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Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope

Nuclear envelope complexity is expanding with respect to identification of protein components. Here we test the validity of proteomics results that identified 67 novel predicted nuclear envelope transmembrane proteins (NETs) from liver by directly comparing 30 as tagged fusions using targeting assay...

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Autores principales: Malik, Poonam, Korfali, Nadia, Srsen, Vlastimil, Lazou, Vassiliki, Batrakou, Dzmitry G., Zuleger, Nikolaj, Kavanagh, Deirdre M., Wilkie, Gavin S., Goldberg, Martin W., Schirmer, Eric C.
Formato: Texto
Lenguaje:English
Publicado: SP Birkhäuser Verlag Basel 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839517/
https://www.ncbi.nlm.nih.gov/pubmed/20091084
http://dx.doi.org/10.1007/s00018-010-0257-2
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author Malik, Poonam
Korfali, Nadia
Srsen, Vlastimil
Lazou, Vassiliki
Batrakou, Dzmitry G.
Zuleger, Nikolaj
Kavanagh, Deirdre M.
Wilkie, Gavin S.
Goldberg, Martin W.
Schirmer, Eric C.
author_facet Malik, Poonam
Korfali, Nadia
Srsen, Vlastimil
Lazou, Vassiliki
Batrakou, Dzmitry G.
Zuleger, Nikolaj
Kavanagh, Deirdre M.
Wilkie, Gavin S.
Goldberg, Martin W.
Schirmer, Eric C.
author_sort Malik, Poonam
collection PubMed
description Nuclear envelope complexity is expanding with respect to identification of protein components. Here we test the validity of proteomics results that identified 67 novel predicted nuclear envelope transmembrane proteins (NETs) from liver by directly comparing 30 as tagged fusions using targeting assays. This confirmed 21 as NETs, but 4 only targeted in certain cell types, underscoring the complexity of interactions that tether NETs to the nuclear envelope. Four NETs accumulated at the nuclear rim in normal fibroblasts but not in fibroblasts lacking lamin A, suggesting involvement of lamin A in tethering them in the nucleus. However, intriguingly, for the NETs tested alternative mechanisms for nuclear envelope retention could be found in Jurkat cells that normally lack lamin A. This study expands by a factor of three the number of liver NETs analyzed, bringing the total confirmed to 31, and shows that several have multiple mechanisms for nuclear envelope retention.
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spelling pubmed-28395172010-03-26 Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope Malik, Poonam Korfali, Nadia Srsen, Vlastimil Lazou, Vassiliki Batrakou, Dzmitry G. Zuleger, Nikolaj Kavanagh, Deirdre M. Wilkie, Gavin S. Goldberg, Martin W. Schirmer, Eric C. Cell Mol Life Sci Research Article Nuclear envelope complexity is expanding with respect to identification of protein components. Here we test the validity of proteomics results that identified 67 novel predicted nuclear envelope transmembrane proteins (NETs) from liver by directly comparing 30 as tagged fusions using targeting assays. This confirmed 21 as NETs, but 4 only targeted in certain cell types, underscoring the complexity of interactions that tether NETs to the nuclear envelope. Four NETs accumulated at the nuclear rim in normal fibroblasts but not in fibroblasts lacking lamin A, suggesting involvement of lamin A in tethering them in the nucleus. However, intriguingly, for the NETs tested alternative mechanisms for nuclear envelope retention could be found in Jurkat cells that normally lack lamin A. This study expands by a factor of three the number of liver NETs analyzed, bringing the total confirmed to 31, and shows that several have multiple mechanisms for nuclear envelope retention. SP Birkhäuser Verlag Basel 2010-01-21 2010 /pmc/articles/PMC2839517/ /pubmed/20091084 http://dx.doi.org/10.1007/s00018-010-0257-2 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Research Article
Malik, Poonam
Korfali, Nadia
Srsen, Vlastimil
Lazou, Vassiliki
Batrakou, Dzmitry G.
Zuleger, Nikolaj
Kavanagh, Deirdre M.
Wilkie, Gavin S.
Goldberg, Martin W.
Schirmer, Eric C.
Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope
title Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope
title_full Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope
title_fullStr Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope
title_full_unstemmed Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope
title_short Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope
title_sort cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839517/
https://www.ncbi.nlm.nih.gov/pubmed/20091084
http://dx.doi.org/10.1007/s00018-010-0257-2
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