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Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells

Dendritic cells (DC) represent a heterogeneous cell family of major importance for innate immune responses against pathogens and antigen presentation during infection, cancer, allergy and autoimmunity. The aim of the present study was to characterize canine DC generated in vitro with respect to thei...

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Autores principales: Ricklin Gutzwiller, Meret Elisabeth, Moulin, Hervé Raphaël, Zurbriggen, Andreas, Roosje, Petra, Summerfield, Artur
Formato: Texto
Lenguaje:English
Publicado: EDP Sciences 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839791/
https://www.ncbi.nlm.nih.gov/pubmed/20167201
http://dx.doi.org/10.1051/vetres/2010012
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author Ricklin Gutzwiller, Meret Elisabeth
Moulin, Hervé Raphaël
Zurbriggen, Andreas
Roosje, Petra
Summerfield, Artur
author_facet Ricklin Gutzwiller, Meret Elisabeth
Moulin, Hervé Raphaël
Zurbriggen, Andreas
Roosje, Petra
Summerfield, Artur
author_sort Ricklin Gutzwiller, Meret Elisabeth
collection PubMed
description Dendritic cells (DC) represent a heterogeneous cell family of major importance for innate immune responses against pathogens and antigen presentation during infection, cancer, allergy and autoimmunity. The aim of the present study was to characterize canine DC generated in vitro with respect to their phenotype, responsiveness to toll-like receptor (TLR) ligands and T-cell stimulatory capacity. DC were derived from monocytes (MoDC) and from bone marrow hematopoietic cells cultured with either Flt3-ligand (FL-BMDC) or with GM-CSF (GM-BMDC). All three methods generated cells with typical DC morphology that expressed CD1c, CD11c and CD14, similar to macrophages. However, CD40 was only found on DC, CD206 on MΦ and BMDC, but not on monocytes and MoDC. CD1c was not found on monocytes but on all in vitro differentiated cells. FL-BMDC and GM-BMDC were partially positive for CD4 and CD8. CD45RA was expressed on a subset of FL-BMDC but not on MoDC and GM-BMDC. MoDC and FL-DC responded well to TLR ligands including poly-IC (TLR2), Pam3Cys (TLR3), LPS (TLR4) and imiquimod (TLR7) by up-regulating MHC II and CD86. The generated DC and MΦ showed a stimulatory capacity for lymphocytes, which increased upon maturation with LPS. Taken together, our results are the basis for further characterization of canine DC subsets with respect to their role in inflammation and immune responses.
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spelling pubmed-28397912011-07-01 Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells Ricklin Gutzwiller, Meret Elisabeth Moulin, Hervé Raphaël Zurbriggen, Andreas Roosje, Petra Summerfield, Artur Vet Res Original Article Dendritic cells (DC) represent a heterogeneous cell family of major importance for innate immune responses against pathogens and antigen presentation during infection, cancer, allergy and autoimmunity. The aim of the present study was to characterize canine DC generated in vitro with respect to their phenotype, responsiveness to toll-like receptor (TLR) ligands and T-cell stimulatory capacity. DC were derived from monocytes (MoDC) and from bone marrow hematopoietic cells cultured with either Flt3-ligand (FL-BMDC) or with GM-CSF (GM-BMDC). All three methods generated cells with typical DC morphology that expressed CD1c, CD11c and CD14, similar to macrophages. However, CD40 was only found on DC, CD206 on MΦ and BMDC, but not on monocytes and MoDC. CD1c was not found on monocytes but on all in vitro differentiated cells. FL-BMDC and GM-BMDC were partially positive for CD4 and CD8. CD45RA was expressed on a subset of FL-BMDC but not on MoDC and GM-BMDC. MoDC and FL-DC responded well to TLR ligands including poly-IC (TLR2), Pam3Cys (TLR3), LPS (TLR4) and imiquimod (TLR7) by up-regulating MHC II and CD86. The generated DC and MΦ showed a stimulatory capacity for lymphocytes, which increased upon maturation with LPS. Taken together, our results are the basis for further characterization of canine DC subsets with respect to their role in inflammation and immune responses. EDP Sciences 2010-02-22 2010 /pmc/articles/PMC2839791/ /pubmed/20167201 http://dx.doi.org/10.1051/vetres/2010012 Text en © INRA, EDP Sciences, 2010
spellingShingle Original Article
Ricklin Gutzwiller, Meret Elisabeth
Moulin, Hervé Raphaël
Zurbriggen, Andreas
Roosje, Petra
Summerfield, Artur
Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells
title Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells
title_full Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells
title_fullStr Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells
title_full_unstemmed Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells
title_short Comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells
title_sort comparative analysis of canine monocyte- and bone-marrow-derived dendritic cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2839791/
https://www.ncbi.nlm.nih.gov/pubmed/20167201
http://dx.doi.org/10.1051/vetres/2010012
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