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Expression of ZIC family genes in meningiomas and other brain tumors

BACKGROUND: Zic zinc finger proteins are present in the developing rodent meninges and are required for cell proliferation and differentiation of meningeal progenitors. Although human ZIC genes are known to be molecular markers for medulloblastomas, their expression in meningioma has not been addres...

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Autores principales: Aruga, Jun, Nozaki, Yayoi, Hatayama, Minoru, Odaka, Yuri S, Yokota, Naoki
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838823/
https://www.ncbi.nlm.nih.gov/pubmed/20199689
http://dx.doi.org/10.1186/1471-2407-10-79
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author Aruga, Jun
Nozaki, Yayoi
Hatayama, Minoru
Odaka, Yuri S
Yokota, Naoki
author_facet Aruga, Jun
Nozaki, Yayoi
Hatayama, Minoru
Odaka, Yuri S
Yokota, Naoki
author_sort Aruga, Jun
collection PubMed
description BACKGROUND: Zic zinc finger proteins are present in the developing rodent meninges and are required for cell proliferation and differentiation of meningeal progenitors. Although human ZIC genes are known to be molecular markers for medulloblastomas, their expression in meningioma has not been addressed to date. METHODS: We examined the mRNA and protein expression of human ZIC1, ZIC2, ZIC3, ZIC4 and ZIC5 genes in meningiomas in comparison to other brain tumors, using RT-PCR, analysis of published microarray data, and immunostaining. RESULTS: ZIC1, ZIC2 and ZIC5 transcript levels in meningiomas were higher than those in whole brain or normal dura mater, whereas all five ZIC genes were abundantly expressed in medulloblastomas. The expression level of ZIC1 in public microarray data was greater in meningiomas classified as World Health Organization Grade II (atypical) than those classified as Grade I (benign). Immunoscreening using anti-ZIC antibodies revealed that 23 out of 23 meningioma cases were ZIC1/2/3/5-immunopositive. By comparison, nuclear staining by the anti-ZIC4 antibody was not observed in any meningioma case, but was strongly detected in all four medulloblastomas. ZIC-positive meningiomas included meningothelial, fibrous, transitional, and psammomatous histological subtypes. In normal meninges, ZIC-like immunoreactivities were detected in vimentin-expressing arachnoid cells both in human and mouse. CONCLUSIONS: ZIC1, ZIC2, and ZIC5 are novel molecular markers for meningiomas whereas ZIC4 expression is highly selective for medulloblastomas. The pattern of ZIC expression in both of these tumor types may reflect the properties of the tissues from which the tumors are derived.
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spelling pubmed-28388232010-03-16 Expression of ZIC family genes in meningiomas and other brain tumors Aruga, Jun Nozaki, Yayoi Hatayama, Minoru Odaka, Yuri S Yokota, Naoki BMC Cancer Research Article BACKGROUND: Zic zinc finger proteins are present in the developing rodent meninges and are required for cell proliferation and differentiation of meningeal progenitors. Although human ZIC genes are known to be molecular markers for medulloblastomas, their expression in meningioma has not been addressed to date. METHODS: We examined the mRNA and protein expression of human ZIC1, ZIC2, ZIC3, ZIC4 and ZIC5 genes in meningiomas in comparison to other brain tumors, using RT-PCR, analysis of published microarray data, and immunostaining. RESULTS: ZIC1, ZIC2 and ZIC5 transcript levels in meningiomas were higher than those in whole brain or normal dura mater, whereas all five ZIC genes were abundantly expressed in medulloblastomas. The expression level of ZIC1 in public microarray data was greater in meningiomas classified as World Health Organization Grade II (atypical) than those classified as Grade I (benign). Immunoscreening using anti-ZIC antibodies revealed that 23 out of 23 meningioma cases were ZIC1/2/3/5-immunopositive. By comparison, nuclear staining by the anti-ZIC4 antibody was not observed in any meningioma case, but was strongly detected in all four medulloblastomas. ZIC-positive meningiomas included meningothelial, fibrous, transitional, and psammomatous histological subtypes. In normal meninges, ZIC-like immunoreactivities were detected in vimentin-expressing arachnoid cells both in human and mouse. CONCLUSIONS: ZIC1, ZIC2, and ZIC5 are novel molecular markers for meningiomas whereas ZIC4 expression is highly selective for medulloblastomas. The pattern of ZIC expression in both of these tumor types may reflect the properties of the tissues from which the tumors are derived. BioMed Central 2010-03-03 /pmc/articles/PMC2838823/ /pubmed/20199689 http://dx.doi.org/10.1186/1471-2407-10-79 Text en Copyright ©2010 Aruga et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Aruga, Jun
Nozaki, Yayoi
Hatayama, Minoru
Odaka, Yuri S
Yokota, Naoki
Expression of ZIC family genes in meningiomas and other brain tumors
title Expression of ZIC family genes in meningiomas and other brain tumors
title_full Expression of ZIC family genes in meningiomas and other brain tumors
title_fullStr Expression of ZIC family genes in meningiomas and other brain tumors
title_full_unstemmed Expression of ZIC family genes in meningiomas and other brain tumors
title_short Expression of ZIC family genes in meningiomas and other brain tumors
title_sort expression of zic family genes in meningiomas and other brain tumors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2838823/
https://www.ncbi.nlm.nih.gov/pubmed/20199689
http://dx.doi.org/10.1186/1471-2407-10-79
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