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Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease

One-day-old SPF chicks were inoculated with the Cux-l strain of chicken infectious anemia virus (CIAV), and the clinical development of disease and its macroscopic and microscopic alterations in the thymus and bone marrow, were observed. Tissue sections of thymus and bone marrow were stained using t...

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Autores principales: Kuscu, Burak, Gürel, Aydin
Formato: Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839108/
https://www.ncbi.nlm.nih.gov/pubmed/18296884
http://dx.doi.org/10.4142/jvs.2008.9.1.15
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author Kuscu, Burak
Gürel, Aydin
author_facet Kuscu, Burak
Gürel, Aydin
author_sort Kuscu, Burak
collection PubMed
description One-day-old SPF chicks were inoculated with the Cux-l strain of chicken infectious anemia virus (CIAV), and the clinical development of disease and its macroscopic and microscopic alterations in the thymus and bone marrow, were observed. Tissue sections of thymus and bone marrow were stained using the streptavidin-biotin peroxidase method and examined under light microscope for evaluation of antigenic intensities in tissues. Those findings were then compared with blood parameters and ELISA results obtained through collected sera during sacrifice procedures. We sought to determine: the localization of viral antigens in thymus and bone marrow tissues after inoculation, the correlation between antigen intensities and hematologic, serologic and histopathologic findings, definitive diagnostic criteria using histopathologic and immunoperoxidase methods, and the reliability of these methods in the diagnosis of CIAV infection. For this purpose, 83, one-day-old SPF chicks were used. The birds were divided into experimental (n = 52) and control (n = 26) groups. A virus dose of TCID50 of 100,000/ml was administered intramuscularly to every bird in the experimental group. Based on the results of this study, we have suggested that clinical examination, along with macroscopic and microscopic evaluation of the thymus and bone marrow, maybe undertaken starting from day 7 post-inoculation (PI). ELISA, might be of value, as it might give consistent results starting from day 14 PI. However, the most reliable results were obtained through examination of thymus and bone marrow sections from infected birds stained by immunoperoxidase technique, as early as day 4 PI.
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spelling pubmed-28391082010-03-16 Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease Kuscu, Burak Gürel, Aydin J Vet Sci Original Article One-day-old SPF chicks were inoculated with the Cux-l strain of chicken infectious anemia virus (CIAV), and the clinical development of disease and its macroscopic and microscopic alterations in the thymus and bone marrow, were observed. Tissue sections of thymus and bone marrow were stained using the streptavidin-biotin peroxidase method and examined under light microscope for evaluation of antigenic intensities in tissues. Those findings were then compared with blood parameters and ELISA results obtained through collected sera during sacrifice procedures. We sought to determine: the localization of viral antigens in thymus and bone marrow tissues after inoculation, the correlation between antigen intensities and hematologic, serologic and histopathologic findings, definitive diagnostic criteria using histopathologic and immunoperoxidase methods, and the reliability of these methods in the diagnosis of CIAV infection. For this purpose, 83, one-day-old SPF chicks were used. The birds were divided into experimental (n = 52) and control (n = 26) groups. A virus dose of TCID50 of 100,000/ml was administered intramuscularly to every bird in the experimental group. Based on the results of this study, we have suggested that clinical examination, along with macroscopic and microscopic evaluation of the thymus and bone marrow, maybe undertaken starting from day 7 post-inoculation (PI). ELISA, might be of value, as it might give consistent results starting from day 14 PI. However, the most reliable results were obtained through examination of thymus and bone marrow sections from infected birds stained by immunoperoxidase technique, as early as day 4 PI. The Korean Society of Veterinary Science 2008-03 2008-03-31 /pmc/articles/PMC2839108/ /pubmed/18296884 http://dx.doi.org/10.4142/jvs.2008.9.1.15 Text en Copyright © 2008 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kuscu, Burak
Gürel, Aydin
Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease
title Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease
title_full Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease
title_fullStr Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease
title_full_unstemmed Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease
title_short Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease
title_sort lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839108/
https://www.ncbi.nlm.nih.gov/pubmed/18296884
http://dx.doi.org/10.4142/jvs.2008.9.1.15
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