009 Suwannakarn, Kamol Chieochansin, Thaweesak Thongmee, Chitima Makkoch, Jarika Praianantathavorn, Kesmanee Theamboonlers, Apiradee Sreevatsan, Srinand Poovorawan, Yong PLoS One Research Article BACKGROUND: Annual seasonal influenza outbreaks are associated with high morbidity and mortality. OBJECTIVE: To index and document evolutionary changes among influenza A H1N1 and H3N2 viruses isolated from Thailand during 2006–2009, using complete genome sequences. METHODS: Nasopharyngeal aspirates were collected from patients diagnosed with respiratory illness in Thailand during 2006–2009. All samples were screened for Influenza A virus. A total of 13 H1N1 and 21 H3N2 were confirmed and whole genome sequenced for the evolutionary analysis using standard phylogenetic approaches. RESULTS: Phylogenetic analysis of HA revealed a clear diversification of seasonal from vaccine strain lineages. H3N2 seasonal clusters were closely related to the WHO recommended vaccine strains in each season. Most H1N1 isolates could be differentiated into 3 lineages. The A/Brisbane/59/2007 lineage, a vaccine strain for H1N1 since 2008, is closely related with the H1N1 subtypes circulating in 2009. HA sequences were conserved at the receptor-binding site. Amino acid variations in the antigenic site resulted in a possible N-linked glycosylation motif. Recent H3N2 isolates had higher genetic variations compared to H1N1 isolates. Most substitutions in the NP protein were clustered in the T-cell recognition domains. CONCLUSION: In this study we performed evolutionary genetic analysis of influenza A viruses in Thailand between 2006–2009. Although the current vaccine strain is efficient for controlling the circulating outbreak subtypes, surveillance is necessary to provide unambiguous information on emergent viruses. In summary, the findings of this study contribute the understanding of evolution in influenza A viruses in humans and is useful for routine surveillance and vaccine strain selection. Public Library of Science 2010-03-16 /pmc/articles/PMC2838802/ /pubmed/20300536 http://dx.doi.org/10.1371/journal.pone.0009717 Text en Suwannakarn et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Suwannakarn, Kamol
Chieochansin, Thaweesak
Thongmee, Chitima
Makkoch, Jarika
Praianantathavorn, Kesmanee
Theamboonlers, Apiradee
Sreevatsan, Srinand
Poovorawan, Yong
Molecular Evolution of Human H1N1 and H3N2 Influenza A Virus in Thailand, 2006–2009
title Molecular Evolution of Human H1N1 and H3N2 Influenza A Virus in Thailand, 2006–2009
title_full Molecular Evolution of Human H1N1 and H3N2 Influenza A Virus in Thailand, 2006–2009
title_fullStr Molecular Evolution of Human H1N1 and H3N2 Influenza A Virus in Thailand, 2006–2009
title_full_unstemmed Molecular Evolution of Human H1N1 and H3N2 Influenza A Virus in Thailand, 2006–2009
title_short Molecular Evolution of Human H1N1 and H3N2 Influenza A Virus in Thailand, 2006–2009
title_sort molecular evolution of human h1n1 and h3n2 influenza a virus in thailand, 2006–2009
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838802/
https://www.ncbi.nlm.nih.gov/pubmed/20300536
http://dx.doi.org/10.1371/journal.pone.0009717
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